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CHANGELOG.md

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nf-core/mag: Changelog

The format is based on Keep a Changelog and this project adheres to Semantic Versioning.

v1.1.0 - 2020/10/06

Added

  • #35 - Add social preview image
  • #49 - Add host read removal with Bowtie 2 and according custom section to MultiQC
  • #49 - Add separate MultiQC section for FastQC after preprocessing
  • #65 - Add MetaBAT2 RNG seed parameter --metabat_rng_seed and set the default to 1 which ensures reproducible binning results
  • #65 - Add parameters --megahit_fix_cpu_1, --spades_fix_cpus and --spadeshybrid_fix_cpus to ensure reproducible results from assembly tools
  • #66 - Export depth.txt.gz into result folder
  • #67 - Compress assembly files
  • #82 - Add nextflow_schema.json
  • #104 - Add parameter --save_busco_reference

Changed

  • #56 - Update MetaBAT2 from v2.13 to v2.15
  • #46 - Update MultiQC from v1.7 to v1.9
  • #88 - Update to new nf-core 1.10.2 TEMPLATE
  • #88 - --reads is now removed, use --input instead
  • #101 - Prevented PhiX alignments from being stored in work directory #97
  • #104, #111 - Update BUSCO from v3.0.2 to v4.1.4

Fixed

  • #29 - Fix MetaBAT2 binning discards unbinned contigs #27
  • #31, #36, #76, #107 - Fix links in README
  • #47 - Fix missing MultiQC when --skip_quast or --skip_busco was specified
  • #49, #89 - Added missing parameters to summary
  • #50 - Fix missing channels when --keep_phix is specified
  • #54 - Updated links to minikraken db
  • #54 - Fixed Kraken2 dp preparation: allow different names for compressed archive file and contained folder as for some minikraken dbs
  • #55 - Fixed channel joining for multiple samples causing MetaBAT2 error #32
  • #57 - Fix number of threads used by MetaBAT2 program jgi_summarize_bam_contig_depths
  • #70 - Fix SPAdes memory conversion issue #61
  • #71 - No more ignoring errors in SPAdes assembly
  • #72 - No more ignoring of BUSCO errors
  • #73, #75 - Improved output documentation
  • #96 - Fix missing bin names in MultiQC BUSCO section #78
  • #104 - Fix BUSCO errors causing missing summary output #77

Deprecated

  • #29 - Change depreciated parameters: --singleEnd -> --single_end, --igenomesIgnore -> --igenomes_ignore

v1.0.0 - 2019/12/20

Initial release of nf-core/mag, created with the nf-core template.

Added

  • short and long reads QC (fastp, porechop, filtlong, fastqc)
  • Lambda and PhiX detection and filtering (bowtie2, nanolyse)
  • Taxonomic classification of reads (centrifuge, kraken2)
  • Short read and hybrid assembly (megahit, metaspades)
  • metagenome binning (metabat2)
  • QC of bins (busco, quast)
  • annotation (cat/bat)