From eae49149c9b2c78a80a08d9040df0bd8ecda26a6 Mon Sep 17 00:00:00 2001 From: Marina Gourtovaia Date: Thu, 30 Apr 2020 09:16:33 +0100 Subject: [PATCH 1/4] disable download of *.fa files from staging folder view --- Changes | 3 +++ wtsi_local/httpd_sfweb.conf | 2 +- 2 files changed, 4 insertions(+), 1 deletion(-) diff --git a/Changes b/Changes index 78f50cf6..d21b0e52 100644 --- a/Changes +++ b/Changes @@ -1,5 +1,8 @@ LIST OF CHANGES + - disable downloading *.fa (consensus) files from tracking server + views of staging folders + release 91.3.0 - pp_archive_path runfolder accessor - a path to an archive directory for third party portable pipelines diff --git a/wtsi_local/httpd_sfweb.conf b/wtsi_local/httpd_sfweb.conf index d85839ab..36d30eac 100644 --- a/wtsi_local/httpd_sfweb.conf +++ b/wtsi_local/httpd_sfweb.conf @@ -26,7 +26,7 @@ Alias "/export" "/export" # Disable access to (download of) some files. # Does not prevent them being listed in the directory view above. # - + Require all denied From 551eebd0f9cdf3c15be999b14354d04ac924da65 Mon Sep 17 00:00:00 2001 From: jmtcsngr Date: Thu, 4 Jun 2020 11:30:36 +0100 Subject: [PATCH 2/4] add npg samplesheet executable --- Changes | 1 + MANIFEST | 1 + bin/npg_samplesheet4MiSeq | 92 +++++++++++++++++++++++++++++++++++++++ 3 files changed, 94 insertions(+) create mode 100755 bin/npg_samplesheet4MiSeq diff --git a/Changes b/Changes index d21b0e52..6c1158f5 100644 --- a/Changes +++ b/Changes @@ -2,6 +2,7 @@ LIST OF CHANGES - disable downloading *.fa (consensus) files from tracking server views of staging folders + - add executable for npg samplesheet4MiSeq wrapper release 91.3.0 - pp_archive_path runfolder accessor - a path to an archive directory diff --git a/MANIFEST b/MANIFEST index 333034d4..be53216b 100644 --- a/MANIFEST +++ b/MANIFEST @@ -2,6 +2,7 @@ bin/event_notifications bin/illumina_instruments_uptime bin/npg_daemon_control bin/npg_move_runfolder +bin/npg_samplesheet4MiSeq bin/npg_status2file bin/npg_status_watcher bin/staging_area_monitor diff --git a/bin/npg_samplesheet4MiSeq b/bin/npg_samplesheet4MiSeq new file mode 100755 index 00000000..6cf05cb6 --- /dev/null +++ b/bin/npg_samplesheet4MiSeq @@ -0,0 +1,92 @@ +#!/usr/bin/env perl + +use strict; +use warnings; +use FindBin qw($Bin); +use lib ( -d "$Bin/../lib/perl5" ? "$Bin/../lib/perl5" : "$Bin/../lib" ); +use Log::Log4perl qw(:easy); + +use npg::samplesheet::auto; + +our $VERSION = '0'; + +Log::Log4perl->easy_init($INFO); + +my $log = Log::Log4perl->get_logger('main'); +$log->info("Starting npg samplesheet"); + +npg::samplesheet::auto->new()->loop(); + +exit 0; + +1; + +__END__ + +=head1 NAME + +npg_samplesheet4MiSeq + +=head1 USAGE + + npg_samplesheet4MiSeq + +=head1 DESCRIPTION + + The script, once started, runs in perpetuity, generating Illumina-style + samplesheets for any MiSeq run with status 'run pending'. + +=head1 REQUIRED ARGUMENTS + + None + +=head1 OPTIONS + +=head1 DIAGNOSTICS + +=head1 CONFIGURATION + +=head1 DEPENDENCIES + +=over + +=item strict + +=item warnings + +=item FindBin + +=item lib + +=item Log::Log4perl + +=back + +=head1 INCOMPATIBILITIES + +=head1 BUGS AND LIMITATIONS + +=head1 AUTHOR + +Jaime Tovar Ejmtc@sanger.ac.ukE + +=head1 LICENSE AND COPYRIGHT + +Copyright (C) 2020 Genome Research Limited + +This file is part of NPG. + +NPG is free software: you can redistribute it and/or modify +it under the terms of the GNU General Public License as published by +the Free Software Foundation, either version 3 of the License, or +(at your option) any later version. + +This program is distributed in the hope that it will be useful, +but WITHOUT ANY WARRANTY; without even the implied warranty of +MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the +GNU General Public License for more details. + +You should have received a copy of the GNU General Public License +along with this program. If not, see . + +=cut From d9ce4808aa854148cfa1ec65850737c404746ef3 Mon Sep 17 00:00:00 2001 From: mgcam Date: Mon, 8 Jun 2020 11:19:38 +0100 Subject: [PATCH 3/4] st::api::lims - new sample props (#576) --- Changes | 2 + lib/st/api/lims.pm | 2 + t/40-st-lims-mlwarehouse.t | 21 +- t/40-st-lims.t | 4 +- t/data/fixtures_lims_wh/000-Sample.yml | 6 +- t/data/samplesheet/1control7libs_extended.csv | 20 +- t/data/samplesheet/4pool4libs_extended.csv | 224 +++++++++--------- t/data/samplesheet/6946_extended.csv | 28 +-- t/data/samplesheet/7007_extended.csv | 6 +- t/data/samplesheet/8pools_extended.csv | 140 +++++------ t/data/samplesheet/dual_index_extended.csv | 16 +- t/data/samplesheet/novaseq_multirun.csv | 20 +- 12 files changed, 257 insertions(+), 232 deletions(-) diff --git a/Changes b/Changes index 6c1158f5..640a6ade 100644 --- a/Changes +++ b/Changes @@ -3,6 +3,8 @@ LIST OF CHANGES - disable downloading *.fa (consensus) files from tracking server views of staging folders - add executable for npg samplesheet4MiSeq wrapper + - st::api::lims - two new accessors, 'sample_is_control' + and 'sample_control_type' release 91.3.0 - pp_archive_path runfolder accessor - a path to an archive directory diff --git a/lib/st/api/lims.pm b/lib/st/api/lims.pm index 580d3458..2b621310 100644 --- a/lib/st/api/lims.pm +++ b/lib/st/api/lims.pm @@ -111,6 +111,8 @@ Readonly::Hash my %METHODS_PER_CATEGORY => { sample_supplier_name sample_cohort sample_donor_id + sample_is_control + sample_control_type /], 'study' => [qw/ study_id diff --git a/t/40-st-lims-mlwarehouse.t b/t/40-st-lims-mlwarehouse.t index 2a2a05fc..7d2b3b85 100644 --- a/t/40-st-lims-mlwarehouse.t +++ b/t/40-st-lims-mlwarehouse.t @@ -8,7 +8,7 @@ my $available = eval "require $driver_package"; if (!$available) { plan skip_all => "$driver_package is not deployed or cannot be loaded"; } else { - plan tests => 5; + plan tests => 6; use_ok('st::api::lims'); @@ -144,6 +144,25 @@ if (!$available) { }; + subtest 'sample controls' => sub { + plan tests => 6; + + my $init = {id_flowcell_lims => 57543, position => 1, + driver_type => 'ml_warehouse', mlwh_schema => $schema_wh}; + + for my $ti ((1,2,3)) { + $init->{tag_index} = $ti; + my $l = st::api::lims->new($init); + if ($ti <= 2 ) { + ok($l->sample_is_control, 'sample is control'); + my $ctype = ($ti == 1) ? 'negative' : 'positive'; + is($l->sample_control_type, $ctype, 'correct control type'); + } else { + ok(!$l->sample_is_control, 'sample is not control'); + is($l->sample_control_type, undef, 'sample control type is undefined'); + } + } + }; } 1; diff --git a/t/40-st-lims.t b/t/40-st-lims.t index a6192fa0..faeae8b5 100644 --- a/t/40-st-lims.t +++ b/t/40-st-lims.t @@ -5,7 +5,7 @@ use Test::Exception; use Test::Warn; use File::Temp qw/ tempdir /; -my $num_delegated_methods = 46; +my $num_delegated_methods = 48; local $ENV{'http_proxy'} = 'http://wibble.com'; @@ -433,7 +433,7 @@ subtest 'Object for a tag' => sub { }; subtest 'Object for a non-pool lane' => sub { - plan tests => 97; + plan tests => 99; my $lims = st::api::lims->new(id_run => 6607, position => 1); isa_ok($lims, 'st::api::lims'); diff --git a/t/data/fixtures_lims_wh/000-Sample.yml b/t/data/fixtures_lims_wh/000-Sample.yml index 9fb237d4..8fc7424f 100644 --- a/t/data/fixtures_lims_wh/000-Sample.yml +++ b/t/data/fixtures_lims_wh/000-Sample.yml @@ -462,7 +462,8 @@ cohort: ~ common_name: ~ consent_withdrawn: 0 - control: ~ + control: 1 + control_type: negative country_of_origin: ~ created: 2017-10-19 13:30:14 deleted_at: ~ @@ -492,7 +493,8 @@ cohort: ~ common_name: ~ consent_withdrawn: 0 - control: ~ + control: 1 + control_type: positive country_of_origin: ~ created: 2017-10-19 13:30:14 deleted_at: ~ diff --git a/t/data/samplesheet/1control7libs_extended.csv b/t/data/samplesheet/1control7libs_extended.csv index 47e7e74e..164acddc 100644 --- a/t/data/samplesheet/1control7libs_extended.csv +++ b/t/data/samplesheet/1control7libs_extended.csv @@ -1,10 +1,10 @@ -[Data],,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, -Lane,Sample_ID,Sample_Name,GenomeFolder,bait_name,default_library_type,default_tag_sequence,default_tagtwo_sequence,email_addresses,email_addresses_of_followers,email_addresses_of_managers,email_addresses_of_owners,gbs_plex_name,is_control,is_pool,lane_id,lane_priority,library_name,organism,organism_taxon_id,project_cost_code,project_id,project_name,purpose,qc_state,request_id,required_insert_size_range,sample_accession_number,sample_cohort,sample_common_name,sample_consent_withdrawn,sample_description,sample_donor_id,sample_id,sample_name,sample_public_name,sample_reference_genome,sample_supplier_name,spiked_phix_tag_index,study_accession_number,study_alignments_in_bam,study_contains_nonconsented_human,study_contains_nonconsented_xahuman,study_description,study_id,study_name,study_reference_genome,study_separate_y_chromosome_data,study_title,tag_index, -1,57440,EGAN00001001569,,,,,,dg10@sanger.ac.uk las@sanger.ac.uk pc8@sanger.ac.uk sm2@sanger.ac.uk,dg10@sanger.ac.uk las@sanger.ac.uk pc8@sanger.ac.uk,sm2@sanger.ac.uk,sm2@sanger.ac.uk,,0,0,,,PD3918a 1,Human,9606,S0277,333,CLL whole genome,standard,pass,36189,from:300 to:400,EGAN00001001569,,Homo sapiens,,,,7283,PD3918a,,,,,EGAS00001000014,1,0,0,Genomic libraries (500 bps) will be generated from total genomic DNA derived from a range of cancer samples and subjected paired end sequencing on the llumina GA. Paired reads will be mapped to build 37 of the human reference genome to facilitate the generation of genome wide copy number information%2C and the identification of novel rearranged cancer genes and gene fusions.,333,CLL whole genome,,,CLL Cancer Whole Genome Sequencing,, -2,57440,EGAN00001001569,,,,,,dg10@sanger.ac.uk las@sanger.ac.uk pc8@sanger.ac.uk sm2@sanger.ac.uk,dg10@sanger.ac.uk las@sanger.ac.uk pc8@sanger.ac.uk,sm2@sanger.ac.uk,sm2@sanger.ac.uk,,0,0,,,PD3918a 1,Human,9606,S0277,333,CLL whole genome,standard,pass,36199,from:300 to:400,EGAN00001001569,,Homo sapiens,,,,7283,PD3918a,,,,,EGAS00001000014,1,0,0,Genomic libraries (500 bps) will be generated from total genomic DNA derived from a range of cancer samples and subjected paired end sequencing on the llumina GA. Paired reads will be mapped to build 37 of the human reference genome to facilitate the generation of genome wide copy number information%2C and the identification of novel rearranged cancer genes and gene fusions.,333,CLL whole genome,,,CLL Cancer Whole Genome Sequencing,, -3,57440,EGAN00001001569,,,,,,dg10@sanger.ac.uk las@sanger.ac.uk pc8@sanger.ac.uk sm2@sanger.ac.uk,dg10@sanger.ac.uk las@sanger.ac.uk pc8@sanger.ac.uk,sm2@sanger.ac.uk,sm2@sanger.ac.uk,,0,0,,,PD3918a 1,Human,9606,S0277,333,CLL whole genome,standard,pass,36202,from:300 to:400,EGAN00001001569,,Homo sapiens,,,,7283,PD3918a,,,,,EGAS00001000014,1,0,0,Genomic libraries (500 bps) will be generated from total genomic DNA derived from a range of cancer samples and subjected paired end sequencing on the llumina GA. Paired reads will be mapped to build 37 of the human reference genome to facilitate the generation of genome wide copy number information%2C and the identification of novel rearranged cancer genes and gene fusions.,333,CLL whole genome,,,CLL Cancer Whole Genome Sequencing,, -4,79577,phiX CT1462-2 1,,,,,,,,,,,1,0,,,phiX CT1462-2 1,,,,,,standard,,43779,,,,,,,,9836,phiX CT1462-2 1,,,,,,,0,0,,,,,,,, -5,57440,EGAN00001001569,,,,,,dg10@sanger.ac.uk las@sanger.ac.uk pc8@sanger.ac.uk sm2@sanger.ac.uk,dg10@sanger.ac.uk las@sanger.ac.uk pc8@sanger.ac.uk,sm2@sanger.ac.uk,sm2@sanger.ac.uk,,0,0,,,PD3918a 1,Human,9606,S0277,333,CLL whole genome,standard,pass,36203,from:300 to:400,EGAN00001001569,,Homo sapiens,,,,7283,PD3918a,,,,,EGAS00001000014,1,0,0,Genomic libraries (500 bps) will be generated from total genomic DNA derived from a range of cancer samples and subjected paired end sequencing on the llumina GA. Paired reads will be mapped to build 37 of the human reference genome to facilitate the generation of genome wide copy number information%2C and the identification of novel rearranged cancer genes and gene fusions.,333,CLL whole genome,,,CLL Cancer Whole Genome Sequencing,, -6,57440,EGAN00001001569,,,,,,dg10@sanger.ac.uk las@sanger.ac.uk pc8@sanger.ac.uk sm2@sanger.ac.uk,dg10@sanger.ac.uk las@sanger.ac.uk pc8@sanger.ac.uk,sm2@sanger.ac.uk,sm2@sanger.ac.uk,,0,0,,,PD3918a 1,Human,9606,S0277,333,CLL whole genome,standard,pass,36209,from:300 to:400,EGAN00001001569,,Homo sapiens,,,,7283,PD3918a,,,,,EGAS00001000014,1,0,0,Genomic libraries (500 bps) will be generated from total genomic DNA derived from a range of cancer samples and subjected paired end sequencing on the llumina GA. Paired reads will be mapped to build 37 of the human reference genome to facilitate the generation of genome wide copy number information%2C and the identification of novel rearranged cancer genes and gene fusions.,333,CLL whole genome,,,CLL Cancer Whole Genome Sequencing,, -7,57440,EGAN00001001569,,,,,,dg10@sanger.ac.uk las@sanger.ac.uk pc8@sanger.ac.uk sm2@sanger.ac.uk,dg10@sanger.ac.uk las@sanger.ac.uk pc8@sanger.ac.uk,sm2@sanger.ac.uk,sm2@sanger.ac.uk,,0,0,,,PD3918a 1,Human,9606,S0277,333,CLL whole genome,standard,pass,36210,from:300 to:400,EGAN00001001569,,Homo sapiens,,,,7283,PD3918a,,,,,EGAS00001000014,1,0,0,Genomic libraries (500 bps) will be generated from total genomic DNA derived from a range of cancer samples and subjected paired end sequencing on the llumina GA. Paired reads will be mapped to build 37 of the human reference genome to facilitate the generation of genome wide copy number information%2C and the identification of novel rearranged cancer genes and gene fusions.,333,CLL whole genome,,,CLL Cancer Whole Genome Sequencing,, -8,57440,EGAN00001001569,,,,,,dg10@sanger.ac.uk las@sanger.ac.uk pc8@sanger.ac.uk sm2@sanger.ac.uk,dg10@sanger.ac.uk las@sanger.ac.uk pc8@sanger.ac.uk,sm2@sanger.ac.uk,sm2@sanger.ac.uk,,0,0,,,PD3918a 1,Human,9606,S0277,333,CLL whole genome,standard,pass,36211,from:300 to:400,EGAN00001001569,,Homo sapiens,,,,7283,PD3918a,,,,,EGAS00001000014,1,0,0,Genomic libraries (500 bps) will be generated from total genomic DNA derived from a range of cancer samples and subjected paired end sequencing on the llumina GA. Paired reads will be mapped to build 37 of the human reference genome to facilitate the generation of genome wide copy number information%2C and the identification of novel rearranged cancer genes and gene fusions.,333,CLL whole genome,,,CLL Cancer Whole Genome Sequencing,, +[Data],,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +Lane,Sample_ID,Sample_Name,GenomeFolder,bait_name,default_library_type,default_tag_sequence,default_tagtwo_sequence,email_addresses,email_addresses_of_followers,email_addresses_of_managers,email_addresses_of_owners,gbs_plex_name,is_control,is_pool,lane_id,lane_priority,library_name,organism,organism_taxon_id,project_cost_code,project_id,project_name,purpose,qc_state,request_id,required_insert_size_range,sample_accession_number,sample_cohort,sample_common_name,sample_consent_withdrawn,sample_control_type,sample_description,sample_donor_id,sample_id,sample_is_control,sample_name,sample_public_name,sample_reference_genome,sample_supplier_name,spiked_phix_tag_index,study_accession_number,study_alignments_in_bam,study_contains_nonconsented_human,study_contains_nonconsented_xahuman,study_description,study_id,study_name,study_reference_genome,study_separate_y_chromosome_data,study_title,tag_index, +1,57440,EGAN00001001569,,,,,,dg10@sanger.ac.uk las@sanger.ac.uk pc8@sanger.ac.uk sm2@sanger.ac.uk,dg10@sanger.ac.uk las@sanger.ac.uk pc8@sanger.ac.uk,sm2@sanger.ac.uk,sm2@sanger.ac.uk,,0,0,,,PD3918a 1,Human,9606,S0277,333,CLL whole genome,standard,pass,36189,from:300 to:400,EGAN00001001569,,Homo sapiens,,,,,7283,,PD3918a,,,,,EGAS00001000014,1,0,0,Genomic libraries (500 bps) will be generated from total genomic DNA derived from a range of cancer samples and subjected