diff --git a/lib/galaxy/tool_util/deps/conda_util.py b/lib/galaxy/tool_util/deps/conda_util.py
index d5190383a1f9..1c688cdd7c0f 100644
--- a/lib/galaxy/tool_util/deps/conda_util.py
+++ b/lib/galaxy/tool_util/deps/conda_util.py
@@ -48,7 +48,7 @@
 VERSIONED_ENV_DIR_NAME = re.compile(r"__(.*)@(.*)")
 UNVERSIONED_ENV_DIR_NAME = re.compile(r"__(.*)@_uv_")
 USE_PATH_EXEC_DEFAULT = False
-CONDA_PACKAGE_SPECS = ("conda>=23.7.0", "conda-libmamba-solver", "'pyopenssl>=22.1.0'")
+CONDA_PACKAGE_SPECS = ("conda>=23.7.0", "conda-libmamba-solver", "pyopenssl>=22.1.0")
 CONDA_BUILD_SPECS = ("conda-build>=3.22.0",)
 USE_LOCAL_DEFAULT = False