From 4d6ad0c6c3432627155292ddefba029149fbcc58 Mon Sep 17 00:00:00 2001 From: ShixiangWang Date: Wed, 30 Jan 2019 22:39:23 +0800 Subject: [PATCH] release v0.1.5 --- NAMESPACE | 1 + NEWS.md | 6 +- R/draw_series.R | 98 +++++---------- R/get_series.R | 39 +++--- R/prepare_series.R | 9 +- R/read_series.R | 57 ++++----- R/signature_series.R | 126 ++++++++------------ README.Rmd | 6 +- README.md | 10 +- inst/extdata/toy_cn.RData | Bin 0 -> 11059 bytes inst/extdata/toy_cn_components.RData | Bin 0 -> 184202 bytes inst/extdata/toy_cn_features.RData | Bin 0 -> 5110 bytes inst/extdata/toy_cnlist.RData | Bin 0 -> 6037 bytes inst/extdata/toy_copynumber.RData | Bin 0 -> 9476 bytes inst/extdata/toy_copynumber_prepare.RData | Bin 0 -> 279974 bytes inst/extdata/toy_copynumber_signature.RData | Bin 0 -> 4700 bytes inst/extdata/toy_segTab.RData | Bin 0 -> 4054 bytes inst/test/develop.R | 25 +++- man/draw_cn_components.Rd | 12 +- man/draw_cn_distribution.Rd | 12 +- man/draw_cn_features.Rd | 12 +- man/draw_sig_activity.Rd | 13 +- man/draw_sig_corrplot.Rd | 11 +- man/draw_sig_profile.Rd | 13 +- man/draw_subtypes_comparison.Rd | 25 ++-- man/figures/logo.png | Bin 0 -> 43137 bytes man/get_LengthFraction.Rd | 8 +- man/get_cnlist.Rd | 2 - man/get_components.Rd | 7 +- man/get_features.Rd | 8 +- man/get_matrix.Rd | 14 +-- man/prepare_copynumber.Rd | 9 +- man/read_copynumber.Rd | 10 +- man/read_maf.Rd | 4 +- man/read_variation.Rd | 10 +- man/sig_assign_samples.Rd | 23 ++-- man/sig_estimate.Rd | 10 +- man/sig_extract.Rd | 13 +- man/sig_get_activity.Rd | 14 +-- man/sig_get_correlation.Rd | 15 +-- man/sig_prepare.Rd | 25 ++-- man/sig_summarize_subtypes.Rd | 23 ++-- 42 files changed, 263 insertions(+), 407 deletions(-) create mode 100644 inst/extdata/toy_cn.RData create mode 100644 inst/extdata/toy_cn_components.RData create mode 100644 inst/extdata/toy_cn_features.RData create mode 100644 inst/extdata/toy_cnlist.RData create mode 100644 inst/extdata/toy_copynumber.RData create mode 100644 inst/extdata/toy_copynumber_prepare.RData create mode 100644 inst/extdata/toy_copynumber_signature.RData create mode 100644 inst/extdata/toy_segTab.RData create mode 100644 man/figures/logo.png diff --git a/NAMESPACE b/NAMESPACE index 00b4bafb..bc105e6c 100644 --- a/NAMESPACE +++ b/NAMESPACE @@ -22,6 +22,7 @@ export(get_matrix) export(hello) export(prepare_copynumber) export(read_copynumber) +export(read_maf) export(read_variation) export(sig_assign_samples) export(sig_estimate) diff --git a/NEWS.md b/NEWS.md index 85f7e6ed..48e14b00 100644 --- a/NEWS.md +++ b/NEWS.md @@ -1,8 +1,12 @@ # sigminer v0.1.5 -* update intro for package +* update introduction for package `sigminer` * fix text problem provided by CRAN check team * add `sig_get_similarity` function +* add toy datasets & update examples +* fix typo in `sig_assign_samples` +* export `read_maf` +* update README # sigminer v0.1.4 diff --git a/R/draw_series.R b/R/draw_series.R index 81589fcd..b6d80d47 100644 --- a/R/draw_series.R +++ b/R/draw_series.R @@ -18,17 +18,13 @@ #' @import ggplot2 #' @export #' @examples -#' \donttest{ -#' load(system.file("extdata", "example_cn_list.RData", -#' package = "sigminer", mustWork = TRUE)) -#' segTabs = data.table::rbindlist(tcga_segTabs, idcol = "sample") -#' cn = read_copynumber(segTabs, -#' seg_cols = c("chromosome", "start", "end", "segVal"), -#' genome_build = "hg19") +#' # Load copy number object +#' load(system.file("extdata", "toy_copynumber.RData", +#' package = "sigminer", mustWork = TRUE)) +#' # Plot distribution #' draw_cn_distribution(cn) #' draw_cn_distribution(cn, mode = "cd") #' draw_cn_distribution(cn, mode = "cd", fill = TRUE) -#' } #' @family copy number plot draw_cn_distribution = function(data, rm_normal = TRUE, @@ -169,16 +165,10 @@ draw_cn_distribution = function(data, #' @import ggplot2 #' @family copy number plot #' @examples -#' \donttest{ -#' load(system.file("extdata", "example_cn_list.RData", -#' package = "sigminer", mustWork = TRUE)) -#' segTabs = data.table::rbindlist(tcga_segTabs, idcol = "sample") -#' cn = read_copynumber(segTabs, -#' seg_cols = c("chromosome", "start", "end", "segVal"), -#' genome_build = "hg19") -#' cn_prepare = sig_prepare(cn) +#' # Load copy number prepare object +#' load(system.file("extdata", "toy_copynumber_prepare.RData", +#' package = "sigminer", mustWork = TRUE)) #' draw_cn_features(cn_prepare$features) -#' } #' @export #' draw_cn_features = function(features, ylab = "", ...) { @@ -243,16 +233,10 @@ draw_cn_features = function(features, ylab = "", ...) { #' @import ggplot2 #' @export #' @examples -#' \donttest{ -#' load(system.file("extdata", "example_cn_list.RData", -#' package = "sigminer", mustWork = TRUE)) -#' segTabs = data.table::rbindlist(tcga_segTabs, idcol = "sample") -#' cn = read_copynumber(segTabs, -#' seg_cols = c("chromosome", "start", "end", "segVal"), -#' genome_build = "hg19") -#' cn_prepare = sig_prepare(cn) +#' # Load copy number prepare object +#' load(system.file("extdata", "toy_copynumber_prepare.RData", +#' package = "sigminer", mustWork = TRUE)) #' draw_cn_components(cn_prepare$features, cn_prepare$components) -#' } #' @family copy number plot #' draw_cn_components = function(features, components, ...) { @@ -376,17 +360,10 @@ draw_cn_components = function(features, components, ...) { #' @import ggplot2 #' @export #' @examples -#' \donttest{ -#' load(system.file("extdata", "example_cn_list.RData", -#' package = "sigminer", mustWork = TRUE)) -#' segTabs = data.table::rbindlist(tcga_segTabs, idcol = "sample") -#' cn = read_copynumber(segTabs, -#' seg_cols = c("chromosome", "start", "end", "segVal"), -#' genome_build = "hg19") -#' cn_prepare = sig_prepare(cn) -#' res = sig_extract(cn_prepare$nmf_matrix, 3, mode = "copynumber", nrun = 5) +#' # Load copy number signature +#' load(system.file("extdata", "toy_copynumber_signature.RData", +#' package = "sigminer", mustWork = TRUE)) #' draw_sig_profile(res$nmfObj) -#' } #' @family signature plot draw_sig_profile = function(nmfObj, mode = c("copynumber", "mutation"), y_scale = c("relative", "absolute"), font_scale = 1) { @@ -481,17 +458,10 @@ draw_sig_profile = function(nmfObj, mode = c("copynumber", "mutation"), #' @importFrom grDevices rainbow #' @export #' @examples -#' \donttest{ -#' load(system.file("extdata", "example_cn_list.RData", -#' package = "sigminer", mustWork = TRUE)) -#' segTabs = data.table::rbindlist(tcga_segTabs, idcol = "sample") -#' cn = read_copynumber(segTabs, -#' seg_cols = c("chromosome", "start", "end", "segVal"), -#' genome_build = "hg19") -#' cn_prepare = sig_prepare(cn) -#' res = sig_extract(cn_prepare$nmf_matrix, 3, mode = "copynumber", nrun = 5) +#' # Load copy number signature +#' load(system.file("extdata", "toy_copynumber_signature.RData", +#' package = "sigminer", mustWork = TRUE)) #' draw_sig_activity(res$nmfObj) -#' } #' @family signature plot draw_sig_activity = function(nmfObj, mode = c("copynumber", "mutation"), font_scale = 1, hide_samps = TRUE) { @@ -574,14 +544,9 @@ draw_sig_activity = function(nmfObj, mode = c("copynumber", "mutation"), #' @export #' @examples #' \donttest{ -#' load(system.file("extdata", "example_cn_list.RData", -#' package = "sigminer", mustWork = TRUE)) -#' segTabs = data.table::rbindlist(tcga_segTabs, idcol = "sample") -#' cn = read_copynumber(segTabs, -#' seg_cols = c("chromosome", "start", "end", "segVal"), -#' genome_build = "hg19") -#' cn_prepare = sig_prepare(cn) -#' res = sig_extract(cn_prepare$nmf_matrix, 3, mode = "copynumber", nrun = 5) +#' # Load copy number signature +#' load(system.file("extdata", "toy_copynumber_signature.RData", +#' package = "sigminer", mustWork = TRUE)) #' sig_activity = sig_get_activity(res$nmfObj) #' sig_cor = sig_get_correlation(sig_activity) #' draw_sig_corrplot(sig_cor) @@ -622,23 +587,20 @@ draw_sig_corrplot = function(mat_list, order = "original", type = "lower", #' @import ggplot2 #' @export #' @examples -#' \donttest{ -#' load(system.file("extdata", "example_cn_list.RData", -#' package = "sigminer", mustWork = TRUE)) -#' segTabs = data.table::rbindlist(tcga_segTabs, idcol = "sample") -#' cn = read_copynumber(segTabs, -#' seg_cols = c("chromosome", "start", "end", "segVal"), -#' genome_build = "hg19") -#' cn_prepare = sig_prepare(cn) -#' res = sig_extract(cn_prepare$nmf_matrix, 3, mode = "copynumber", nrun = 5) -#' subtypes = sig_assign_samples(res$nmfObj) +#' # Load copy number signature +#' load(system.file("extdata", "toy_copynumber_signature.RData", +#' package = "sigminer", mustWork = TRUE)) +#' # Assign samples to clusters +#' subtypes = sig_assign_samples(res$nmfObj, type = "samples") +#' #' set.seed(1234) +#' # Add custom groups #' subtypes$new_group = sample(c("1", "2","3", "4"), size = nrow(subtypes), replace = TRUE) +#' # Summarize subtypes #' subtypes.sum = sig_summarize_subtypes(subtypes[, -1], col_subtype = "nmf_subtypes", -#' cols_to_summary = colnames(subtypes[, -1])[-1], -#' type = c("co", "ca"), verbose = TRUE) -#' plot_list = draw_subtypes_comparison(subtypes.sum) -#' } +#' cols_to_summary = colnames(subtypes[, -1])[c(-1,-2)], +#' type = c("co", "ca"), verbose = TRUE) +#' draw_subtypes_comparison(subtypes.sum) #' @family signature plot draw_subtypes_comparison = function(subtype_summary, xlab = "subtype", ylab_co = NA, diff --git a/R/get_series.R b/R/get_series.R index 9ac94952..59463538 100644 --- a/R/get_series.R +++ b/R/get_series.R @@ -35,11 +35,9 @@ #' @return a `list` #' @export #' @examples -#' \donttest{ #' extdata_dir = system.file("extdata", package = "sigminer", mustWork = TRUE) #' cp = read_copynumber(extdata_dir, pattern = "txt", genome_build = "hg19") #' cn_list = get_cnlist(cp) -#' } #' @family internal calculation function series get_cnlist = function(CopyNumber) { @@ -75,12 +73,10 @@ get_cnlist = function(CopyNumber) { #' @import foreach #' @export #' @examples -#' \donttest{ -#' extdata_dir = system.file("extdata", package = "sigminer", mustWork = TRUE) -#' cp = read_copynumber(extdata_dir, pattern = "txt", genome_build = "hg19") -#' cn_list = get_cnlist(cp) +#' # Load copy number list +#' load(system.file("extdata", "toy_cnlist.RData", +#' package = "sigminer", mustWork = TRUE)) #' cn_features = get_features(cn_list, cores = 1) -#' } #' @family internal calculation function series get_features = function(CN_data, @@ -179,10 +175,9 @@ get_features = function(CN_data, #' @export #' @examples #' \donttest{ -#' extdata_dir = system.file("extdata", package = "sigminer", mustWork = TRUE) -#' cp = read_copynumber(extdata_dir, pattern = "txt", genome_build = "hg19") -#' cn_list = get_cnlist(cp) -#' cn_features = get_features(cn_list, cores = 1) +#' # Load copy number features +#' load(system.file("extdata", "toy_cn_features.RData", +#' package = "sigminer", mustWork = TRUE)) #' cn_components = get_components(cn_features) #' } #' @family internal calculation function series @@ -311,14 +306,14 @@ get_components = function(CN_features, #' @importFrom utils data download.file str #' @export #' @examples -#' \donttest{ -#' extdata_dir = system.file("extdata", package = "sigminer", mustWork = TRUE) -#' cp = read_copynumber(extdata_dir, pattern = "txt", genome_build = "hg19") -#' cn_list = get_cnlist(cp) -#' cn_features = get_features(cn_list, cores = 1) -#' cn_components = get_components(cn_features) +#' # Load copy number components +#' load(system.file("extdata", "toy_cn_components.RData", +#' package = "sigminer", mustWork = TRUE)) +#' # Load copy number features +#' load(system.file("extdata", "toy_cn_features.RData", +#' package = "sigminer", mustWork = TRUE)) +#' #' cn_matrix = get_matrix(cn_features, cn_components) -#' } #' @family internal calculation function series get_matrix = function(CN_features, all_components = NULL, @@ -393,12 +388,10 @@ get_matrix = function(CN_features, #' @return a data table #' @export #' @examples -#' \donttest{ -#' extdata_dir = system.file("extdata", package = "sigminer", mustWork = TRUE) -#' cp = read_copynumber(extdata_dir, pattern = "txt", genome_build = "hg19") -#' cn_list = get_cnlist(cp) +#' # Load copy number list +#' load(system.file("extdata", "toy_cnlist.RData", +#' package = "sigminer", mustWork = TRUE)) #' annot = get_LengthFraction(cn_list, seg_cols = c("chromosome", "start", "end", "segVal")) -#' } #' @family internal calculation function series get_LengthFraction = function(CN_data, genome_build = c("hg19", "hg38"), diff --git a/R/prepare_series.R b/R/prepare_series.R index b3ef7b57..ee97d88a 100644 --- a/R/prepare_series.R +++ b/R/prepare_series.R @@ -21,12 +21,9 @@ #' @export #' @examples #' \donttest{ -#' load(system.file("extdata", "example_cn_list.RData", -#' package = "sigminer", mustWork = TRUE)) -#' segTabs = data.table::rbindlist(tcga_segTabs, idcol = "sample") -#' cn = read_copynumber(segTabs, -#' seg_cols = c("chromosome", "start", "end", "segVal"), -#' genome_build = "hg19") +#' # Load copy number object +#' load(system.file("extdata", "toy_copynumber.RData", +#' package = "sigminer", mustWork = TRUE)) #' cn_prepare = prepare_copynumber(cn) #' } #' @family signature analysis prepare function series diff --git a/R/read_series.R b/R/read_series.R index 99f911ce..389baf3d 100644 --- a/R/read_series.R +++ b/R/read_series.R @@ -12,7 +12,8 @@ #' laml.maf = system.file("extdata", "tcga_laml.maf.gz", package = "maftools") #' laml = read_maf(maf = laml.maf) #' } -#' +#' @export +#' @family read genomic variation data function series read_maf = function( maf, clinicalData = NULL, removeDuplicatedVariants = TRUE, @@ -68,14 +69,12 @@ read_maf = function( #' @return a [CopyNumber] object #' @export #' @examples -#' \donttest{ -#' load(system.file("extdata", "example_cn_list.RData", +#' # Load toy dataset of absolute copynumber profile +#' load(system.file("extdata", "toy_segTab.RData", #' package = "sigminer", mustWork = TRUE)) -#' segTabs = data.table::rbindlist(tcga_segTabs, idcol = "sample") #' cn = read_copynumber(segTabs, -#' seg_cols = c("chromosome", "start", "end", "segVal"), -#' genome_build = "hg19") -#' } +#' seg_cols = c("chromosome", "start", "end", "segVal"), +#' genome_build = "hg19", complement = FALSE, verbose = TRUE) #' @family read genomic variation data function series read_copynumber = function(input, pattern = NULL, @@ -339,6 +338,9 @@ read_copynumber = function(input, message(" Filter - ", nrow(dropoff_df)) } + # make sure seg value is integer + data_df[["segVal"]] = as.integer(round(data_df[["segVal"]])) + if (verbose) message("Anotating...") annot = get_LengthFraction(data_df, genome_build = genome_build, @@ -350,27 +352,16 @@ read_copynumber = function(input, genome_measure = genome_measure ) if (verbose) message("Done!") - if (is.null(clinical_data)) { - res = CopyNumber( - data = data_df, - summary.per.sample = sum_sample, - genome_build = genome_build, - genome_measure = genome_measure, - annotation = annot, - dropoff.segs = dropoff_df, - clinical.data = data.table::data.table() - ) - } else { - res = CopyNumber( - data = data_df, - summary.per.sample = sum_sample, - genome_build = genome_build, - genome_measure = genome_measure, - annotation = annot, - dropoff.segs = dropoff_df, - clinical.data = data.table::as.data.table(clinical_data) - ) - } + + res = CopyNumber( + data = data_df, + summary.per.sample = sum_sample, + genome_build = genome_build, + genome_measure = genome_measure, + annotation = annot, + dropoff.segs = dropoff_df, + clinical.data = data.table::as.data.table(clinical_data) + ) res = validate_segTab(res, verbose = verbose) res @@ -415,20 +406,14 @@ validate_segTab = function(object, verbose = FALSE){ #' @return a [GenomicVariation] object #' @export #' @examples -#' \donttest{ #' # Read MAF #' laml.maf = system.file("extdata", "tcga_laml.maf.gz", package = "maftools") #' laml = read_maf(maf = laml.maf) -#' # Read absolute copy number profile -#' load(system.file("extdata", "example_cn_list.RData", +#' # Load copy number object +#' load(system.file("extdata", "toy_copynumber.RData", #' package = "sigminer", mustWork = TRUE)) -#' segTabs = data.table::rbindlist(tcga_segTabs, idcol = "sample") -#' cn = read_copynumber(segTabs, -#' seg_cols = c("chromosome", "start", "end", "segVal"), -#' genome_build = "hg19") #' # Combine as GenomicVariation object #' gv = read_variation(cn, laml) -#' } #' @family read genomic variation data function series read_variation = function(copynumber, maf, clinical_data = NULL) { diff --git a/R/signature_series.R b/R/signature_series.R index 02b1d6de..ff5b275d 100644 --- a/R/signature_series.R +++ b/R/signature_series.R @@ -5,25 +5,25 @@ # S3 method for signature analysis prepare -------------------------------- -#' Variation signature analysis prepare generic +#' Prepare variation signature analysis #' #' Generate a matrix for NMF de-composition. #' #' The result matrix generated further need to transpose before calling NMF #' if user use [NMF::nmf] by hand. -#' @param object a [CopyNumber] object or [MAF] object or [GenomicVariation] object. -#' @param ... custom setting for operating object. -#' @return a `matrix` used for NMF de-composition. +#' @param object a [CopyNumber] object or [MAF] object or +#' [GenomicVariation] (not support for now) object. +#' @param ... custom setting for operating object. Detail see S3 method for +#' corresponding class. +#' @return a `list` contains a `matrix` used for NMF de-composition. #' @author Shixiang Wang #' @export #' @examples #' \donttest{ -#' load(system.file("extdata", "example_cn_list.RData", -#' package = "sigminer", mustWork = TRUE)) -#' segTabs = data.table::rbindlist(tcga_segTabs, idcol = "sample") -#' cn = read_copynumber(segTabs, -#' seg_cols = c("chromosome", "start", "end", "segVal"), -#' genome_build = "hg19") +#' # Load copy number object +#' load(system.file("extdata", "toy_copynumber.RData", +#' package = "sigminer", mustWork = TRUE)) +#' # Prepare copy number signature analysis #' cn_prepare = sig_prepare(cn) #' } #' @family signature analysis series function @@ -55,10 +55,10 @@ sig_prepare.MAF = function(object, ref_genome = NULL, prefix = NULL, add = add, ignoreChr = ignoreChr, useSyn = useSyn, fn = NULL) } -# # sig_prepare Signature analysis prepare for GenomicVariation object -# sig_prepare.GenomicVariation = function(object) { -# print("Not support right now.") -# } +#' @describeIn sig_prepare Signature analysis prepare for GenomicVariation object +sig_prepare.GenomicVariation = function(object) { + print("Not support right now.") +} @@ -94,13 +94,9 @@ sig_prepare.MAF = function(object, ref_genome = NULL, prefix = NULL, #' @export #' @examples #' \donttest{ -#' load(system.file("extdata", "example_cn_list.RData", -#' package = "sigminer", mustWork = TRUE)) -#' segTabs = data.table::rbindlist(tcga_segTabs, idcol = "sample") -#' cn = read_copynumber(segTabs, -#' seg_cols = c("chromosome", "start", "end", "segVal"), -#' genome_build = "hg19") -#' cn_prepare = sig_prepare(cn) +#' # Load copy number prepare object +#' load(system.file("extdata", "toy_copynumber_prepare.RData", +#' package = "sigminer", mustWork = TRUE)) #' library(NMF) #' cn_estimate = sig_estimate(cn_prepare$nmf_matrix, cores = 1, nrun = 5, #' verbose = TRUE) @@ -293,14 +289,11 @@ sig_estimate <- #' @export #' @examples #' \donttest{ -#' load(system.file("extdata", "example_cn_list.RData", -#' package = "sigminer", mustWork = TRUE)) -#' segTabs = data.table::rbindlist(tcga_segTabs, idcol = "sample") -#' cn = read_copynumber(segTabs, -#' seg_cols = c("chromosome", "start", "end", "segVal"), -#' genome_build = "hg19") -#' cn_prepare = sig_prepare(cn) -#' res = sig_extract(cn_prepare$nmf_matrix, 3, mode = "copynumber", nrun = 5) +#' # Load copy number prepare object +#' load(system.file("extdata", "toy_copynumber_prepare.RData", +#' package = "sigminer", mustWork = TRUE)) +#' # Extract copy number signatures +#' res = sig_extract(cn_prepare$nmf_matrix, 2, mode = "copynumber", nrun = 1) #' } #' @family signature analysis series function sig_extract = function(nmf_matrix, @@ -506,23 +499,18 @@ sig_extract = function(nmf_matrix, #' More detail please see [NMF::predict()]. #' @param nmfObj a `NMF` result object which is an element return from [sig_extract] #' or run results of **NMF** package. -#' @param type cluster type, could be 'consensus' or 'sample'. -#' @param matchConseOrder if `TRUE`, the result will match order as shown in consensus map. -#' @return a `data.frame` +#' @param type cluster type, could be 'consensus' or 'samples'. +#' @param matchConseOrder if `TRUE`, the result will match order as shown in consensus map +#' when type argument is 'consensus'. +#' @return a `data.table` object #' @import NMF cluster #' @export #' @examples -#' \donttest{ -#' load(system.file("extdata", "example_cn_list.RData", -#' package = "sigminer", mustWork = TRUE)) -#' segTabs = data.table::rbindlist(tcga_segTabs, idcol = "sample") -#' cn = read_copynumber(segTabs, -#' seg_cols = c("chromosome", "start", "end", "segVal"), -#' genome_build = "hg19") -#' cn_prepare = sig_prepare(cn) -#' res = sig_extract(cn_prepare$nmf_matrix, 3, mode = "copynumber", nrun = 5) -#' subtypes = sig_assign_samples(res$nmfObj) -#' } +#' # Load copy number signature +#' load(system.file("extdata", "toy_copynumber_signature.RData", +#' package = "sigminer", mustWork = TRUE)) +#' # Assign samples to clusters +#' subtypes = sig_assign_samples(res$nmfObj, type = "samples") #' @family signature analysis series function sig_assign_samples = function(nmfObj, type="consensus", matchConseOrder=F){ @@ -573,17 +561,11 @@ sig_assign_samples = function(nmfObj, type="consensus", matchConseOrder=F){ #' @return a `list` #' @export #' @examples -#' \donttest{ -#' load(system.file("extdata", "example_cn_list.RData", -#' package = "sigminer", mustWork = TRUE)) -#' segTabs = data.table::rbindlist(tcga_segTabs, idcol = "sample") -#' cn = read_copynumber(segTabs, -#' seg_cols = c("chromosome", "start", "end", "segVal"), -#' genome_build = "hg19") -#' cn_prepare = sig_prepare(cn) -#' res = sig_extract(cn_prepare$nmf_matrix, 3, mode = "copynumber", nrun = 5) +#' # Load copy number signature +#' load(system.file("extdata", "toy_copynumber_signature.RData", +#' package = "sigminer", mustWork = TRUE)) +#' # Get activity of signatures #' sig_activity = sig_get_activity(res$nmfObj) -#' } #' @family signature analysis series function sig_get_activity = function(nmfObj) { @@ -628,18 +610,13 @@ sig_get_activity = function(nmfObj) { #' @author Shixiang Wang #' @export #' @examples -#' \donttest{ -#' load(system.file("extdata", "example_cn_list.RData", -#' package = "sigminer", mustWork = TRUE)) -#' segTabs = data.table::rbindlist(tcga_segTabs, idcol = "sample") -#' cn = read_copynumber(segTabs, -#' seg_cols = c("chromosome", "start", "end", "segVal"), -#' genome_build = "hg19") -#' cn_prepare = sig_prepare(cn) -#' res = sig_extract(cn_prepare$nmf_matrix, 3, mode = "copynumber", nrun = 5) +#' # Load copy number signature +#' load(system.file("extdata", "toy_copynumber_signature.RData", +#' package = "sigminer", mustWork = TRUE)) +#' # Get activity of signatures #' sig_activity = sig_get_activity(res$nmfObj) +#' # Get correlation matrix between signature activities #' sig_cor = sig_get_correlation(sig_activity) -#' } #' @family signature analysis series function sig_get_correlation = function(cn_activity=NULL, snv_activity=NULL, type = c("absolute", "relative"), @@ -770,22 +747,19 @@ sig_get_similarity = function(sig1, sig2, type = c("cos", "cor")) { #' @return a `list` contains data, summary, p value etc.. #' @export #' @examples -#' \donttest{ -#' load(system.file("extdata", "example_cn_list.RData", -#' package = "sigminer", mustWork = TRUE)) -#' segTabs = data.table::rbindlist(tcga_segTabs, idcol = "sample") -#' cn = read_copynumber(segTabs, -#' seg_cols = c("chromosome", "start", "end", "segVal"), -#' genome_build = "hg19") -#' cn_prepare = sig_prepare(cn) -#' res = sig_extract(cn_prepare$nmf_matrix, 3, mode = "copynumber", nrun = 5) -#' subtypes = sig_assign_samples(res$nmfObj) +#' # Load copy number signature +#' load(system.file("extdata", "toy_copynumber_signature.RData", +#' package = "sigminer", mustWork = TRUE)) +#' # Assign samples to clusters +#' subtypes = sig_assign_samples(res$nmfObj, type = "samples") +#' #' set.seed(1234) +#' # Add custom groups #' subtypes$new_group = sample(c("1", "2","3", "4"), size = nrow(subtypes), replace = TRUE) +#' # Summarize subtypes #' subtypes.sum = sig_summarize_subtypes(subtypes[, -1], col_subtype = "nmf_subtypes", -#' cols_to_summary = colnames(subtypes[, -1])[-1], -#' type = c("co", "ca"), verbose = TRUE) -#' } +#' cols_to_summary = colnames(subtypes[, -1])[c(-1,-2)], +#' type = c("co", "ca"), verbose = TRUE) #' @family signature analysis series function sig_summarize_subtypes = function(data, col_subtype, cols_to_summary, type = "ca", verbose = FALSE){ diff --git a/README.Rmd b/README.Rmd index 7c9304c8..c4641838 100644 --- a/README.Rmd +++ b/README.Rmd @@ -14,9 +14,7 @@ knitr::opts_chunk$set( ``` -# sigminer - - +# sigminer [![CRAN status](https://www.r-pkg.org/badges/version/sigminer)](https://cran.r-project.org/package=sigminer) [![lifecycle](https://img.shields.io/badge/lifecycle-maturing-blue.svg)](https://www.tidyverse.org/lifecycle/#maturing) [![AppVeyor build status](https://ci.appveyor.com/api/projects/status/github/ShixiangWang/sigminer?branch=master&svg=true)](https://ci.appveyor.com/project/ShixiangWang/sigminer) [![Travis build status](https://travis-ci.org/ShixiangWang/sigminer.svg?branch=master)](https://travis-ci.org/ShixiangWang/sigminer) [![Coverage status](https://codecov.io/gh/ShixiangWang/sigminer/branch/master/graph/badge.svg)](https://codecov.io/github/ShixiangWang/sigminer?branch=master) @@ -29,7 +27,7 @@ The goal of **sigminer** is to provide an uniform interface for genomic variatio You can install the development version of sigminer from Github with: ```{r, eval=FALSE} -devtools::install_github("ShixiangWang/sigminer") +remotes::install_github("ShixiangWang/sigminer") ``` diff --git a/README.md b/README.md index b1b05f80..0bef8fd3 100644 --- a/README.md +++ b/README.md @@ -1,9 +1,7 @@ -# sigminer - - +# sigminer [![CRAN status](https://www.r-pkg.org/badges/version/sigminer)](https://cran.r-project.org/package=sigminer) @@ -32,15 +30,15 @@ and [maftools](https://github.com/PoisonAlien/maftools) package. You can install the development version of sigminer from Github with: ``` r -devtools::install_github("ShixiangWang/sigminer") +remotes::install_github("ShixiangWang/sigminer") ``` ## Citation - - *Macintyre, Geoff, et al. “Copy number signatures and mutational + - *Macintyre, Geoff, et al. “Copy number signatures and mutational processes in ovarian carcinoma.” Nature genetics 50.9 (2018): 1262.* - - *Wang, Shixiang, et al. “APOBEC3B and APOBEC mutational signature as + - *Wang, Shixiang, et al. “APOBEC3B and APOBEC mutational signature as potential predictive markers for immunotherapy response in non-small cell lung cancer.” Oncogene (2018).