paired end sequencing on the llumina GA. Paired reads will be mapped to build 37 of the human reference genome to facilitate the generation of genome wide copy number information%2C and the identification of novel rearranged cancer genes and gene fusions.,333,CLL whole genome,,,CLL Cancer Whole Genome Sequencing,, +2,57440,EGAN00001001569,,,,,,dg10@sanger.ac.uk las@sanger.ac.uk pc8@sanger.ac.uk sm2@sanger.ac.uk,dg10@sanger.ac.uk las@sanger.ac.uk pc8@sanger.ac.uk,sm2@sanger.ac.uk,sm2@sanger.ac.uk,,0,0,,,PD3918a 1,Human,9606,S0277,333,CLL whole genome,standard,pass,36199,from:300 to:400,EGAN00001001569,,Homo sapiens,,,,,7283,,PD3918a,,,,,EGAS00001000014,1,0,0,Genomic libraries (500 bps) will be generated from total genomic DNA derived from a range of cancer samples and subjected paired end sequencing on the llumina GA. Paired reads will be mapped to build 37 of the human reference genome to facilitate the generation of genome wide copy number information%2C and the identification of novel rearranged cancer genes and gene fusions.,333,CLL whole genome,,,CLL Cancer Whole Genome Sequencing,, +3,57440,EGAN00001001569,,,,,,dg10@sanger.ac.uk las@sanger.ac.uk pc8@sanger.ac.uk sm2@sanger.ac.uk,dg10@sanger.ac.uk las@sanger.ac.uk pc8@sanger.ac.uk,sm2@sanger.ac.uk,sm2@sanger.ac.uk,,0,0,,,PD3918a 1,Human,9606,S0277,333,CLL whole genome,standard,pass,36202,from:300 to:400,EGAN00001001569,,Homo sapiens,,,,,7283,,PD3918a,,,,,EGAS00001000014,1,0,0,Genomic libraries (500 bps) will be generated from total genomic DNA derived from a range of cancer samples and subjected paired end sequencing on the llumina GA. Paired reads will be mapped to build 37 of the human reference genome to facilitate the generation of genome wide copy number information%2C and the identification of novel rearranged cancer genes and gene fusions.,333,CLL whole genome,,,CLL Cancer Whole Genome Sequencing,, +4,79577,phiX CT1462-2 1,,,,,,,,,,,1,0,,,phiX CT1462-2 1,,,,,,standard,,43779,,,,,,,,,9836,,phiX CT1462-2 1,,,,,,,0,0,,,,,,,, +5,57440,EGAN00001001569,,,,,,dg10@sanger.ac.uk las@sanger.ac.uk pc8@sanger.ac.uk sm2@sanger.ac.uk,dg10@sanger.ac.uk las@sanger.ac.uk pc8@sanger.ac.uk,sm2@sanger.ac.uk,sm2@sanger.ac.uk,,0,0,,,PD3918a 1,Human,9606,S0277,333,CLL whole genome,standard,pass,36203,from:300 to:400,EGAN00001001569,,Homo sapiens,,,,,7283,,PD3918a,,,,,EGAS00001000014,1,0,0,Genomic libraries (500 bps) will be generated from total genomic DNA derived from a range of cancer samples and subjected paired end sequencing on the llumina GA. Paired reads will be mapped to build 37 of the human reference genome to facilitate the generation of genome wide copy number information%2C and the identification of novel rearranged cancer genes and gene fusions.,333,CLL whole genome,,,CLL Cancer Whole Genome Sequencing,, +6,57440,EGAN00001001569,,,,,,dg10@sanger.ac.uk las@sanger.ac.uk pc8@sanger.ac.uk sm2@sanger.ac.uk,dg10@sanger.ac.uk las@sanger.ac.uk pc8@sanger.ac.uk,sm2@sanger.ac.uk,sm2@sanger.ac.uk,,0,0,,,PD3918a 1,Human,9606,S0277,333,CLL whole genome,standard,pass,36209,from:300 to:400,EGAN00001001569,,Homo sapiens,,,,,7283,,PD3918a,,,,,EGAS00001000014,1,0,0,Genomic libraries (500 bps) will be generated from total genomic DNA derived from a range of cancer samples and subjected paired end sequencing on the llumina GA. Paired reads will be mapped to build 37 of the human reference genome to facilitate the generation of genome wide copy number information%2C and the identification of novel rearranged cancer genes and gene fusions.,333,CLL whole genome,,,CLL Cancer Whole Genome Sequencing,, +7,57440,EGAN00001001569,,,,,,dg10@sanger.ac.uk las@sanger.ac.uk pc8@sanger.ac.uk sm2@sanger.ac.uk,dg10@sanger.ac.uk las@sanger.ac.uk pc8@sanger.ac.uk,sm2@sanger.ac.uk,sm2@sanger.ac.uk,,0,0,,,PD3918a 1,Human,9606,S0277,333,CLL whole genome,standard,pass,36210,from:300 to:400,EGAN00001001569,,Homo sapiens,,,,,7283,,PD3918a,,,,,EGAS00001000014,1,0,0,Genomic libraries (500 bps) will be generated from total genomic DNA derived from a range of cancer samples and subjected paired end sequencing on the llumina GA. Paired reads will be mapped to build 37 of the human reference genome to facilitate the generation of genome wide copy number information%2C and the identification of novel rearranged cancer genes and gene fusions.,333,CLL whole genome,,,CLL Cancer Whole Genome Sequencing,, +8,57440,EGAN00001001569,,,,,,dg10@sanger.ac.uk las@sanger.ac.uk pc8@sanger.ac.uk sm2@sanger.ac.uk,dg10@sanger.ac.uk las@sanger.ac.uk pc8@sanger.ac.uk,sm2@sanger.ac.uk,sm2@sanger.ac.uk,,0,0,,,PD3918a 1,Human,9606,S0277,333,CLL whole genome,standard,pass,36211,from:300 to:400,EGAN00001001569,,Homo sapiens,,,,,7283,,PD3918a,,,,,EGAS00001000014,1,0,0,Genomic libraries (500 bps) will be generated from total genomic DNA derived from a range of cancer samples and subjected paired end sequencing on the llumina GA. Paired reads will be mapped to build 37 of the human reference genome to facilitate the generation of genome wide copy number information%2C and the identification of novel rearranged cancer genes and gene fusions.,333,CLL whole genome,,,CLL Cancer Whole Genome Sequencing,, diff --git a/t/data/samplesheet/4pool4libs_extended.csv b/t/data/samplesheet/4pool4libs_extended.csv index d3b3b735..344ab8b1 100644 --- a/t/data/samplesheet/4pool4libs_extended.csv +++ b/t/data/samplesheet/4pool4libs_extended.csv @@ -1,112 +1,112 @@ -[Data],,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, -Lane,Sample_ID,Sample_Name,GenomeFolder,Index,Index2,bait_name,default_library_type,default_tag_sequence,default_tagtwo_sequence,email_addresses,email_addresses_of_followers,email_addresses_of_managers,email_addresses_of_owners,gbs_plex_name,is_control,is_pool,lane_id,lane_priority,library_name,organism,organism_taxon_id,project_cost_code,project_id,project_name,purpose,qc_state,request_id,required_insert_size_range,sample_accession_number,sample_cohort,sample_common_name,sample_consent_withdrawn,sample_description,sample_donor_id,sample_id,sample_name,sample_public_name,sample_reference_genome,sample_supplier_name,spiked_phix_tag_index,study_accession_number,study_alignments_in_bam,study_contains_nonconsented_human,study_contains_nonconsented_xahuman,study_description,study_id,study_name,study_reference_genome,study_separate_y_chromosome_data,study_title,tag_index, -1,7809257,ERS323818,,,,,No PCR,,,hr1@sanger.ac.uk jc17@sanger.ac.uk neh@sanger.ac.uk,jc17@sanger.ac.uk neh@sanger.ac.uk,neh@sanger.ac.uk,hr1@sanger.ac.uk,,0,0,8381746,0,Hc_4_BC4_P2_5046_340285 7809257,Haemonchus contortus,6289,S0702,714,Haemonchus contortus Ivermectin Resistance Genomics Study,standard,,5707613,from:400 to:550,ERS323818,,Haemonchus contortus,,25-30 mixed male and female worms%2C strain identity was checked using a panel of 4 microsatellite loci that discriminate Haemonchus contortus strains,,1660679,Hc_4_BC4_P2_5046_340285,Haemonchus contortus MHco3%2F4.BC4(P2)-5046,Haemonchus_contortus (V1_21June13),,168,ERP000430,,0,0,Two H. contortus ivermectin resistance strains have been backcrossed 4 times against the susceptible genome strain. Parental strains and backcross strains will be sequenced in order to identify regions of the genome linked to ivermectin resistance-conferring loci.,1697,Haemonchus contortus Ivermectin Resistance,,,Haemonchus contortus Ivermectin Resistance,, -2,7809258,ERS323819,,,,,No PCR,,,hr1@sanger.ac.uk jc17@sanger.ac.uk neh@sanger.ac.uk,jc17@sanger.ac.uk neh@sanger.ac.uk,neh@sanger.ac.uk,hr1@sanger.ac.uk,,0,0,8381744,0,Hc_10_BC4_P2_5779_340285 7809258,Haemonchus contortus,6289,S0702,714,Haemonchus contortus Ivermectin Resistance Genomics Study,standard,,5707611,from:400 to:550,ERS323819,,Haemonchus contortus,,25-30 mixed male and female worms%2C strain identity was checked using a panel of 4 microsatellite loci that discriminate Haemonchus contortus strains,,1660680,Hc_10_BC4_P2_5779_340285,Haemonchus contortus MHco3%2F10.BC4(P2)-5779,Haemonchus_contortus (V1_21June13),,168,ERP000430,,0,0,Two H. contortus ivermectin resistance strains have been backcrossed 4 times against the susceptible genome strain. Parental strains and backcross strains will be sequenced in order to identify regions of the genome linked to ivermectin resistance-conferring loci.,1697,Haemonchus contortus Ivermectin Resistance,,,Haemonchus contortus Ivermectin Resistance,, -3,7809258,ERS323819,,,,,No PCR,,,hr1@sanger.ac.uk jc17@sanger.ac.uk neh@sanger.ac.uk,jc17@sanger.ac.uk neh@sanger.ac.uk,neh@sanger.ac.uk,hr1@sanger.ac.uk,,0,0,8381745,0,Hc_10_BC4_P2_5779_340285 7809258,Haemonchus contortus,6289,S0702,714,Haemonchus contortus Ivermectin Resistance Genomics Study,standard,,5707612,from:400 to:550,ERS323819,,Haemonchus contortus,,25-30 mixed male and female worms%2C strain identity was checked using a panel of 4 microsatellite loci that discriminate Haemonchus contortus strains,,1660680,Hc_10_BC4_P2_5779_340285,Haemonchus contortus MHco3%2F10.BC4(P2)-5779,Haemonchus_contortus (V1_21June13),,168,ERP000430,,0,0,Two H. contortus ivermectin resistance strains have been backcrossed 4 times against the susceptible genome strain. Parental strains and backcross strains will be sequenced in order to identify regions of the genome linked to ivermectin resistance-conferring loci.,1697,Haemonchus contortus Ivermectin Resistance,,,Haemonchus contortus Ivermectin Resistance,, -4,8215019,ERS351213,,TAAGGCGA,TAGATCGC,,qPCR only,TAAGGCGATAGATCGC,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell1 8215019,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351213,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706390,mES_ai2_s2_cell1,mES_ai2_s2_cell1,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,1, -4,8215020,ERS351214,,CGTACTAG,TAGATCGC,,qPCR only,CGTACTAGTAGATCGC,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell2 8215020,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351214,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706391,mES_ai2_s2_cell2,mES_ai2_s2_cell2,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,2, -4,8215021,ERS351221,,AGGCAGAA,TAGATCGC,,qPCR only,AGGCAGAATAGATCGC,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell3 8215021,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351221,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706392,mES_ai2_s2_cell3,mES_ai2_s2_cell3,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,3, -4,8215022,ERS351222,,TCCTGAGC,TAGATCGC,,qPCR only,TCCTGAGCTAGATCGC,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell4 8215022,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351222,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706393,mES_ai2_s2_cell4,mES_ai2_s2_cell4,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,4, -4,8215023,ERS351223,,GGACTCCT,TAGATCGC,,qPCR only,GGACTCCTTAGATCGC,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell5 8215023,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351223,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706394,mES_ai2_s2_cell5,mES_ai2_s2_cell5,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,5, -4,8215024,ERS351224,,TAGGCATG,TAGATCGC,,qPCR only,TAGGCATGTAGATCGC,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell6 8215024,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351224,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706395,mES_ai2_s2_cell6,mES_ai2_s2_cell6,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,6, -4,8215025,ERS351225,,CTCTCTAC,TAGATCGC,,qPCR only,CTCTCTACTAGATCGC,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell7 8215025,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351225,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706396,mES_ai2_s2_cell7,mES_ai2_s2_cell7,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,7, -4,8215026,ERS351218,,CAGAGAGG,TAGATCGC,,qPCR only,CAGAGAGGTAGATCGC,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell8 8215026,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351218,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706397,mES_ai2_s2_cell8,mES_ai2_s2_cell8,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,8, -4,8215027,ERS351219,,GCTACGCT,TAGATCGC,,qPCR only,GCTACGCTTAGATCGC,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell9 8215027,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351219,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706398,mES_ai2_s2_cell9,mES_ai2_s2_cell9,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,9, -4,8215028,ERS351220,,CGAGGCTG,TAGATCGC,,qPCR only,CGAGGCTGTAGATCGC,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell10 8215028,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351220,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706399,mES_ai2_s2_cell10,mES_ai2_s2_cell10,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,10, -4,8215029,ERS351235,,AAGAGGCA,TAGATCGC,,qPCR only,AAGAGGCATAGATCGC,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell11 8215029,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351235,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706400,mES_ai2_s2_cell11,mES_ai2_s2_cell11,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,11, -4,8215030,ERS351237,,GTAGAGGA,TAGATCGC,,qPCR only,GTAGAGGATAGATCGC,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell12 8215030,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351237,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706401,mES_ai2_s2_cell12,mES_ai2_s2_cell12,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,12, -4,8215031,ERS351238,,TAAGGCGA,CTCTCTAT,,qPCR only,TAAGGCGACTCTCTAT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell13 8215031,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351238,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706402,mES_ai2_s2_cell13,mES_ai2_s2_cell13,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,13, -4,8215032,ERS351239,,CGTACTAG,CTCTCTAT,,qPCR only,CGTACTAGCTCTCTAT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell14 8215032,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351239,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706403,mES_ai2_s2_cell14,mES_ai2_s2_cell14,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,14, -4,8215033,ERS351241,,AGGCAGAA,CTCTCTAT,,qPCR only,AGGCAGAACTCTCTAT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell15 8215033,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351241,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706404,mES_ai2_s2_cell15,mES_ai2_s2_cell15,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,15, -4,8215034,ERS351226,,TCCTGAGC,CTCTCTAT,,qPCR only,TCCTGAGCCTCTCTAT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell16 8215034,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351226,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706405,mES_ai2_s2_cell16,mES_ai2_s2_cell16,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,16, -4,8215035,ERS351227,,GGACTCCT,CTCTCTAT,,qPCR only,GGACTCCTCTCTCTAT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell17 8215035,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351227,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706406,mES_ai2_s2_cell17,mES_ai2_s2_cell17,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,17, -4,8215036,ERS351234,,TAGGCATG,CTCTCTAT,,qPCR only,TAGGCATGCTCTCTAT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell18 8215036,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351234,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706407,mES_ai2_s2_cell18,mES_ai2_s2_cell18,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,18, -4,8215037,ERS351236,,CTCTCTAC,CTCTCTAT,,qPCR only,CTCTCTACCTCTCTAT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell19 