diff --git a/.github/workflows/windows-build.yml b/.github/workflows/windows-build.yml
new file mode 100644
index 000000000..8fd9684bd
--- /dev/null
+++ b/.github/workflows/windows-build.yml
@@ -0,0 +1,38 @@
+name: Windows/MinGW-W64 CI
+on: [push, pull_request]
+
+jobs:
+  build:
+    runs-on: windows-latest
+    steps:
+    - name: Checkout
+      uses: actions/checkout@v4
+    - name: Set up MSYS2 MinGW-W64
+      uses: msys2/setup-msys2@v2
+      with:
+        msystem: mingw64
+        update: false
+        install: >-
+          git
+          autotools
+          gcc
+          zlib-devel
+          libbz2-devel
+          liblzma-devel
+          ncurses-devel
+          mingw-w64-x86_64-tools-git
+    - name: Compile htslib
+      shell: msys2 {0}
+      run: |
+        export PATH=$PATH:/mingw64/bin
+        export MSYSTEM=MINGW64
+        autoreconf -i
+        ./configure
+        make -j32
+    - name: Check Htslib
+      shell: msys2 {0}
+      run: |
+        export PATH=$PATH:/mingw64/bin
+        export MSYSTEM=MINGW64
+        make check
+