* diff --git a/inst/extdata/toy_cn.RData b/inst/extdata/toy_cn.RData new file mode 100644 index 0000000000000000000000000000000000000000..e435a93edb44b2f5ebc227260093ca304d57d7d5 GIT binary patch literal 11059 zcmbVQRZtwzmJGo?KyY_=8JqyYEx5b8Td?3HxVr^+8{FM3xclHtU~m~WZ)<hbWTQiV&L`|GPsFkF@-v&QA7s4+QW zhex$|6ctM&`1JMO@o5wSj#@P&rI 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copy number } \examples{ -\donttest{ -extdata_dir = system.file("extdata", package = "sigminer", mustWork = TRUE) -cp = read_copynumber(extdata_dir, pattern = "txt", genome_build = "hg19") -cn_list = get_cnlist(cp) +# Load copy number list +load(system.file("extdata", "toy_cnlist.RData", + package = "sigminer", mustWork = TRUE)) annot = get_LengthFraction(cn_list, seg_cols = c("chromosome", "start", "end", "segVal")) } -} \seealso{ Other internal calculation function series: \code{\link{get_ArmLocation}}, \code{\link{get_cnlist}}, diff --git a/man/get_cnlist.Rd b/man/get_cnlist.Rd index 41774f52..e903347e 100644 --- a/man/get_cnlist.Rd +++ b/man/get_cnlist.Rd @@ -16,12 +16,10 @@ a \code{list} Extract copy number profile as list from CopyNumber object } \examples{ -\donttest{ extdata_dir = system.file("extdata", package = "sigminer", mustWork = TRUE) cp = read_copynumber(extdata_dir, pattern = "txt", genome_build = "hg19") cn_list = get_cnlist(cp) } -} \seealso{ Other internal calculation function series: \code{\link{get_ArmLocation}}, \code{\link{get_LengthFraction}}, diff --git a/man/get_components.Rd b/man/get_components.Rd index d5322e5c..f280deae 100644 --- a/man/get_components.Rd +++ b/man/get_components.Rd @@ -37,10 +37,9 @@ of Gaussian or mixtures of Poison distributions using \strong{flexmix} package. } \examples{ \donttest{ -extdata_dir = system.file("extdata", package = "sigminer", mustWork = TRUE) -cp = read_copynumber(extdata_dir, pattern = "txt", genome_build = "hg19") -cn_list = get_cnlist(cp) -cn_features = get_features(cn_list, cores = 1) +# Load copy number features +load(system.file("extdata", "toy_cn_features.RData", + package = "sigminer", mustWork = TRUE)) cn_components = get_components(cn_features) } } diff --git a/man/get_features.Rd b/man/get_features.Rd index 82fc02c7..16493d9a 100644 --- a/man/get_features.Rd +++ b/man/get_features.Rd @@ -27,13 +27,11 @@ segment copy-number, breakpoint number (per chromosome arm), length of segments copy-number. } \examples{ -\donttest{ -extdata_dir = system.file("extdata", package = "sigminer", mustWork = TRUE) -cp = read_copynumber(extdata_dir, pattern = "txt", genome_build = "hg19") -cn_list = get_cnlist(cp) +# Load copy number list +load(system.file("extdata", "toy_cnlist.RData", + package = "sigminer", mustWork = TRUE)) cn_features = get_features(cn_list, cores = 1) } -} \seealso{ Other internal calculation function series: \code{\link{get_ArmLocation}}, \code{\link{get_LengthFraction}}, diff --git a/man/get_matrix.Rd b/man/get_matrix.Rd index d65b28e2..af5cf205 100644 --- a/man/get_matrix.Rd +++ b/man/get_matrix.Rd @@ -29,15 +29,15 @@ Generate a sample-by-component matrix representing the sum of posterior probabilities of each copy-number event being assigned to each component. } \examples{ -\donttest{ -extdata_dir = system.file("extdata", package = "sigminer", mustWork = TRUE) -cp = read_copynumber(extdata_dir, pattern = "txt", genome_build = "hg19") -cn_list = get_cnlist(cp) -cn_features = get_features(cn_list, cores = 1) -cn_components = get_components(cn_features) +# Load copy number components +load(system.file("extdata", "toy_cn_components.RData", + package = "sigminer", mustWork = TRUE)) +# Load copy number features +load(system.file("extdata", "toy_cn_features.RData", + package = "sigminer", mustWork = TRUE)) + cn_matrix = get_matrix(cn_features, cn_components) } -} \seealso{ Other internal calculation function series: \code{\link{get_ArmLocation}}, \code{\link{get_LengthFraction}}, diff --git a/man/prepare_copynumber.Rd b/man/prepare_copynumber.Rd index caab003d..f10a0575 100644 --- a/man/prepare_copynumber.Rd +++ b/man/prepare_copynumber.Rd @@ -49,12 +49,9 @@ Prepare nmf input matrix for copy number signature analysis } \examples{ \donttest{ -load(system.file("extdata", "example_cn_list.RData", - package = "sigminer", mustWork = TRUE)) -segTabs = data.table::rbindlist(tcga_segTabs, idcol = "sample") -cn = read_copynumber(segTabs, - seg_cols = c("chromosome", "start", "end", "segVal"), - genome_build = "hg19") +# Load copy number object +load(system.file("extdata", "toy_copynumber.RData", + package = "sigminer", mustWork = TRUE)) cn_prepare = prepare_copynumber(cn) } } diff --git a/man/read_copynumber.Rd b/man/read_copynumber.Rd index 94af44b0..5d1e5aa9 100644 --- a/man/read_copynumber.Rd +++ b/man/read_copynumber.Rd @@ -55,14 +55,12 @@ Read \strong{absolute} copy number profile for preparing CNV signature analysis. } \examples{ -\donttest{ -load(system.file("extdata", "example_cn_list.RData", +# Load toy dataset of absolute copynumber profile +load(system.file("extdata", "toy_segTab.RData", package = "sigminer", mustWork = TRUE)) -segTabs = data.table::rbindlist(tcga_segTabs, idcol = "sample") cn = read_copynumber(segTabs, - seg_cols = c("chromosome", "start", "end", "segVal"), - genome_build = "hg19") -} + seg_cols = c("chromosome", "start", "end", "segVal"), + genome_build = "hg19", complement = FALSE, verbose = TRUE) } \seealso{ Other read genomic variation data function series: \code{\link{read_maf}}, diff --git a/man/read_maf.Rd b/man/read_maf.Rd index 1cdd0005..6a262628 100644 --- a/man/read_maf.Rd +++ b/man/read_maf.Rd @@ -58,12 +58,14 @@ This note can be ignored without any harm, it's only generated as to make user a laml.maf = system.file("extdata", "tcga_laml.maf.gz", package = "maftools") laml = read_maf(maf = laml.maf) } - } \references{ Shyr C, Tarailo-Graovac M, Gottlieb M, Lee JJ, van Karnebeek C, Wasserman WW. FLAGS, frequently mutated genes in public exomes. BMC Med Genomics 2014; 7: 64. } \seealso{ +Other read genomic variation data function series: \code{\link{read_copynumber}}, + \code{\link{read_variation}} + Other read genomic variation data function series: \code{\link{read_copynumber}}, \code{\link{read_variation}} } diff --git a/man/read_variation.Rd b/man/read_variation.Rd index f2467b52..e29f36af 100644 --- a/man/read_variation.Rd +++ b/man/read_variation.Rd @@ -22,21 +22,15 @@ Read \link{CopyNumber} and \link{MAF} object as a new S4 object \link{GenomicVar for uniform variation analysis. \strong{The function is initialized to construct structure of sigminer, please dont use it for now}. } \examples{ -\donttest{ # Read MAF laml.maf = system.file("extdata", "tcga_laml.maf.gz", package = "maftools") laml = read_maf(maf = laml.maf) -# Read absolute copy number profile -load(system.file("extdata", "example_cn_list.RData", +# Load copy number object +load(system.file("extdata", "toy_copynumber.RData", package = "sigminer", mustWork = TRUE)) -segTabs = data.table::rbindlist(tcga_segTabs, idcol = "sample") -cn = read_copynumber(segTabs, - seg_cols = c("chromosome", "start", "end", "segVal"), - genome_build = "hg19") # Combine as GenomicVariation object gv = read_variation(cn, laml) } -} \seealso{ Other read genomic variation data function series: \code{\link{read_copynumber}}, \code{\link{read_maf}} diff --git a/man/sig_assign_samples.Rd b/man/sig_assign_samples.Rd index c4ecd805..52d8524f 100644 --- a/man/sig_assign_samples.Rd +++ b/man/sig_assign_samples.Rd @@ -10,12 +10,13 @@ sig_assign_samples(nmfObj, type = "consensus", matchConseOrder = F) \item{nmfObj}{a \code{NMF} result object which is an element return from \link{sig_extract} or run results of \strong{NMF} package.} -\item{type}{cluster type, could be 'consensus' or 'sample'.} +\item{type}{cluster type, could be 'consensus' or 'samples'.} -\item{matchConseOrder}{if \code{TRUE}, the result will match order as shown in consensus map.} +\item{matchConseOrder}{if \code{TRUE}, the result will match order as shown in consensus map +when type argument is 'consensus'.} } \value{ -a \code{data.frame} +a \code{data.table} object } \description{ One of key results from NMF decomposition is to cluster samples into different @@ -31,17 +32,11 @@ After NMF run, use this function to select import features and assign groups for More detail please see \code{\link[NMF:predict]{NMF::predict()}}. } \examples{ -\donttest{ -load(system.file("extdata", "example_cn_list.RData", - package = "sigminer", mustWork = TRUE)) -segTabs = data.table::rbindlist(tcga_segTabs, idcol = "sample") -cn = read_copynumber(segTabs, - seg_cols = c("chromosome", "start", "end", "segVal"), - genome_build = "hg19") -cn_prepare = sig_prepare(cn) -res = sig_extract(cn_prepare$nmf_matrix, 3, mode = "copynumber", nrun = 5) -subtypes = sig_assign_samples(res$nmfObj) -} +# Load copy number signature +load(system.file("extdata", "toy_copynumber_signature.RData", + package = "sigminer", mustWork = TRUE)) +# Assign samples to clusters +subtypes = sig_assign_samples(res$nmfObj, type = "samples") } \seealso{ Other signature analysis series function: \code{\link{sig_estimate}}, diff --git a/man/sig_estimate.Rd b/man/sig_estimate.Rd index c5984209..7bd78797 100644 --- a/man/sig_estimate.Rd +++ b/man/sig_estimate.Rd @@ -51,13 +51,9 @@ More custom features please directly use \link[NMF:nmfEstimateRank]{NMF::nmfEsti } \examples{ \donttest{ -load(system.file("extdata", "example_cn_list.RData", - package = "sigminer", mustWork = TRUE)) -segTabs = data.table::rbindlist(tcga_segTabs, idcol = "sample") -cn = read_copynumber(segTabs, - seg_cols = c("chromosome", "start", "end", "segVal"), - genome_build = "hg19") -cn_prepare = sig_prepare(cn) +# Load copy number prepare object +load(system.file("extdata", "toy_copynumber_prepare.RData", + package = "sigminer", mustWork = TRUE)) library(NMF) cn_estimate = sig_estimate(cn_prepare$nmf_matrix, cores = 1, nrun = 5, verbose = TRUE) diff --git a/man/sig_extract.Rd b/man/sig_extract.Rd index 9525e608..17986fe4 100644 --- a/man/sig_extract.Rd +++ b/man/sig_extract.Rd @@ -35,14 +35,11 @@ Do NMF de-composition and then extract signatures. } \examples{ \donttest{ -load(system.file("extdata", "example_cn_list.RData", - package = "sigminer", mustWork = TRUE)) -segTabs = data.table::rbindlist(tcga_segTabs, idcol = "sample") -cn = read_copynumber(segTabs, - seg_cols = c("chromosome", "start", "end", "segVal"), - genome_build = "hg19") -cn_prepare = sig_prepare(cn) -res = sig_extract(cn_prepare$nmf_matrix, 3, mode = "copynumber", nrun = 5) +# Load copy number prepare object +load(system.file("extdata", "toy_copynumber_prepare.RData", + package = "sigminer", mustWork = TRUE)) +# Extract copy number signatures +res = sig_extract(cn_prepare$nmf_matrix, 2, mode = "copynumber", nrun = 1) } } \seealso{ diff --git a/man/sig_get_activity.Rd b/man/sig_get_activity.Rd index 34b326e3..2fa6283d 100644 --- a/man/sig_get_activity.Rd +++ b/man/sig_get_activity.Rd @@ -17,18 +17,12 @@ a \code{list} Get signature activity } \examples{ -\donttest{ -load(system.file("extdata", "example_cn_list.RData", - package = "sigminer", mustWork = TRUE)) -segTabs = data.table::rbindlist(tcga_segTabs, idcol = "sample") -cn = read_copynumber(segTabs, - seg_cols = c("chromosome", "start", "end", "segVal"), - genome_build = "hg19") -cn_prepare = sig_prepare(cn) -res = sig_extract(cn_prepare$nmf_matrix, 3, mode = "copynumber", nrun = 5) +# Load copy number signature +load(system.file("extdata", "toy_copynumber_signature.RData", + package = "sigminer", mustWork = TRUE)) +# Get activity of signatures sig_activity = sig_get_activity(res$nmfObj) } -} \seealso{ Other signature analysis series function: \code{\link{sig_assign_samples}}, \code{\link{sig_estimate}}, \code{\link{sig_extract}}, diff --git a/man/sig_get_correlation.Rd b/man/sig_get_correlation.Rd index f837edba..1a2920b9 100644 --- a/man/sig_get_correlation.Rd +++ b/man/sig_get_correlation.Rd @@ -25,19 +25,14 @@ a \code{list}. Compute correlation matrix and corresponding statistical test values. } \examples{ -\donttest{ -load(system.file("extdata", "example_cn_list.RData", - package = "sigminer", mustWork = TRUE)) -segTabs = data.table::rbindlist(tcga_segTabs, idcol = "sample") -cn = read_copynumber(segTabs, - seg_cols = c("chromosome", "start", "end", "segVal"), - genome_build = "hg19") -cn_prepare = sig_prepare(cn) -res = sig_extract(cn_prepare$nmf_matrix, 3, mode = "copynumber", nrun = 5) +# Load copy number signature +load(system.file("extdata", "toy_copynumber_signature.RData", + package = "sigminer", mustWork = TRUE)) +# Get activity of signatures sig_activity = sig_get_activity(res$nmfObj) +# Get correlation matrix between signature activities sig_cor = sig_get_correlation(sig_activity) } -} \seealso{ Other signature analysis series function: \code{\link{sig_assign_samples}}, \code{\link{sig_estimate}}, \code{\link{sig_extract}}, diff --git a/man/sig_prepare.Rd b/man/sig_prepare.Rd index 4a0f30ac..74c16752 100644 --- a/man/sig_prepare.Rd +++ b/man/sig_prepare.Rd @@ -4,7 +4,8 @@ \alias{sig_prepare} \alias{sig_prepare.CopyNumber} \alias{sig_prepare.MAF} -\title{Variation signature analysis prepare generic} +\alias{sig_prepare.GenomicVariation} +\title{Prepare variation signature analysis} \usage{ sig_prepare(object, ...) @@ -15,11 +16,15 @@ sig_prepare(object, ...) \method{sig_prepare}{MAF}(object, ref_genome = NULL, prefix = NULL, add = TRUE, ignoreChr = NULL, useSyn = TRUE) + +\method{sig_prepare}{GenomicVariation}(object) } \arguments{ -\item{object}{a \link{CopyNumber} object or \link{MAF} object or \link{GenomicVariation} object.} +\item{object}{a \link{CopyNumber} object or \link{MAF} object or +\link{GenomicVariation} (not support for now) object.} -\item{...}{custom setting for operating object.} +\item{...}{custom setting for operating object. Detail see S3 method for +corresponding class.} \item{reference_components}{default is \code{FALSE}, calculate mixture components from \link{CopyNumber} object. @@ -63,7 +68,7 @@ Default NULL, tries to auto-detect from installed genomes.} \item{useSyn}{Logical. Whether to include synonymous variants in analysis. Defaults to TRUE} } \value{ -a \code{matrix} used for NMF de-composition. +a \code{list} contains a \code{matrix} used for NMF de-composition. } \description{ Generate a matrix for NMF de-composition. @@ -77,16 +82,16 @@ if user use \link[NMF:nmf]{NMF::nmf} by hand. \item \code{CopyNumber}: Signature analysis prepare for CopyNumber object \item \code{MAF}: Signature analysis prepare for CopyNumber object + +\item \code{GenomicVariation}: Signature analysis prepare for GenomicVariation object }} \examples{ \donttest{ -load(system.file("extdata", "example_cn_list.RData", - package = "sigminer", mustWork = TRUE)) -segTabs = data.table::rbindlist(tcga_segTabs, idcol = "sample") -cn = read_copynumber(segTabs, - seg_cols = c("chromosome", "start", "end", "segVal"), - genome_build = "hg19") +# Load copy number object +load(system.file("extdata", "toy_copynumber.RData", + package = "sigminer", mustWork = TRUE)) +# Prepare copy number signature analysis cn_prepare = sig_prepare(cn) } } diff --git a/man/sig_summarize_subtypes.Rd b/man/sig_summarize_subtypes.Rd index a23c023b..b8c0a5c9 100644 --- a/man/sig_summarize_subtypes.Rd +++ b/man/sig_summarize_subtypes.Rd @@ -32,22 +32,19 @@ summary table and Tukey Honest significant difference (using \link[stats:TukeyHS The result of this function can be plotted by \code{\link[=draw_subtypes_comparison]{draw_subtypes_comparison()}}. } \examples{ -\donttest{ -load(system.file("extdata", "example_cn_list.RData", - package = "sigminer", mustWork = TRUE)) -segTabs = data.table::rbindlist(tcga_segTabs, idcol = "sample") -cn = read_copynumber(segTabs, - seg_cols = c("chromosome", "start", "end", "segVal"), - genome_build = "hg19") -cn_prepare = sig_prepare(cn) -res = sig_extract(cn_prepare$nmf_matrix, 3, mode = "copynumber", nrun = 5) -subtypes = sig_assign_samples(res$nmfObj) +# Load copy number signature +load(system.file("extdata", "toy_copynumber_signature.RData", + package = "sigminer", mustWork = TRUE)) +# Assign samples to clusters +subtypes = sig_assign_samples(res$nmfObj, type = "samples") + set.seed(1234) +# Add custom groups subtypes$new_group = sample(c("1", "2","3", "4"), size = nrow(subtypes), replace = TRUE) +# Summarize subtypes subtypes.sum = sig_summarize_subtypes(subtypes[, -1], col_subtype = "nmf_subtypes", - cols_to_summary = colnames(subtypes[, -1])[-1], - type = c("co", "ca"), verbose = TRUE) -} + cols_to_summary = colnames(subtypes[, -1])[c(-1,-2)], + type = c("co", "ca"), verbose = TRUE) } \seealso{ Other signature analysis series function: \code{\link{sig_assign_samples}},