8215037,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351236,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706408,mES_ai2_s2_cell19,mES_ai2_s2_cell19,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,19, -4,8215038,ERS351247,,CAGAGAGG,CTCTCTAT,,qPCR only,CAGAGAGGCTCTCTAT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell20 8215038,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351247,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706409,mES_ai2_s2_cell20,mES_ai2_s2_cell20,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,20, -4,8215039,ERS351249,,GCTACGCT,CTCTCTAT,,qPCR only,GCTACGCTCTCTCTAT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell21 8215039,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351249,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706410,mES_ai2_s2_cell21,mES_ai2_s2_cell21,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,21, -4,8215040,ERS351251,,CGAGGCTG,CTCTCTAT,,qPCR only,CGAGGCTGCTCTCTAT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell22 8215040,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351251,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706411,mES_ai2_s2_cell22,mES_ai2_s2_cell22,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,22, -4,8215041,ERS351252,,AAGAGGCA,CTCTCTAT,,qPCR only,AAGAGGCACTCTCTAT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell23 8215041,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351252,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706412,mES_ai2_s2_cell23,mES_ai2_s2_cell23,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,23, -4,8215042,ERS351242,,GTAGAGGA,CTCTCTAT,,qPCR only,GTAGAGGACTCTCTAT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell24 8215042,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351242,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706413,mES_ai2_s2_cell24,mES_ai2_s2_cell24,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,24, -4,8215043,ERS351243,,TAAGGCGA,TATCCTCT,,qPCR only,TAAGGCGATATCCTCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell25 8215043,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351243,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706414,mES_ai2_s2_cell25,mES_ai2_s2_cell25,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,25, -4,8215044,ERS351244,,CGTACTAG,TATCCTCT,,qPCR only,CGTACTAGTATCCTCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell26 8215044,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351244,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706415,mES_ai2_s2_cell26,mES_ai2_s2_cell26,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,26, -4,8215045,ERS351245,,AGGCAGAA,TATCCTCT,,qPCR only,AGGCAGAATATCCTCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell27 8215045,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351245,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706416,mES_ai2_s2_cell27,mES_ai2_s2_cell27,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,27, -4,8215046,ERS351248,,TCCTGAGC,TATCCTCT,,qPCR only,TCCTGAGCTATCCTCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell28 8215046,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351248,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706417,mES_ai2_s2_cell28,mES_ai2_s2_cell28,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,28, -4,8215047,ERS351250,,GGACTCCT,TATCCTCT,,qPCR only,GGACTCCTTATCCTCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell29 8215047,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351250,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706418,mES_ai2_s2_cell29,mES_ai2_s2_cell29,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,29, -4,8215048,ERS351260,,TAGGCATG,TATCCTCT,,qPCR only,TAGGCATGTATCCTCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell30 8215048,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351260,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706419,mES_ai2_s2_cell30,mES_ai2_s2_cell30,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,30, -4,8215049,ERS351261,,CTCTCTAC,TATCCTCT,,qPCR only,CTCTCTACTATCCTCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell31 8215049,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351261,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706420,mES_ai2_s2_cell31,mES_ai2_s2_cell31,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,31, -4,8215050,ERS351254,,CAGAGAGG,TATCCTCT,,qPCR only,CAGAGAGGTATCCTCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell32 8215050,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351254,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706421,mES_ai2_s2_cell32,mES_ai2_s2_cell32,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,32, -4,8215051,ERS351255,,GCTACGCT,TATCCTCT,,qPCR only,GCTACGCTTATCCTCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell33 8215051,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351255,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706422,mES_ai2_s2_cell33,mES_ai2_s2_cell33,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,33, -4,8215052,ERS351256,,CGAGGCTG,TATCCTCT,,qPCR only,CGAGGCTGTATCCTCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell34 8215052,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351256,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706423,mES_ai2_s2_cell34,mES_ai2_s2_cell34,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,34, -4,8215053,ERS351257,,AAGAGGCA,TATCCTCT,,qPCR only,AAGAGGCATATCCTCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell35 8215053,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351257,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706424,mES_ai2_s2_cell35,mES_ai2_s2_cell35,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,35, -4,8215054,ERS351258,,GTAGAGGA,TATCCTCT,,qPCR only,GTAGAGGATATCCTCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell36 8215054,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351258,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706425,mES_ai2_s2_cell36,mES_ai2_s2_cell36,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,36, -4,8215055,ERS351259,,TAAGGCGA,AGAGTAGA,,qPCR only,TAAGGCGAAGAGTAGA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell37 8215055,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351259,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706426,mES_ai2_s2_cell37,mES_ai2_s2_cell37,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,37, -4,8215056,ERS351269,,CGTACTAG,AGAGTAGA,,qPCR only,CGTACTAGAGAGTAGA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell38 8215056,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351269,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706427,mES_ai2_s2_cell38,mES_ai2_s2_cell38,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,38, -4,8215057,ERS351271,,AGGCAGAA,AGAGTAGA,,qPCR only,AGGCAGAAAGAGTAGA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell39 8215057,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351271,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706428,mES_ai2_s2_cell39,mES_ai2_s2_cell39,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,39, -4,8215058,ERS351262,,TCCTGAGC,AGAGTAGA,,qPCR only,TCCTGAGCAGAGTAGA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell40 8215058,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351262,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706429,mES_ai2_s2_cell40,mES_ai2_s2_cell40,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,40, -4,8215059,ERS351263,,GGACTCCT,AGAGTAGA,,qPCR only,GGACTCCTAGAGTAGA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell41 8215059,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351263,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706430,mES_ai2_s2_cell41,mES_ai2_s2_cell41,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,41, -4,8215060,ERS351264,,TAGGCATG,AGAGTAGA,,qPCR only,TAGGCATGAGAGTAGA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell42 8215060,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351264,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706431,mES_ai2_s2_cell42,mES_ai2_s2_cell42,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,42, -4,8215061,ERS351266,,CTCTCTAC,AGAGTAGA,,qPCR only,CTCTCTACAGAGTAGA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell43 8215061,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351266,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706432,mES_ai2_s2_cell43,mES_ai2_s2_cell43,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,43, -4,8215062,ERS351267,,CAGAGAGG,AGAGTAGA,,qPCR only,CAGAGAGGAGAGTAGA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell44 8215062,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351267,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706433,mES_ai2_s2_cell44,mES_ai2_s2_cell44,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,44, -4,8215063,ERS351268,,GCTACGCT,AGAGTAGA,,qPCR only,GCTACGCTAGAGTAGA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell45 8215063,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351268,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706434,mES_ai2_s2_cell45,mES_ai2_s2_cell45,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,45, -4,8215064,ERS351270,,CGAGGCTG,AGAGTAGA,,qPCR only,CGAGGCTGAGAGTAGA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell46 8215064,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351270,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706435,mES_ai2_s2_cell46,mES_ai2_s2_cell46,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,46, -4,8215065,ERS351272,,AAGAGGCA,AGAGTAGA,,qPCR only,AAGAGGCAAGAGTAGA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell47 8215065,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351272,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706436,mES_ai2_s2_cell47,mES_ai2_s2_cell47,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,47, -4,8215066,ERS351273,,GTAGAGGA,AGAGTAGA,,qPCR only,GTAGAGGAAGAGTAGA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell48 8215066,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351273,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706437,mES_ai2_s2_cell48,mES_ai2_s2_cell48,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,48, -4,8215067,ERS351275,,TAAGGCGA,GTAAGGAG,,qPCR only,TAAGGCGAGTAAGGAG,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell49 8215067,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351275,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706438,mES_ai2_s2_cell49,mES_ai2_s2_cell49,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,49, -4,8215068,ERS351277,,CGTACTAG,GTAAGGAG,,qPCR only,CGTACTAGGTAAGGAG,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell50 8215068,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351277,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706439,mES_ai2_s2_cell50,mES_ai2_s2_cell50,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,50, -4,8215069,ERS351278,,AGGCAGAA,GTAAGGAG,,qPCR only,AGGCAGAAGTAAGGAG,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell51 8215069,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351278,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706440,mES_ai2_s2_cell51,mES_ai2_s2_cell51,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,51, -4,8215070,ERS351279,,TCCTGAGC,GTAAGGAG,,qPCR only,TCCTGAGCGTAAGGAG,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell52 8215070,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351279,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706441,mES_ai2_s2_cell52,mES_ai2_s2_cell52,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,52, -4,8215071,ERS351280,,GGACTCCT,GTAAGGAG,,qPCR only,GGACTCCTGTAAGGAG,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell53 8215071,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351280,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706442,mES_ai2_s2_cell53,mES_ai2_s2_cell53,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,53, -4,8215072,ERS351281,,TAGGCATG,GTAAGGAG,,qPCR only,TAGGCATGGTAAGGAG,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell54 8215072,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351281,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706443,mES_ai2_s2_cell54,mES_ai2_s2_cell54,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,54, -4,8215073,ERS351282,,CTCTCTAC,GTAAGGAG,,qPCR only,CTCTCTACGTAAGGAG,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell55 8215073,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351282,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706444,mES_ai2_s2_cell55,mES_ai2_s2_cell55,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,55, -4,8215074,ERS351274,,CAGAGAGG,GTAAGGAG,,qPCR only,CAGAGAGGGTAAGGAG,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell56 8215074,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351274,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706445,mES_ai2_s2_cell56,mES_ai2_s2_cell56,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,56, -4,8215075,ERS351276,,GCTACGCT,GTAAGGAG,,qPCR only,GCTACGCTGTAAGGAG,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell57 8215075,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351276,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706446,mES_ai2_s2_cell57,mES_ai2_s2_cell57,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,57, -4,8215076,ERS351285,,CGAGGCTG,GTAAGGAG,,qPCR only,CGAGGCTGGTAAGGAG,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell58 8215076,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351285,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706447,mES_ai2_s2_cell58,mES_ai2_s2_cell58,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,58, -4,8215077,ERS351286,,AAGAGGCA,GTAAGGAG,,qPCR only,AAGAGGCAGTAAGGAG,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell59 8215077,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351286,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706448,mES_ai2_s2_cell59,mES_ai2_s2_cell59,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,59, -4,8215078,ERS351287,,GTAGAGGA,GTAAGGAG,,qPCR only,GTAGAGGAGTAAGGAG,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell60 8215078,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351287,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706449,mES_ai2_s2_cell60,mES_ai2_s2_cell60,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,60, -4,8215079,ERS351288,,TAAGGCGA,ACTGCATA,,qPCR only,TAAGGCGAACTGCATA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell61 8215079,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351288,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706450,mES_ai2_s2_cell61,mES_ai2_s2_cell61,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,61, -4,8215080,ERS351289,,CGTACTAG,ACTGCATA,,qPCR only,CGTACTAGACTGCATA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell62 8215080,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351289,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706451,mES_ai2_s2_cell62,mES_ai2_s2_cell62,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,62, -4,8215081,ERS351290,,AGGCAGAA,ACTGCATA,,qPCR only,AGGCAGAAACTGCATA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell63 8215081,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351290,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706452,mES_ai2_s2_cell63,mES_ai2_s2_cell63,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,63, -4,8215082,ERS351283,,TCCTGAGC,ACTGCATA,,qPCR only,TCCTGAGCACTGCATA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell64 8215082,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351283,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706453,mES_ai2_s2_cell64,mES_ai2_s2_cell64,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,64, -4,8215083,ERS351284,,GGACTCCT,ACTGCATA,,qPCR only,GGACTCCTACTGCATA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell65 8215083,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351284,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706454,mES_ai2_s2_cell65,mES_ai2_s2_cell65,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,65, -4,8215084,ERS351292,,TAGGCATG,ACTGCATA,,qPCR only,TAGGCATGACTGCATA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell66 8215084,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351292,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706455,mES_ai2_s2_cell66,mES_ai2_s2_cell66,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,66, -4,8215085,ERS351293,,CTCTCTAC,ACTGCATA,,qPCR only,CTCTCTACACTGCATA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell67 8215085,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351293,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706456,mES_ai2_s2_cell67,mES_ai2_s2_cell67,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,67, -4,8215086,ERS351294,,CAGAGAGG,ACTGCATA,,qPCR only,CAGAGAGGACTGCATA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell68 8215086,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351294,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706457,mES_ai2_s2_cell68,mES_ai2_s2_cell68,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,68, -4,8215087,ERS351295,,GCTACGCT,ACTGCATA,,qPCR only,GCTACGCTACTGCATA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell69 8215087,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351295,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706458,mES_ai2_s2_cell69,mES_ai2_s2_cell69,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,69, -4,8215088,ERS351296,,CGAGGCTG,ACTGCATA,,qPCR only,CGAGGCTGACTGCATA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell70 8215088,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351296,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706459,mES_ai2_s2_cell70,mES_ai2_s2_cell70,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,70, -4,8215089,ERS351297,,AAGAGGCA,ACTGCATA,,qPCR only,AAGAGGCAACTGCATA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell71 8215089,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351297,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706460,mES_ai2_s2_cell71,mES_ai2_s2_cell71,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,71, -4,8215090,ERS351291,,GTAGAGGA,ACTGCATA,,qPCR only,GTAGAGGAACTGCATA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell72 8215090,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351291,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706461,mES_ai2_s2_cell72,mES_ai2_s2_cell72,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,72, -4,8215091,ERS351299,,TAAGGCGA,AAGGAGTA,,qPCR only,TAAGGCGAAAGGAGTA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell73 8215091,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351299,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706462,mES_ai2_s2_cell73,mES_ai2_s2_cell73,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,73, -4,8215092,ERS351301,,CGTACTAG,AAGGAGTA,,qPCR only,CGTACTAGAAGGAGTA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell74 8215092,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351301,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706463,mES_ai2_s2_cell74,mES_ai2_s2_cell74,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,74, -4,8215093,ERS351302,,AGGCAGAA,AAGGAGTA,,qPCR only,AGGCAGAAAAGGAGTA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell75 8215093,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351302,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706464,mES_ai2_s2_cell75,mES_ai2_s2_cell75,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,75, -4,8215094,ERS351303,,TCCTGAGC,AAGGAGTA,,qPCR only,TCCTGAGCAAGGAGTA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell76 8215094,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351303,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706465,mES_ai2_s2_cell76,mES_ai2_s2_cell76,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,76, -4,8215095,ERS351304,,GGACTCCT,AAGGAGTA,,qPCR only,GGACTCCTAAGGAGTA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell77 8215095,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351304,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706466,mES_ai2_s2_cell77,mES_ai2_s2_cell77,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,77, -4,8215096,ERS351305,,TAGGCATG,AAGGAGTA,,qPCR only,TAGGCATGAAGGAGTA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell78 8215096,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351305,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706467,mES_ai2_s2_cell78,mES_ai2_s2_cell78,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,78, -4,8215097,ERS351306,,CTCTCTAC,AAGGAGTA,,qPCR only,CTCTCTACAAGGAGTA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell79 8215097,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351306,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706468,mES_ai2_s2_cell79,mES_ai2_s2_cell79,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,79, -4,8215098,ERS351298,,CAGAGAGG,AAGGAGTA,,qPCR only,CAGAGAGGAAGGAGTA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell80 8215098,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351298,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706469,mES_ai2_s2_cell80,mES_ai2_s2_cell80,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,80, -4,8215099,ERS351300,,GCTACGCT,AAGGAGTA,,qPCR only,GCTACGCTAAGGAGTA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell81 8215099,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351300,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706470,mES_ai2_s2_cell81,mES_ai2_s2_cell81,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,81, -4,8215100,ERS351309,,CGAGGCTG,AAGGAGTA,,qPCR only,CGAGGCTGAAGGAGTA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell82 8215100,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351309,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706471,mES_ai2_s2_cell82,mES_ai2_s2_cell82,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,82, -4,8215101,ERS351310,,AAGAGGCA,AAGGAGTA,,qPCR only,AAGAGGCAAAGGAGTA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell83 8215101,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351310,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706472,mES_ai2_s2_cell83,mES_ai2_s2_cell83,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,83, -4,8215102,ERS351311,,GTAGAGGA,AAGGAGTA,,qPCR only,GTAGAGGAAAGGAGTA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell84 8215102,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351311,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706473,mES_ai2_s2_cell84,mES_ai2_s2_cell84,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,84, -4,8215103,ERS351312,,TAAGGCGA,CTAAGCCT,,qPCR only,TAAGGCGACTAAGCCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell85 8215103,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351312,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706474,mES_ai2_s2_cell85,mES_ai2_s2_cell85,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,85, -4,8215104,ERS351313,,CGTACTAG,CTAAGCCT,,qPCR only,CGTACTAGCTAAGCCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell86 8215104,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351313,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706475,mES_ai2_s2_cell86,mES_ai2_s2_cell86,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,86, -4,8215105,ERS351314,,AGGCAGAA,CTAAGCCT,,qPCR only,AGGCAGAACTAAGCCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell87 8215105,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351314,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706476,mES_ai2_s2_cell87,mES_ai2_s2_cell87,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,87, -4,8215106,ERS351307,,TCCTGAGC,CTAAGCCT,,qPCR only,TCCTGAGCCTAAGCCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell88 8215106,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351307,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706477,mES_ai2_s2_cell88,mES_ai2_s2_cell88,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,88, -4,8215107,ERS351308,,GGACTCCT,CTAAGCCT,,qPCR only,GGACTCCTCTAAGCCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell89 8215107,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351308,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706478,mES_ai2_s2_cell89,mES_ai2_s2_cell89,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,89, -4,8215108,ERS351315,,TAGGCATG,CTAAGCCT,,qPCR only,TAGGCATGCTAAGCCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell90 8215108,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351315,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706479,mES_ai2_s2_cell90,mES_ai2_s2_cell90,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,90, -4,8215109,ERS351316,,CTCTCTAC,CTAAGCCT,,qPCR only,CTCTCTACCTAAGCCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell91 8215109,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351316,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706480,mES_ai2_s2_cell91,mES_ai2_s2_cell91,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,91, -4,8215110,ERS351317,,CAGAGAGG,CTAAGCCT,,qPCR only,CAGAGAGGCTAAGCCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell92 8215110,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351317,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706481,mES_ai2_s2_cell92,mES_ai2_s2_cell92,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,92, -4,8215111,ERS351318,,GCTACGCT,CTAAGCCT,,qPCR only,GCTACGCTCTAAGCCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell93 8215111,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351318,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706482,mES_ai2_s2_cell93,mES_ai2_s2_cell93,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,93, -4,8215112,ERS351319,,CGAGGCTG,CTAAGCCT,,qPCR only,CGAGGCTGCTAAGCCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell94 8215112,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351319,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706483,mES_ai2_s2_cell94,mES_ai2_s2_cell94,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,94, -4,8215113,ERS351320,,AAGAGGCA,CTAAGCCT,,qPCR only,AAGAGGCACTAAGCCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell95 8215113,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351320,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706484,mES_ai2_s2_cell95,mES_ai2_s2_cell95,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,95, -4,8215114,ERS351321,,GTAGAGGA,CTAAGCCT,,qPCR only,GTAGAGGACTAAGCCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell96 8215114,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351321,,Mus Musculus,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706485,mES_ai2_s2_cell96,mES_ai2_s2_cell96,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,96, -4,6200285,phiX_for_spiked_buffers,,ACAACGCAAT,,,,ACAACGCAAT,,hps@sanger.ac.uk,,hps@sanger.ac.uk,hps@sanger.ac.uk,,1,0,,,PhiX_PS_20120919,,,,,,standard,,,,,,,,,,1255141,phiX_for_spiked_buffers,,,,168,,1,0,0,None,198,Illumina Controls,,,,168, -5,8269740,EGAN00001173643,,,,,No PCR,,,cdt@sanger.ac.uk cs4@sanger.ac.uk je6@sanger.ac.uk las@sanger.ac.uk lm5@sanger.ac.uk sm2@sanger.ac.uk som@sanger.ac.uk,cdt@sanger.ac.uk je6@sanger.ac.uk las@sanger.ac.uk som@sanger.ac.uk,cdt@sanger.ac.uk cs4@sanger.ac.uk lm5@sanger.ac.uk sm2@sanger.ac.uk,sm2@sanger.ac.uk,,0,0,8381740,0,PD14393b_wg 8269740,Human,9606,S0814,1422,CGP Core Sequencing 10%2F13 to 09%2F14,standard,,5676016,from:300 to:500,EGAN00001173643,,Homo sapiens,,,,1712041,PD14393b_wg,PD14393b,,,168,EGAS00001000290,1,0,0,Wholegenome libraries will be prepared from at least two serial samples reflecting different stages of disease progression and matched constitutional DNA for 30 Myeloproliferative Disease samples. Five lanes of Illumina HiSeq sequencing will be performed on each of the tumour samples and four lanes for each of the constitutional DNA. Sequencing data will mapped to build 37 of the human reference genome and analysis will be performed to characterize the spectrum of somatic variation present in these samples including single base pair mutations%2C insertions%2C deletions as well as larger structural variants and genomic rearrangements. ,2239,MPN Whole Genomes,Homo_sapiens (CGP_GRCh37.NCBI.allchr_MT),,Myeloproliferative Disease Whole Genomes,, -6,8324592,ERS354532,,GAGATTCC,TAATCTTA,,Pre-quality controlled,GAGATTCCTAATCTTA,,ms27@sanger.ac.uk ncb@sanger.ac.uk,,ncb@sanger.ac.uk,ms27@sanger.ac.uk,,0,0,,,T_BCM1_F4 8324592,Mouse,10090,S1553,1238,Mouse model to quantify genotype-epigenotype variations,standard,,,from:100 to:1000,ERS354532,,Mus musculus,,RNA,,1694494,T_BCM1_F4,RT_37,,,168,ERP002223,1,0,0,In order to examine the impact of genetic variation on epigenetic marking and control of gene expression%2C this study has been initiated using two inbred strains of mice%2C C57BL%2F6J and CAST%2FEij which genomes indicate substantial structural and nucleotide variation. Stocks of adult C57BL%2F6J and CAST%2FEij mice as well as CAST%2FBL6 and BL6%2FCAST reciprocal hybrids are employed for isolation of pure and synchronous populations of B and T-lymphocytes. RNA-Seq expression analysis will be performed in total RNA extracted from B-lymphocytes isolated from males and females of both strains and hybrids.%0A %0AThis data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria)%2C please see http%3A%2F%2Fwww.sanger.ac.uk%2Fdatasharing%2F,2501,Mouse model to quantify genotype-epigenotype variations_RNA,Mus_musculus (GRCm38),,Mouse model to quantify genotype-epigenotype variations ,64, -6,8324593,ERS354533,,ATTCAGAA,TAATCTTA,,Pre-quality controlled,ATTCAGAATAATCTTA,,ms27@sanger.ac.uk ncb@sanger.ac.uk,,ncb@sanger.ac.uk,ms27@sanger.ac.uk,,0,0,,,T_BCM1_F5 8324593,Mouse,10090,S1553,1238,Mouse model to quantify genotype-epigenotype variations,standard,,,from:100 to:1000,ERS354533,,Mus musculus,,RNA,,1694495,T_BCM1_F5,RT_38,,,168,ERP002223,1,0,0,In order to examine the impact of genetic variation on epigenetic marking and control of gene expression%2C this study has been initiated using two inbred strains of mice%2C C57BL%2F6J and CAST%2FEij which genomes indicate substantial structural and nucleotide variation. Stocks of adult C57BL%2F6J and CAST%2FEij mice as well as CAST%2FBL6 and BL6%2FCAST reciprocal hybrids are employed for isolation of pure and synchronous populations of B and T-lymphocytes. RNA-Seq expression analysis will be performed in total RNA extracted from B-lymphocytes isolated from males and females of both strains and hybrids.%0A %0AThis data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria)%2C please see http%3A%2F%2Fwww.sanger.ac.uk%2Fdatasharing%2F,2501,Mouse model to quantify genotype-epigenotype variations_RNA,Mus_musculus (GRCm38),,Mouse model to quantify genotype-epigenotype variations ,65, -6,6200285,phiX_for_spiked_buffers,,ACAACGCAAT,,,,ACAACGCAAT,,hps@sanger.ac.uk,,hps@sanger.ac.uk,hps@sanger.ac.uk,,1,0,,,PhiX_PS_20120919,,,,,,standard,,,,,,,,,,1255141,phiX_for_spiked_buffers,,,,168,,1,0,0,None,198,Illumina Controls,,,,168, -7,8324594,ERS354534,,GAGATTCC,CAGGACGT,,Pre-quality controlled,GAGATTCCCAGGACGT,,ms27@sanger.ac.uk ncb@sanger.ac.uk,,ncb@sanger.ac.uk,ms27@sanger.ac.uk,,0,0,,,T_CBF1_G4 8324594,Mouse,10090,S1553,1238,Mouse model to quantify genotype-epigenotype variations,standard,,,from:100 to:1000,ERS354534,,Mus musculus,,RNA,,1694496,T_CBF1_G4,RT_39,,,168,ERP002223,1,0,0,In order to examine the impact of genetic variation on epigenetic marking and control of gene expression%2C this study has been initiated using two inbred strains of mice%2C C57BL%2F6J and CAST%2FEij which genomes indicate substantial structural and nucleotide variation. Stocks of adult C57BL%2F6J and CAST%2FEij mice as well as CAST%2FBL6 and BL6%2FCAST reciprocal hybrids are employed for isolation of pure and synchronous populations of B and T-lymphocytes. RNA-Seq expression analysis will be performed in total RNA extracted from B-lymphocytes isolated from males and females of both strains and hybrids.%0A %0AThis data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria)%2C please see http%3A%2F%2Fwww.sanger.ac.uk%2Fdatasharing%2F,2501,Mouse model to quantify genotype-epigenotype variations_RNA,Mus_musculus (GRCm38),,Mouse model to quantify genotype-epigenotype variations ,76, -7,8324595,ERS354535,,ATTCAGAA,CAGGACGT,,Pre-quality controlled,ATTCAGAACAGGACGT,,ms27@sanger.ac.uk ncb@sanger.ac.uk,,ncb@sanger.ac.uk,ms27@sanger.ac.uk,,0,0,,,T_CBF1_G5 8324595,Mouse,10090,S1553,1238,Mouse model to quantify genotype-epigenotype variations,standard,,,from:100 to:1000,ERS354535,,Mus musculus,,RNA,,1694497,T_CBF1_G5,RT_40,,,168,ERP002223,1,0,0,In order to examine the impact of genetic variation on epigenetic marking and control of gene expression%2C this study has been initiated using two inbred strains of mice%2C C57BL%2F6J and CAST%2FEij which genomes indicate substantial structural and nucleotide variation. Stocks of adult C57BL%2F6J and CAST%2FEij mice as well as CAST%2FBL6 and BL6%2FCAST reciprocal hybrids are employed for isolation of pure and synchronous populations of B and T-lymphocytes. RNA-Seq expression analysis will be performed in total RNA extracted from B-lymphocytes isolated from males and females of both strains and hybrids.%0A %0AThis data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria)%2C please see http%3A%2F%2Fwww.sanger.ac.uk%2Fdatasharing%2F,2501,Mouse model to quantify genotype-epigenotype variations_RNA,Mus_musculus (GRCm38),,Mouse model to quantify genotype-epigenotype variations ,77, -7,6200285,phiX_for_spiked_buffers,,ACAACGCAAT,,,,ACAACGCAAT,,hps@sanger.ac.uk,,hps@sanger.ac.uk,hps@sanger.ac.uk,,1,0,,,PhiX_PS_20120919,,,,,,standard,,,,,,,,,,1255141,phiX_for_spiked_buffers,,,,168,,1,0,0,None,198,Illumina Controls,,,,168, -8,8324596,ERS354536,,GAGATTCC,GTACTGAC,,Pre-quality controlled,GAGATTCCGTACTGAC,,ms27@sanger.ac.uk ncb@sanger.ac.uk,,ncb@sanger.ac.uk,ms27@sanger.ac.uk,,0,0,,,T_CBM2_H4 8324596,Mouse,10090,S1553,1238,Mouse model to quantify genotype-epigenotype variations,standard,,,from:100 to:1000,ERS354536,,Mus musculus,,RNA,,1694498,T_CBM2_H4,RT_41,,,168,ERP002223,1,0,0,In order to examine the impact of genetic variation on epigenetic marking and control of gene expression%2C this study has been initiated using two inbred strains of mice%2C C57BL%2F6J and CAST%2FEij which genomes indicate substantial structural and nucleotide variation. Stocks of adult C57BL%2F6J and CAST%2FEij mice as well as CAST%2FBL6 and BL6%2FCAST reciprocal hybrids are employed for isolation of pure and synchronous populations of B and T-lymphocytes. RNA-Seq expression analysis will be performed in total RNA extracted from B-lymphocytes isolated from males and females of both strains and hybrids.%0A %0AThis data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria)%2C please see http%3A%2F%2Fwww.sanger.ac.uk%2Fdatasharing%2F,2501,Mouse model to quantify genotype-epigenotype variations_RNA,Mus_musculus (GRCm38),,Mouse model to quantify genotype-epigenotype variations ,88, -8,8324597,ERS354537,,ATTCAGAA,GTACTGAC,,Pre-quality controlled,ATTCAGAAGTACTGAC,,ms27@sanger.ac.uk ncb@sanger.ac.uk,,ncb@sanger.ac.uk,ms27@sanger.ac.uk,,0,0,,,T_CBM2_H5 8324597,Mouse,10090,S1553,1238,Mouse model to quantify genotype-epigenotype variations,standard,,,from:100 to:1000,ERS354537,,Mus musculus,,RNA,,1694499,T_CBM2_H5,RT_42,,,168,ERP002223,1,0,0,In order to examine the impact of genetic variation on epigenetic marking and control of gene expression%2C this study has been initiated using two inbred strains of mice%2C C57BL%2F6J and CAST%2FEij which genomes indicate substantial structural and nucleotide variation. Stocks of adult C57BL%2F6J and CAST%2FEij mice as well as CAST%2FBL6 and BL6%2FCAST reciprocal hybrids are employed for isolation of pure and synchronous populations of B and T-lymphocytes. RNA-Seq expression analysis will be performed in total RNA extracted from B-lymphocytes isolated from males and females of both strains and hybrids.%0A %0AThis data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria)%2C please see http%3A%2F%2Fwww.sanger.ac.uk%2Fdatasharing%2F,2501,Mouse model to quantify genotype-epigenotype variations_RNA,Mus_musculus (GRCm38),,Mouse model to quantify genotype-epigenotype variations ,89, -8,6200285,phiX_for_spiked_buffers,,ACAACGCAAT,,,,ACAACGCAAT,,hps@sanger.ac.uk,,hps@sanger.ac.uk,hps@sanger.ac.uk,,1,0,,,PhiX_PS_20120919,,,,,,standard,,,,,,,,,,1255141,phiX_for_spiked_buffers,,,,168,,1,0,0,None,198,Illumina Controls,,,,168, +[Data],,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +Lane,Sample_ID,Sample_Name,GenomeFolder,Index,Index2,bait_name,default_library_type,default_tag_sequence,default_tagtwo_sequence,email_addresses,email_addresses_of_followers,email_addresses_of_managers,email_addresses_of_owners,gbs_plex_name,is_control,is_pool,lane_id,lane_priority,library_name,organism,organism_taxon_id,project_cost_code,project_id,project_name,purpose,qc_state,request_id,required_insert_size_range,sample_accession_number,sample_cohort,sample_common_name,sample_consent_withdrawn,sample_control_type,sample_description,sample_donor_id,sample_id,sample_is_control,sample_name,sample_public_name,sample_reference_genome,sample_supplier_name,spiked_phix_tag_index,study_accession_number,study_alignments_in_bam,study_contains_nonconsented_human,study_contains_nonconsented_xahuman,study_description,study_id,study_name,study_reference_genome,study_separate_y_chromosome_data,study_title,tag_index, +1,7809257,ERS323818,,,,,No PCR,,,hr1@sanger.ac.uk jc17@sanger.ac.uk neh@sanger.ac.uk,jc17@sanger.ac.uk neh@sanger.ac.uk,neh@sanger.ac.uk,hr1@sanger.ac.uk,,0,0,8381746,0,Hc_4_BC4_P2_5046_340285 7809257,Haemonchus contortus,6289,S0702,714,Haemonchus contortus Ivermectin Resistance Genomics Study,standard,,5707613,from:400 to:550,ERS323818,,Haemonchus contortus,,,25-30 mixed male and female worms%2C strain identity was checked using a panel of 4 microsatellite loci that discriminate Haemonchus contortus strains,,1660679,,Hc_4_BC4_P2_5046_340285,Haemonchus contortus MHco3%2F4.BC4(P2)-5046,Haemonchus_contortus (V1_21June13),,168,ERP000430,,0,0,Two H. contortus ivermectin resistance strains have been backcrossed 4 times against the susceptible genome strain. Parental strains and backcross strains will be sequenced in order to identify regions of the genome linked to ivermectin resistance-conferring loci.,1697,Haemonchus contortus Ivermectin Resistance,,,Haemonchus contortus Ivermectin Resistance,, +2,7809258,ERS323819,,,,,No PCR,,,hr1@sanger.ac.uk jc17@sanger.ac.uk neh@sanger.ac.uk,jc17@sanger.ac.uk neh@sanger.ac.uk,neh@sanger.ac.uk,hr1@sanger.ac.uk,,0,0,8381744,0,Hc_10_BC4_P2_5779_340285 7809258,Haemonchus contortus,6289,S0702,714,Haemonchus contortus Ivermectin Resistance Genomics Study,standard,,5707611,from:400 to:550,ERS323819,,Haemonchus contortus,,,25-30 mixed male and female worms%2C strain identity was checked using a panel of 4 microsatellite loci that discriminate Haemonchus contortus strains,,1660680,,Hc_10_BC4_P2_5779_340285,Haemonchus contortus MHco3%2F10.BC4(P2)-5779,Haemonchus_contortus (V1_21June13),,168,ERP000430,,0,0,Two H. contortus ivermectin resistance strains have been backcrossed 4 times against the susceptible genome strain. Parental strains and backcross strains will be sequenced in order to identify regions of the genome linked to ivermectin resistance-conferring loci.,1697,Haemonchus contortus Ivermectin Resistance,,,Haemonchus contortus Ivermectin Resistance,, +3,7809258,ERS323819,,,,,No PCR,,,hr1@sanger.ac.uk jc17@sanger.ac.uk neh@sanger.ac.uk,jc17@sanger.ac.uk neh@sanger.ac.uk,neh@sanger.ac.uk,hr1@sanger.ac.uk,,0,0,8381745,0,Hc_10_BC4_P2_5779_340285 7809258,Haemonchus contortus,6289,S0702,714,Haemonchus contortus Ivermectin Resistance Genomics Study,standard,,5707612,from:400 to:550,ERS323819,,Haemonchus contortus,,,25-30 mixed male and female worms%2C strain identity was checked using a panel of 4 microsatellite loci that discriminate Haemonchus contortus strains,,1660680,,Hc_10_BC4_P2_5779_340285,Haemonchus contortus MHco3%2F10.BC4(P2)-5779,Haemonchus_contortus (V1_21June13),,168,ERP000430,,0,0,Two H. contortus ivermectin resistance strains have been backcrossed 4 times against the susceptible genome strain. Parental strains and backcross strains will be sequenced in order to identify regions of the genome linked to ivermectin resistance-conferring loci.,1697,Haemonchus contortus Ivermectin Resistance,,,Haemonchus contortus Ivermectin Resistance,, +4,8215019,ERS351213,,TAAGGCGA,TAGATCGC,,qPCR only,TAAGGCGATAGATCGC,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell1 8215019,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351213,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706390,,mES_ai2_s2_cell1,mES_ai2_s2_cell1,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,1, +4,8215020,ERS351214,,CGTACTAG,TAGATCGC,,qPCR only,CGTACTAGTAGATCGC,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell2 8215020,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351214,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706391,,mES_ai2_s2_cell2,mES_ai2_s2_cell2,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,2, +4,8215021,ERS351221,,AGGCAGAA,TAGATCGC,,qPCR only,AGGCAGAATAGATCGC,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell3 8215021,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351221,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706392,,mES_ai2_s2_cell3,mES_ai2_s2_cell3,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,3, +4,8215022,ERS351222,,TCCTGAGC,TAGATCGC,,qPCR only,TCCTGAGCTAGATCGC,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell4 8215022,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351222,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706393,,mES_ai2_s2_cell4,mES_ai2_s2_cell4,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,4, +4,8215023,ERS351223,,GGACTCCT,TAGATCGC,,qPCR only,GGACTCCTTAGATCGC,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell5 8215023,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351223,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706394,,mES_ai2_s2_cell5,mES_ai2_s2_cell5,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,5, +4,8215024,ERS351224,,TAGGCATG,TAGATCGC,,qPCR only,TAGGCATGTAGATCGC,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell6 8215024,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351224,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706395,,mES_ai2_s2_cell6,mES_ai2_s2_cell6,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,6, +4,8215025,ERS351225,,CTCTCTAC,TAGATCGC,,qPCR only,CTCTCTACTAGATCGC,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell7 8215025,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351225,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706396,,mES_ai2_s2_cell7,mES_ai2_s2_cell7,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,7, +4,8215026,ERS351218,,CAGAGAGG,TAGATCGC,,qPCR only,CAGAGAGGTAGATCGC,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell8 8215026,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351218,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706397,,mES_ai2_s2_cell8,mES_ai2_s2_cell8,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,8, +4,8215027,ERS351219,,GCTACGCT,TAGATCGC,,qPCR only,GCTACGCTTAGATCGC,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell9 8215027,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351219,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706398,,mES_ai2_s2_cell9,mES_ai2_s2_cell9,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,9, +4,8215028,ERS351220,,CGAGGCTG,TAGATCGC,,qPCR only,CGAGGCTGTAGATCGC,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell10 8215028,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351220,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706399,,mES_ai2_s2_cell10,mES_ai2_s2_cell10,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,10, +4,8215029,ERS351235,,AAGAGGCA,TAGATCGC,,qPCR only,AAGAGGCATAGATCGC,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell11 8215029,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351235,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706400,,mES_ai2_s2_cell11,mES_ai2_s2_cell11,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,11, +4,8215030,ERS351237,,GTAGAGGA,TAGATCGC,,qPCR only,GTAGAGGATAGATCGC,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell12 8215030,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351237,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706401,,mES_ai2_s2_cell12,mES_ai2_s2_cell12,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,12, +4,8215031,ERS351238,,TAAGGCGA,CTCTCTAT,,qPCR only,TAAGGCGACTCTCTAT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell13 8215031,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351238,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706402,,mES_ai2_s2_cell13,mES_ai2_s2_cell13,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,13, +4,8215032,ERS351239,,CGTACTAG,CTCTCTAT,,qPCR only,CGTACTAGCTCTCTAT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell14 8215032,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351239,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706403,,mES_ai2_s2_cell14,mES_ai2_s2_cell14,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,14, +4,8215033,ERS351241,,AGGCAGAA,CTCTCTAT,,qPCR only,AGGCAGAACTCTCTAT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell15 8215033,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351241,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706404,,mES_ai2_s2_cell15,mES_ai2_s2_cell15,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,15, +4,8215034,ERS351226,,TCCTGAGC,CTCTCTAT,,qPCR only,TCCTGAGCCTCTCTAT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell16 8215034,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351226,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706405,,mES_ai2_s2_cell16,mES_ai2_s2_cell16,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,16, +4,8215035,ERS351227,,GGACTCCT,CTCTCTAT,,qPCR only,GGACTCCTCTCTCTAT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell17 8215035,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351227,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706406,,mES_ai2_s2_cell17,mES_ai2_s2_cell17,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,17, +4,8215036,ERS351234,,TAGGCATG,CTCTCTAT,,qPCR only,TAGGCATGCTCTCTAT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell18 8215036,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351234,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706407,,mES_ai2_s2_cell18,mES_ai2_s2_cell18,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,18, +4,8215037,ERS351236,,CTCTCTAC,CTCTCTAT,,qPCR only,CTCTCTACCTCTCTAT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell19 8215037,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351236,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706408,,mES_ai2_s2_cell19,mES_ai2_s2_cell19,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,19, +4,8215038,ERS351247,,CAGAGAGG,CTCTCTAT,,qPCR only,CAGAGAGGCTCTCTAT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell20 8215038,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351247,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706409,,mES_ai2_s2_cell20,mES_ai2_s2_cell20,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,20, +4,8215039,ERS351249,,GCTACGCT,CTCTCTAT,,qPCR only,GCTACGCTCTCTCTAT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell21 8215039,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351249,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706410,,mES_ai2_s2_cell21,mES_ai2_s2_cell21,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,21, +4,8215040,ERS351251,,CGAGGCTG,CTCTCTAT,,qPCR only,CGAGGCTGCTCTCTAT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell22 8215040,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351251,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706411,,mES_ai2_s2_cell22,mES_ai2_s2_cell22,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,22, +4,8215041,ERS351252,,AAGAGGCA,CTCTCTAT,,qPCR only,AAGAGGCACTCTCTAT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell23 8215041,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351252,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706412,,mES_ai2_s2_cell23,mES_ai2_s2_cell23,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,23, +4,8215042,ERS351242,,GTAGAGGA,CTCTCTAT,,qPCR only,GTAGAGGACTCTCTAT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell24 8215042,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351242,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706413,,mES_ai2_s2_cell24,mES_ai2_s2_cell24,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,24, +4,8215043,ERS351243,,TAAGGCGA,TATCCTCT,,qPCR only,TAAGGCGATATCCTCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell25 8215043,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351243,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706414,,mES_ai2_s2_cell25,mES_ai2_s2_cell25,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,25, +4,8215044,ERS351244,,CGTACTAG,TATCCTCT,,qPCR only,CGTACTAGTATCCTCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell26 8215044,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351244,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706415,,mES_ai2_s2_cell26,mES_ai2_s2_cell26,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,26, +4,8215045,ERS351245,,AGGCAGAA,TATCCTCT,,qPCR only,AGGCAGAATATCCTCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell27 8215045,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351245,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706416,,mES_ai2_s2_cell27,mES_ai2_s2_cell27,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,27, +4,8215046,ERS351248,,TCCTGAGC,TATCCTCT,,qPCR only,TCCTGAGCTATCCTCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell28 8215046,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351248,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706417,,mES_ai2_s2_cell28,mES_ai2_s2_cell28,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,28, +4,8215047,ERS351250,,GGACTCCT,TATCCTCT,,qPCR only,GGACTCCTTATCCTCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell29 8215047,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351250,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706418,,mES_ai2_s2_cell29,mES_ai2_s2_cell29,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,29, +4,8215048,ERS351260,,TAGGCATG,TATCCTCT,,qPCR only,TAGGCATGTATCCTCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell30 8215048,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351260,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706419,,mES_ai2_s2_cell30,mES_ai2_s2_cell30,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,30, +4,8215049,ERS351261,,CTCTCTAC,TATCCTCT,,qPCR only,CTCTCTACTATCCTCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell31 8215049,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351261,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706420,,mES_ai2_s2_cell31,mES_ai2_s2_cell31,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,31, +4,8215050,ERS351254,,CAGAGAGG,TATCCTCT,,qPCR only,CAGAGAGGTATCCTCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell32 8215050,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351254,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706421,,mES_ai2_s2_cell32,mES_ai2_s2_cell32,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,32, +4,8215051,ERS351255,,GCTACGCT,TATCCTCT,,qPCR only,GCTACGCTTATCCTCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell33 8215051,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351255,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706422,,mES_ai2_s2_cell33,mES_ai2_s2_cell33,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,33, +4,8215052,ERS351256,,CGAGGCTG,TATCCTCT,,qPCR only,CGAGGCTGTATCCTCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell34 8215052,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351256,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706423,,mES_ai2_s2_cell34,mES_ai2_s2_cell34,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,34, +4,8215053,ERS351257,,AAGAGGCA,TATCCTCT,,qPCR only,AAGAGGCATATCCTCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell35 8215053,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351257,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706424,,mES_ai2_s2_cell35,mES_ai2_s2_cell35,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,35, +4,8215054,ERS351258,,GTAGAGGA,TATCCTCT,,qPCR only,GTAGAGGATATCCTCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell36 8215054,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351258,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706425,,mES_ai2_s2_cell36,mES_ai2_s2_cell36,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,36, +4,8215055,ERS351259,,TAAGGCGA,AGAGTAGA,,qPCR only,TAAGGCGAAGAGTAGA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell37 8215055,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351259,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706426,,mES_ai2_s2_cell37,mES_ai2_s2_cell37,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,37, +4,8215056,ERS351269,,CGTACTAG,AGAGTAGA,,qPCR only,CGTACTAGAGAGTAGA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell38 8215056,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351269,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706427,,mES_ai2_s2_cell38,mES_ai2_s2_cell38,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,38, +4,8215057,ERS351271,,AGGCAGAA,AGAGTAGA,,qPCR only,AGGCAGAAAGAGTAGA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell39 8215057,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351271,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706428,,mES_ai2_s2_cell39,mES_ai2_s2_cell39,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,39, +4,8215058,ERS351262,,TCCTGAGC,AGAGTAGA,,qPCR only,TCCTGAGCAGAGTAGA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell40 8215058,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351262,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706429,,mES_ai2_s2_cell40,mES_ai2_s2_cell40,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,40, +4,8215059,ERS351263,,GGACTCCT,AGAGTAGA,,qPCR only,GGACTCCTAGAGTAGA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell41 8215059,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351263,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706430,,mES_ai2_s2_cell41,mES_ai2_s2_cell41,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,41, +4,8215060,ERS351264,,TAGGCATG,AGAGTAGA,,qPCR only,TAGGCATGAGAGTAGA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell42 8215060,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351264,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706431,,mES_ai2_s2_cell42,mES_ai2_s2_cell42,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,42, +4,8215061,ERS351266,,CTCTCTAC,AGAGTAGA,,qPCR only,CTCTCTACAGAGTAGA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell43 8215061,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351266,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706432,,mES_ai2_s2_cell43,mES_ai2_s2_cell43,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,43, +4,8215062,ERS351267,,CAGAGAGG,AGAGTAGA,,qPCR only,CAGAGAGGAGAGTAGA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell44 8215062,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351267,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706433,,mES_ai2_s2_cell44,mES_ai2_s2_cell44,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,44, +4,8215063,ERS351268,,GCTACGCT,AGAGTAGA,,qPCR only,GCTACGCTAGAGTAGA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell45 8215063,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351268,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706434,,mES_ai2_s2_cell45,mES_ai2_s2_cell45,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,45, +4,8215064,ERS351270,,CGAGGCTG,AGAGTAGA,,qPCR only,CGAGGCTGAGAGTAGA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell46 8215064,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351270,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706435,,mES_ai2_s2_cell46,mES_ai2_s2_cell46,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,46, +4,8215065,ERS351272,,AAGAGGCA,AGAGTAGA,,qPCR only,AAGAGGCAAGAGTAGA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell47 8215065,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351272,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706436,,mES_ai2_s2_cell47,mES_ai2_s2_cell47,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,47, +4,8215066,ERS351273,,GTAGAGGA,AGAGTAGA,,qPCR only,GTAGAGGAAGAGTAGA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell48 8215066,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351273,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706437,,mES_ai2_s2_cell48,mES_ai2_s2_cell48,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,48, +4,8215067,ERS351275,,TAAGGCGA,GTAAGGAG,,qPCR only,TAAGGCGAGTAAGGAG,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell49 8215067,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351275,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706438,,mES_ai2_s2_cell49,mES_ai2_s2_cell49,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,49, +4,8215068,ERS351277,,CGTACTAG,GTAAGGAG,,qPCR only,CGTACTAGGTAAGGAG,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell50 8215068,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351277,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706439,,mES_ai2_s2_cell50,mES_ai2_s2_cell50,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,50, +4,8215069,ERS351278,,AGGCAGAA,GTAAGGAG,,qPCR only,AGGCAGAAGTAAGGAG,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell51 8215069,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351278,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706440,,mES_ai2_s2_cell51,mES_ai2_s2_cell51,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,51, +4,8215070,ERS351279,,TCCTGAGC,GTAAGGAG,,qPCR only,TCCTGAGCGTAAGGAG,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell52 8215070,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351279,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706441,,mES_ai2_s2_cell52,mES_ai2_s2_cell52,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,52, +4,8215071,ERS351280,,GGACTCCT,GTAAGGAG,,qPCR only,GGACTCCTGTAAGGAG,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell53 8215071,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351280,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706442,,mES_ai2_s2_cell53,mES_ai2_s2_cell53,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,53, +4,8215072,ERS351281,,TAGGCATG,GTAAGGAG,,qPCR only,TAGGCATGGTAAGGAG,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell54 8215072,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351281,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706443,,mES_ai2_s2_cell54,mES_ai2_s2_cell54,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,54, +4,8215073,ERS351282,,CTCTCTAC,GTAAGGAG,,qPCR only,CTCTCTACGTAAGGAG,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell55 8215073,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351282,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706444,,mES_ai2_s2_cell55,mES_ai2_s2_cell55,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,55, +4,8215074,ERS351274,,CAGAGAGG,GTAAGGAG,,qPCR only,CAGAGAGGGTAAGGAG,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell56 8215074,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351274,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706445,,mES_ai2_s2_cell56,mES_ai2_s2_cell56,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,56, +4,8215075,ERS351276,,GCTACGCT,GTAAGGAG,,qPCR only,GCTACGCTGTAAGGAG,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell57 8215075,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351276,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706446,,mES_ai2_s2_cell57,mES_ai2_s2_cell57,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,57, +4,8215076,ERS351285,,CGAGGCTG,GTAAGGAG,,qPCR only,CGAGGCTGGTAAGGAG,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell58 8215076,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351285,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706447,,mES_ai2_s2_cell58,mES_ai2_s2_cell58,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,58, +4,8215077,ERS351286,,AAGAGGCA,GTAAGGAG,,qPCR only,AAGAGGCAGTAAGGAG,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell59 8215077,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351286,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706448,,mES_ai2_s2_cell59,mES_ai2_s2_cell59,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,59, +4,8215078,ERS351287,,GTAGAGGA,GTAAGGAG,,qPCR only,GTAGAGGAGTAAGGAG,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell60 8215078,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351287,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706449,,mES_ai2_s2_cell60,mES_ai2_s2_cell60,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,60, +4,8215079,ERS351288,,TAAGGCGA,ACTGCATA,,qPCR only,TAAGGCGAACTGCATA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell61 8215079,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351288,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706450,,mES_ai2_s2_cell61,mES_ai2_s2_cell61,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,61, +4,8215080,ERS351289,,CGTACTAG,ACTGCATA,,qPCR only,CGTACTAGACTGCATA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell62 8215080,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351289,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706451,,mES_ai2_s2_cell62,mES_ai2_s2_cell62,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,62, +4,8215081,ERS351290,,AGGCAGAA,ACTGCATA,,qPCR only,AGGCAGAAACTGCATA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell63 8215081,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351290,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706452,,mES_ai2_s2_cell63,mES_ai2_s2_cell63,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,63, +4,8215082,ERS351283,,TCCTGAGC,ACTGCATA,,qPCR only,TCCTGAGCACTGCATA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell64 8215082,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351283,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706453,,mES_ai2_s2_cell64,mES_ai2_s2_cell64,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,64, +4,8215083,ERS351284,,GGACTCCT,ACTGCATA,,qPCR only,GGACTCCTACTGCATA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell65 8215083,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351284,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706454,,mES_ai2_s2_cell65,mES_ai2_s2_cell65,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,65, +4,8215084,ERS351292,,TAGGCATG,ACTGCATA,,qPCR only,TAGGCATGACTGCATA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell66 8215084,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351292,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706455,,mES_ai2_s2_cell66,mES_ai2_s2_cell66,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,66, +4,8215085,ERS351293,,CTCTCTAC,ACTGCATA,,qPCR only,CTCTCTACACTGCATA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell67 8215085,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351293,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706456,,mES_ai2_s2_cell67,mES_ai2_s2_cell67,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,67, +4,8215086,ERS351294,,CAGAGAGG,ACTGCATA,,qPCR only,CAGAGAGGACTGCATA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell68 8215086,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351294,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706457,,mES_ai2_s2_cell68,mES_ai2_s2_cell68,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,68, +4,8215087,ERS351295,,GCTACGCT,ACTGCATA,,qPCR only,GCTACGCTACTGCATA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell69 8215087,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351295,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706458,,mES_ai2_s2_cell69,mES_ai2_s2_cell69,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,69, +4,8215088,ERS351296,,CGAGGCTG,ACTGCATA,,qPCR only,CGAGGCTGACTGCATA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell70 8215088,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351296,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706459,,mES_ai2_s2_cell70,mES_ai2_s2_cell70,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,70, +4,8215089,ERS351297,,AAGAGGCA,ACTGCATA,,qPCR only,AAGAGGCAACTGCATA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell71 8215089,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351297,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706460,,mES_ai2_s2_cell71,mES_ai2_s2_cell71,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,71, +4,8215090,ERS351291,,GTAGAGGA,ACTGCATA,,qPCR only,GTAGAGGAACTGCATA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell72 8215090,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351291,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706461,,mES_ai2_s2_cell72,mES_ai2_s2_cell72,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,72, +4,8215091,ERS351299,,TAAGGCGA,AAGGAGTA,,qPCR only,TAAGGCGAAAGGAGTA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell73 8215091,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351299,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706462,,mES_ai2_s2_cell73,mES_ai2_s2_cell73,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,73, +4,8215092,ERS351301,,CGTACTAG,AAGGAGTA,,qPCR only,CGTACTAGAAGGAGTA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell74 8215092,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351301,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706463,,mES_ai2_s2_cell74,mES_ai2_s2_cell74,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,74, +4,8215093,ERS351302,,AGGCAGAA,AAGGAGTA,,qPCR only,AGGCAGAAAAGGAGTA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell75 8215093,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351302,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706464,,mES_ai2_s2_cell75,mES_ai2_s2_cell75,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,75, +4,8215094,ERS351303,,TCCTGAGC,AAGGAGTA,,qPCR only,TCCTGAGCAAGGAGTA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell76 8215094,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351303,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706465,,mES_ai2_s2_cell76,mES_ai2_s2_cell76,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,76, +4,8215095,ERS351304,,GGACTCCT,AAGGAGTA,,qPCR only,GGACTCCTAAGGAGTA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell77 8215095,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351304,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706466,,mES_ai2_s2_cell77,mES_ai2_s2_cell77,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,77, +4,8215096,ERS351305,,TAGGCATG,AAGGAGTA,,qPCR only,TAGGCATGAAGGAGTA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell78 8215096,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351305,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706467,,mES_ai2_s2_cell78,mES_ai2_s2_cell78,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,78, +4,8215097,ERS351306,,CTCTCTAC,AAGGAGTA,,qPCR only,CTCTCTACAAGGAGTA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell79 8215097,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351306,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706468,,mES_ai2_s2_cell79,mES_ai2_s2_cell79,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,79, +4,8215098,ERS351298,,CAGAGAGG,AAGGAGTA,,qPCR only,CAGAGAGGAAGGAGTA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell80 8215098,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351298,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706469,,mES_ai2_s2_cell80,mES_ai2_s2_cell80,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,80, +4,8215099,ERS351300,,GCTACGCT,AAGGAGTA,,qPCR only,GCTACGCTAAGGAGTA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell81 8215099,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351300,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706470,,mES_ai2_s2_cell81,mES_ai2_s2_cell81,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,81, +4,8215100,ERS351309,,CGAGGCTG,AAGGAGTA,,qPCR only,CGAGGCTGAAGGAGTA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell82 8215100,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351309,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706471,,mES_ai2_s2_cell82,mES_ai2_s2_cell82,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,82, +4,8215101,ERS351310,,AAGAGGCA,AAGGAGTA,,qPCR only,AAGAGGCAAAGGAGTA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell83 8215101,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351310,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706472,,mES_ai2_s2_cell83,mES_ai2_s2_cell83,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,83, +4,8215102,ERS351311,,GTAGAGGA,AAGGAGTA,,qPCR only,GTAGAGGAAAGGAGTA,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell84 8215102,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351311,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706473,,mES_ai2_s2_cell84,mES_ai2_s2_cell84,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,84, +4,8215103,ERS351312,,TAAGGCGA,CTAAGCCT,,qPCR only,TAAGGCGACTAAGCCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell85 8215103,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351312,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706474,,mES_ai2_s2_cell85,mES_ai2_s2_cell85,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,85, +4,8215104,ERS351313,,CGTACTAG,CTAAGCCT,,qPCR only,CGTACTAGCTAAGCCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell86 8215104,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351313,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706475,,mES_ai2_s2_cell86,mES_ai2_s2_cell86,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,86, +4,8215105,ERS351314,,AGGCAGAA,CTAAGCCT,,qPCR only,AGGCAGAACTAAGCCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell87 8215105,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351314,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706476,,mES_ai2_s2_cell87,mES_ai2_s2_cell87,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,87, +4,8215106,ERS351307,,TCCTGAGC,CTAAGCCT,,qPCR only,TCCTGAGCCTAAGCCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell88 8215106,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351307,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706477,,mES_ai2_s2_cell88,mES_ai2_s2_cell88,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,88, +4,8215107,ERS351308,,GGACTCCT,CTAAGCCT,,qPCR only,GGACTCCTCTAAGCCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell89 8215107,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351308,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706478,,mES_ai2_s2_cell89,mES_ai2_s2_cell89,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,89, +4,8215108,ERS351315,,TAGGCATG,CTAAGCCT,,qPCR only,TAGGCATGCTAAGCCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell90 8215108,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351315,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706479,,mES_ai2_s2_cell90,mES_ai2_s2_cell90,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,90, +4,8215109,ERS351316,,CTCTCTAC,CTAAGCCT,,qPCR only,CTCTCTACCTAAGCCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell91 8215109,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351316,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706480,,mES_ai2_s2_cell91,mES_ai2_s2_cell91,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,91, +4,8215110,ERS351317,,CAGAGAGG,CTAAGCCT,,qPCR only,CAGAGAGGCTAAGCCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell92 8215110,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351317,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706481,,mES_ai2_s2_cell92,mES_ai2_s2_cell92,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,92, +4,8215111,ERS351318,,GCTACGCT,CTAAGCCT,,qPCR only,GCTACGCTCTAAGCCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell93 8215111,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351318,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706482,,mES_ai2_s2_cell93,mES_ai2_s2_cell93,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,93, +4,8215112,ERS351319,,CGAGGCTG,CTAAGCCT,,qPCR only,CGAGGCTGCTAAGCCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell94 8215112,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351319,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706483,,mES_ai2_s2_cell94,mES_ai2_s2_cell94,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,94, +4,8215113,ERS351320,,AAGAGGCA,CTAAGCCT,,qPCR only,AAGAGGCACTAAGCCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell95 8215113,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351320,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706484,,mES_ai2_s2_cell95,mES_ai2_s2_cell95,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,95, +4,8215114,ERS351321,,GTAGAGGA,CTAAGCCT,,qPCR only,GTAGAGGACTAAGCCT,,ncb@sanger.ac.uk ola@ebi.ac.uk,,ncb@sanger.ac.uk,ola@ebi.ac.uk,,0,0,,,mES_ai2_s2_cell96 8215114,mus musculus,10090,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:300 to:1000,ERS351321,,Mus Musculus,,,mRNAseq Nextera library made from G4 ES cell line from a male blastocyst from a C57BL%2F6Ncr male x 129S6%2FSvEvTac female,,1706485,,mES_ai2_s2_cell96,mES_ai2_s2_cell96,Mus_musculus (NCBIm37),,168,ERP003293,1,0,0,This study aims to investigate the transcriptomic heterogeneity of mouse embryonic stem cells (mES cells) in different culturing conditions with single-cell mRNA-seq technology. Previous studies have reported heterogeneous and differential allelic expression of specific pluripotency genes%2C e.g. Nanog%2C in mES cells in different culturing conditions. The fluctuation of gene expression has been linked to drift of pluripotent state. In this study%2C we will dissect the transcriptome of mouse ES cells culturing in conventiaonal serum%2FLIF and 2i%2FLIF conditions at the single-cell level. The usage of a hybrid mouse ES cell line in this study provides additional insight into differential allelic expression in these two culturing conditions. The data generated in this study will shed light on the regulation of pluripotency network.%0A ,2658,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,Mus_musculus (NCBIm37),,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency ,96, +4,6200285,phiX_for_spiked_buffers,,ACAACGCAAT,,,,ACAACGCAAT,,hps@sanger.ac.uk,,hps@sanger.ac.uk,hps@sanger.ac.uk,,1,0,,,PhiX_PS_20120919,,,,,,standard,,,,,,,,,,,1255141,,phiX_for_spiked_buffers,,,,168,,1,0,0,None,198,Illumina Controls,,,,168, +5,8269740,EGAN00001173643,,,,,No PCR,,,cdt@sanger.ac.uk cs4@sanger.ac.uk je6@sanger.ac.uk las@sanger.ac.uk lm5@sanger.ac.uk sm2@sanger.ac.uk som@sanger.ac.uk,cdt@sanger.ac.uk je6@sanger.ac.uk las@sanger.ac.uk som@sanger.ac.uk,cdt@sanger.ac.uk cs4@sanger.ac.uk lm5@sanger.ac.uk sm2@sanger.ac.uk,sm2@sanger.ac.uk,,0,0,8381740,0,PD14393b_wg 8269740,Human,9606,S0814,1422,CGP Core Sequencing 10%2F13 to 09%2F14,standard,,5676016,from:300 to:500,EGAN00001173643,,Homo sapiens,,,,,1712041,,PD14393b_wg,PD14393b,,,168,EGAS00001000290,1,0,0,Wholegenome libraries will be prepared from at least two serial samples reflecting different stages of disease progression and matched constitutional DNA for 30 Myeloproliferative Disease samples. Five lanes of Illumina HiSeq sequencing will be performed on each of the tumour samples and four lanes for each of the constitutional DNA. Sequencing data will mapped to build 37 of the human reference genome and analysis will be performed to characterize the spectrum of somatic variation present in these samples including single base pair mutations%2C insertions%2C deletions as well as larger structural variants and genomic rearrangements. ,2239,MPN Whole Genomes,Homo_sapiens (CGP_GRCh37.NCBI.allchr_MT),,Myeloproliferative Disease Whole Genomes,, +6,8324592,ERS354532,,GAGATTCC,TAATCTTA,,Pre-quality controlled,GAGATTCCTAATCTTA,,ms27@sanger.ac.uk ncb@sanger.ac.uk,,ncb@sanger.ac.uk,ms27@sanger.ac.uk,,0,0,,,T_BCM1_F4 8324592,Mouse,10090,S1553,1238,Mouse model to quantify genotype-epigenotype variations,standard,,,from:100 to:1000,ERS354532,,Mus musculus,,,RNA,,1694494,,T_BCM1_F4,RT_37,,,168,ERP002223,1,0,0,In order to examine the impact of genetic variation on epigenetic marking and control of gene expression%2C this study has been initiated using two inbred strains of mice%2C C57BL%2F6J and CAST%2FEij which genomes indicate substantial structural and nucleotide variation. Stocks of adult C57BL%2F6J and CAST%2FEij mice as well as CAST%2FBL6 and BL6%2FCAST reciprocal hybrids are employed for isolation of pure and synchronous populations of B and T-lymphocytes. RNA-Seq expression analysis will be performed in total RNA extracted from B-lymphocytes isolated from males and females of both strains and hybrids.%0A %0AThis data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria)%2C please see http%3A%2F%2Fwww.sanger.ac.uk%2Fdatasharing%2F,2501,Mouse model to quantify genotype-epigenotype variations_RNA,Mus_musculus (GRCm38),,Mouse model to quantify genotype-epigenotype variations ,64, +6,8324593,ERS354533,,ATTCAGAA,TAATCTTA,,Pre-quality controlled,ATTCAGAATAATCTTA,,ms27@sanger.ac.uk ncb@sanger.ac.uk,,ncb@sanger.ac.uk,ms27@sanger.ac.uk,,0,0,,,T_BCM1_F5 8324593,Mouse,10090,S1553,1238,Mouse model to quantify genotype-epigenotype variations,standard,,,from:100 to:1000,ERS354533,,Mus musculus,,,RNA,,1694495,,T_BCM1_F5,RT_38,,,168,ERP002223,1,0,0,In order to examine the impact of genetic variation on epigenetic marking and control of gene expression%2C this study has been initiated using two inbred strains of mice%2C C57BL%2F6J and CAST%2FEij which genomes indicate substantial structural and nucleotide variation. Stocks of adult C57BL%2F6J and CAST%2FEij mice as well as CAST%2FBL6 and BL6%2FCAST reciprocal hybrids are employed for isolation of pure and synchronous populations of B and T-lymphocytes. RNA-Seq expression analysis will be performed in total RNA extracted from B-lymphocytes isolated from males and females of both strains and hybrids.%0A %0AThis data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria)%2C please see http%3A%2F%2Fwww.sanger.ac.uk%2Fdatasharing%2F,2501,Mouse model to quantify genotype-epigenotype variations_RNA,Mus_musculus (GRCm38),,Mouse model to quantify genotype-epigenotype variations ,65, +6,6200285,phiX_for_spiked_buffers,,ACAACGCAAT,,,,ACAACGCAAT,,hps@sanger.ac.uk,,hps@sanger.ac.uk,hps@sanger.ac.uk,,1,0,,,PhiX_PS_20120919,,,,,,standard,,,,,,,,,,,1255141,,phiX_for_spiked_buffers,,,,168,,1,0,0,None,198,Illumina Controls,,,,168, +7,8324594,ERS354534,,GAGATTCC,CAGGACGT,,Pre-quality controlled,GAGATTCCCAGGACGT,,ms27@sanger.ac.uk ncb@sanger.ac.uk,,ncb@sanger.ac.uk,ms27@sanger.ac.uk,,0,0,,,T_CBF1_G4 8324594,Mouse,10090,S1553,1238,Mouse model to quantify genotype-epigenotype variations,standard,,,from:100 to:1000,ERS354534,,Mus musculus,,,RNA,,1694496,,T_CBF1_G4,RT_39,,,168,ERP002223,1,0,0,In order to examine the impact of genetic variation on epigenetic marking and control of gene expression%2C this study has been initiated using two inbred strains of mice%2C C57BL%2F6J and CAST%2FEij which genomes indicate substantial structural and nucleotide variation. Stocks of adult C57BL%2F6J and CAST%2FEij mice as well as CAST%2FBL6 and BL6%2FCAST reciprocal hybrids are employed for isolation of pure and synchronous populations of B and T-lymphocytes. RNA-Seq expression analysis will be performed in total RNA extracted from B-lymphocytes isolated from males and females of both strains and hybrids.%0A %0AThis data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria)%2C please see http%3A%2F%2Fwww.sanger.ac.uk%2Fdatasharing%2F,2501,Mouse model to quantify genotype-epigenotype variations_RNA,Mus_musculus (GRCm38),,Mouse model to quantify genotype-epigenotype variations ,76, +7,8324595,ERS354535,,ATTCAGAA,CAGGACGT,,Pre-quality controlled,ATTCAGAACAGGACGT,,ms27@sanger.ac.uk ncb@sanger.ac.uk,,ncb@sanger.ac.uk,ms27@sanger.ac.uk,,0,0,,,T_CBF1_G5 8324595,Mouse,10090,S1553,1238,Mouse model to quantify genotype-epigenotype variations,standard,,,from:100 to:1000,ERS354535,,Mus musculus,,,RNA,,1694497,,T_CBF1_G5,RT_40,,,168,ERP002223,1,0,0,In order to examine the impact of genetic variation on epigenetic marking and control of gene expression%2C this study has been initiated using two inbred strains of mice%2C C57BL%2F6J and CAST%2FEij which genomes indicate substantial structural and nucleotide variation. Stocks of adult C57BL%2F6J and CAST%2FEij mice as well as CAST%2FBL6 and BL6%2FCAST reciprocal hybrids are employed for isolation of pure and synchronous populations of B and T-lymphocytes. RNA-Seq expression analysis will be performed in total RNA extracted from B-lymphocytes isolated from males and females of both strains and hybrids.%0A %0AThis data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria)%2C please see http%3A%2F%2Fwww.sanger.ac.uk%2Fdatasharing%2F,2501,Mouse model to quantify genotype-epigenotype variations_RNA,Mus_musculus (GRCm38),,Mouse model to quantify genotype-epigenotype variations ,77, +7,6200285,phiX_for_spiked_buffers,,ACAACGCAAT,,,,ACAACGCAAT,,hps@sanger.ac.uk,,hps@sanger.ac.uk,hps@sanger.ac.uk,,1,0,,,PhiX_PS_20120919,,,,,,standard,,,,,,,,,,,1255141,,phiX_for_spiked_buffers,,,,168,,1,0,0,None,198,Illumina Controls,,,,168, +8,8324596,ERS354536,,GAGATTCC,GTACTGAC,,Pre-quality controlled,GAGATTCCGTACTGAC,,ms27@sanger.ac.uk ncb@sanger.ac.uk,,ncb@sanger.ac.uk,ms27@sanger.ac.uk,,0,0,,,T_CBM2_H4 8324596,Mouse,10090,S1553,1238,Mouse model to quantify genotype-epigenotype variations,standard,,,from:100 to:1000,ERS354536,,Mus musculus,,,RNA,,1694498,,T_CBM2_H4,RT_41,,,168,ERP002223,1,0,0,In order to examine the impact of genetic variation on epigenetic marking and control of gene expression%2C this study has been initiated using two inbred strains of mice%2C C57BL%2F6J and CAST%2FEij which genomes indicate substantial structural and nucleotide variation. Stocks of adult C57BL%2F6J and CAST%2FEij mice as well as CAST%2FBL6 and BL6%2FCAST reciprocal hybrids are employed for isolation of pure and synchronous populations of B and T-lymphocytes. RNA-Seq expression analysis will be performed in total RNA extracted from B-lymphocytes isolated from males and females of both strains and hybrids.%0A %0AThis data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria)%2C please see http%3A%2F%2Fwww.sanger.ac.uk%2Fdatasharing%2F,2501,Mouse model to quantify genotype-epigenotype variations_RNA,Mus_musculus (GRCm38),,Mouse model to quantify genotype-epigenotype variations ,88, +8,8324597,ERS354537,,ATTCAGAA,GTACTGAC,,Pre-quality controlled,ATTCAGAAGTACTGAC,,ms27@sanger.ac.uk ncb@sanger.ac.uk,,ncb@sanger.ac.uk,ms27@sanger.ac.uk,,0,0,,,T_CBM2_H5 8324597,Mouse,10090,S1553,1238,Mouse model to quantify genotype-epigenotype variations,standard,,,from:100 to:1000,ERS354537,,Mus musculus,,,RNA,,1694499,,T_CBM2_H5,RT_42,,,168,ERP002223,1,0,0,In order to examine the impact of genetic variation on epigenetic marking and control of gene expression%2C this study has been initiated using two inbred strains of mice%2C C57BL%2F6J and CAST%2FEij which genomes indicate substantial structural and nucleotide variation. Stocks of adult C57BL%2F6J and CAST%2FEij mice as well as CAST%2FBL6 and BL6%2FCAST reciprocal hybrids are employed for isolation of pure and synchronous populations of B and T-lymphocytes. RNA-Seq expression analysis will be performed in total RNA extracted from B-lymphocytes isolated from males and females of both strains and hybrids.%0A %0AThis data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria)%2C please see http%3A%2F%2Fwww.sanger.ac.uk%2Fdatasharing%2F,2501,Mouse model to quantify genotype-epigenotype variations_RNA,Mus_musculus (GRCm38),,Mouse model to quantify genotype-epigenotype variations ,89, +8,6200285,phiX_for_spiked_buffers,,ACAACGCAAT,,,,ACAACGCAAT,,hps@sanger.ac.uk,,hps@sanger.ac.uk,hps@sanger.ac.uk,,1,0,,,PhiX_PS_20120919,,,,,,standard,,,,,,,,,,,1255141,,phiX_for_spiked_buffers,,,,168,,1,0,0,None,198,Illumina Controls,,,,168, diff --git a/t/data/samplesheet/6946_extended.csv b/t/data/samplesheet/6946_extended.csv index 30532ec0..74765147 100644 --- a/t/data/samplesheet/6946_extended.csv +++ b/t/data/samplesheet/6946_extended.csv @@ -1,14 +1,14 @@ -[Data],,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, -Sample_ID,Sample_Name,GenomeFolder,Index,bait_name,default_library_type,default_tag_sequence,default_tagtwo_sequence,email_addresses,email_addresses_of_followers,email_addresses_of_managers,email_addresses_of_owners,gbs_plex_name,is_control,is_pool,lane_id,lane_priority,library_name,organism,organism_taxon_id,project_cost_code,project_id,project_name,purpose,qc_state,request_id,required_insert_size_range,sample_accession_number,sample_cohort,sample_common_name,sample_consent_withdrawn,sample_description,sample_donor_id,sample_id,sample_name,sample_public_name,sample_reference_genome,sample_supplier_name,spiked_phix_tag_index,study_accession_number,study_alignments_in_bam,study_contains_nonconsented_human,study_contains_nonconsented_xahuman,study_description,study_id,study_name,study_reference_genome,study_separate_y_chromosome_data,study_title,tag_index, -3789278,Salmonella pullorum,,ATCACGTT,,Standard,ATCACGTT,,ems@sanger.ac.uk mq1@sanger.ac.uk,,ems@sanger.ac.uk mq1@sanger.ac.uk,mq1@sanger.ac.uk,,0,0,,,sp200shear 3789278,Salmonella pullorum,590,S0696,678,MQ R and D,standard,,,from:200 to:700,,,Salmonella pullorum,,genomic DNA,,1289832,sp200shear,Salmonella pullorum,Salmonella_pullorum (449_87),,,,1,0,0,- I have agreed to alpha test the kapa hifi qPCR kit. This allows prep PCR to be followed in real time and stopped when sufficient product has accumulated so preventing overcycling and allowing user to interrogate prep PCR step. %09%09%09%09%09%0A- If we are to use this enzyme we need to know that it is at least as good as Phusion in terms of fidelity and coverage.%09%09%09%09%09%0A- In theory kapa hifi has higher fidelity than phusion.%09%09%09%09%09,700,Kapa HiFi test,,,hifi test,1, -3789279,Bordetella Pertussis,,CGATGTTT,,Standard,CGATGTTT,,ems@sanger.ac.uk mq1@sanger.ac.uk,,ems@sanger.ac.uk mq1@sanger.ac.uk,mq1@sanger.ac.uk,,0,0,,,bp200shear 3789279,Bordetella Pertussis,520,S0696,678,MQ R and D,standard,,,from:200 to:700,,,Bordetella Pertussis,,genomic DNA,,1289833,bp200shear,Bordetella Pertussis,Bordetella_pertussis (ST24),,,,1,0,0,- I have agreed to alpha test the kapa hifi qPCR kit. This allows prep PCR to be followed in real time and stopped when sufficient product has accumulated so preventing overcycling and allowing user to interrogate prep PCR step. %09%09%09%09%09%0A- If we are to use this enzyme we need to know that it is at least as good as Phusion in terms of fidelity and coverage.%09%09%09%09%09%0A- In theory kapa hifi has higher fidelity than phusion.%09%09%09%09%09,700,Kapa HiFi test,,,hifi test,2, -3789280,Plasmodium Falciparum,,TTAGGCAT,,Standard,TTAGGCAT,,ems@sanger.ac.uk mq1@sanger.ac.uk,,ems@sanger.ac.uk mq1@sanger.ac.uk,mq1@sanger.ac.uk,,0,0,,,3D7200shear 3789280,Plasmodium Falciparum,5820,S0696,678,MQ R and D,standard,,,from:200 to:700,,,Plasmodium Falciparum,,genomic DNA,,1289834,3D7200shear,Plasmodium Falciparum,Plasmodium_falciparum (3D7),,,,1,0,0,- I have agreed to alpha test the kapa hifi qPCR kit. This allows prep PCR to be followed in real time and stopped when sufficient product has accumulated so preventing overcycling and allowing user to interrogate prep PCR step. %09%09%09%09%09%0A- If we are to use this enzyme we need to know that it is at least as good as Phusion in terms of fidelity and coverage.%09%09%09%09%09%0A- In theory kapa hifi has higher fidelity than phusion.%09%09%09%09%09,700,Kapa HiFi test,,,hifi test,3, -3789281,Homo sapiens,,TGACCACT,,Standard,TGACCACT,,ems@sanger.ac.uk mq1@sanger.ac.uk,,ems@sanger.ac.uk mq1@sanger.ac.uk,mq1@sanger.ac.uk,,0,0,,,human200shear 3789281,Homo sapiens,9606,S0696,678,MQ R and D,standard,,,from:200 to:700,,,Human,,genomic DNA,,1289835,human200shear,Homo sapiens,Homo_sapiens (GRCh37_53),,,,1,0,0,- I have agreed to alpha test the kapa hifi qPCR kit. This allows prep PCR to be followed in real time and stopped when sufficient product has accumulated so preventing overcycling and allowing user to interrogate prep PCR step. %09%09%09%09%09%0A- If we are to use this enzyme we need to know that it is at least as good as Phusion in terms of fidelity and coverage.%09%09%09%09%09%0A- In theory kapa hifi has higher fidelity than phusion.%09%09%09%09%09,700,Kapa HiFi test,,,hifi test,4, -3789282,Salmonella pullorum,,ACAGTGGT,,Standard,ACAGTGGT,,ems@sanger.ac.uk mq1@sanger.ac.uk,,ems@sanger.ac.uk mq1@sanger.ac.uk,mq1@sanger.ac.uk,,0,0,,,sp300shear 3789282,Salmonella pullorum,590,S0696,678,MQ R and D,standard,,,from:200 to:700,,,Salmonella pullorum,,genomic DNA,,1289836,sp300shear,Salmonella pullorum,Salmonella_pullorum (449_87),,,,1,0,0,- I have agreed to alpha test the kapa hifi qPCR kit. This allows prep PCR to be followed in real time and stopped when sufficient product has accumulated so preventing overcycling and allowing user to interrogate prep PCR step. %09%09%09%09%09%0A- If we are to use this enzyme we need to know that it is at least as good as Phusion in terms of fidelity and coverage.%09%09%09%09%09%0A- In theory kapa hifi has higher fidelity than phusion.%09%09%09%09%09,700,Kapa HiFi test,,,hifi test,5, -3789283,Bordetella Pertussis,,GCCAATGT,,Standard,GCCAATGT,,ems@sanger.ac.uk mq1@sanger.ac.uk,,ems@sanger.ac.uk mq1@sanger.ac.uk,mq1@sanger.ac.uk,,0,0,,,bp300shear 3789283,Bordetella Pertussis,520,S0696,678,MQ R and D,standard,,,from:200 to:700,,,Bordetella Pertussis,,genomic DNA,,1289837,bp300shear,Bordetella Pertussis,Bordetella_pertussis (ST24),,,,1,0,0,- I have agreed to alpha test the kapa hifi qPCR kit. This allows prep PCR to be followed in real time and stopped when sufficient product has accumulated so preventing overcycling and allowing user to interrogate prep PCR step. %09%09%09%09%09%0A- If we are to use this enzyme we need to know that it is at least as good as Phusion in terms of fidelity and coverage.%09%09%09%09%09%0A- In theory kapa hifi has higher fidelity than phusion.%09%09%09%09%09,700,Kapa HiFi test,,,hifi test,6, -3789284,Plasmodium Falciparum,,CAGATCTG,,Standard,CAGATCTG,,ems@sanger.ac.uk mq1@sanger.ac.uk,,ems@sanger.ac.uk mq1@sanger.ac.uk,mq1@sanger.ac.uk,,0,0,,,3D7300shear 3789284,Plasmodium Falciparum,5820,S0696,678,MQ R and D,standard,,,from:200 to:700,,,Plasmodium Falciparum,,genomic DNA,,1289838,3D7300shear,Plasmodium Falciparum,Plasmodium_falciparum (3D7),,,,1,0,0,- I have agreed to alpha test the kapa hifi qPCR kit. This allows prep PCR to be followed in real time and stopped when sufficient product has accumulated so preventing overcycling and allowing user to interrogate prep PCR step. %09%09%09%09%09%0A- If we are to use this enzyme we need to know that it is at least as good as Phusion in terms of fidelity and coverage.%09%09%09%09%09%0A- In theory kapa hifi has higher fidelity than phusion.%09%09%09%09%09,700,Kapa HiFi test,,,hifi test,7, -3789285,Homo sapiens,,ACTTGATG,,Standard,ACTTGATG,,ems@sanger.ac.uk mq1@sanger.ac.uk,,ems@sanger.ac.uk mq1@sanger.ac.uk,mq1@sanger.ac.uk,,0,0,,,human300shear 3789285,Homo sapiens,9606,S0696,678,MQ R and D,standard,,,from:200 to:700,,,Human,,genomic DNA,,1289839,human300shear,Homo sapiens,Homo_sapiens (GRCh37_53),,,,1,0,0,- I have agreed to alpha test the kapa hifi qPCR kit. This allows prep PCR to be followed in real time and stopped when sufficient product has accumulated so preventing overcycling and allowing user to interrogate prep PCR step. %09%09%09%09%09%0A- If we are to use this enzyme we need to know that it is at least as good as Phusion in terms of fidelity and coverage.%09%09%09%09%09%0A- In theory kapa hifi has higher fidelity than phusion.%09%09%09%09%09,700,Kapa HiFi test,,,hifi test,8, -3789286,Salmonella pullorum,,GATCAGCG,,Standard,GATCAGCG,,ems@sanger.ac.uk mq1@sanger.ac.uk,,ems@sanger.ac.uk mq1@sanger.ac.uk,mq1@sanger.ac.uk,,0,0,,,sp400shear 3789286,Salmonella pullorum,590,S0696,678,MQ R and D,standard,,,from:200 to:700,,,Salmonella pullorum,,genomic DNA,,1289840,sp400shear,Salmonella pullorum,Salmonella_pullorum (449_87),,,,1,0,0,- I have agreed to alpha test the kapa hifi qPCR kit. This allows prep PCR to be followed in real time and stopped when sufficient product has accumulated so preventing overcycling and allowing user to interrogate prep PCR step. %09%09%09%09%09%0A- If we are to use this enzyme we need to know that it is at least as good as Phusion in terms of fidelity and coverage.%09%09%09%09%09%0A- In theory kapa hifi has higher fidelity than phusion.%09%09%09%09%09,700,Kapa HiFi test,,,hifi test,9, -3789287,Bordetella Pertussis,,TAGCTTGT,,Standard,TAGCTTGT,,ems@sanger.ac.uk mq1@sanger.ac.uk,,ems@sanger.ac.uk mq1@sanger.ac.uk,mq1@sanger.ac.uk,,0,0,,,bp400shear 3789287,Bordetella Pertussis,520,S0696,678,MQ R and D,standard,,,from:200 to:700,,,Bordetella Pertussis,,genomic DNA,,1289841,bp400shear,Bordetella Pertussis,Bordetella_pertussis (ST24),,,,1,0,0,- I have agreed to alpha test the kapa hifi qPCR kit. This allows prep PCR to be followed in real time and stopped when sufficient product has accumulated so preventing overcycling and allowing user to interrogate prep PCR step. %09%09%09%09%09%0A- If we are to use this enzyme we need to know that it is at least as good as Phusion in terms of fidelity and coverage.%09%09%09%09%09%0A- In theory kapa hifi has higher fidelity than phusion.%09%09%09%09%09,700,Kapa HiFi test,,,hifi test,10, -3789288,Plasmodium Falciparum,,GGCTACAG,,Standard,GGCTACAG,,ems@sanger.ac.uk mq1@sanger.ac.uk,,ems@sanger.ac.uk mq1@sanger.ac.uk,mq1@sanger.ac.uk,,0,0,,,3D7400shear 3789288,Plasmodium Falciparum,5820,S0696,678,MQ R and D,standard,,,from:200 to:700,,,Plasmodium Falciparum,,genomic DNA,,1289842,3D7400shear,Plasmodium Falciparum,Plasmodium_falciparum (3D7),,,,1,0,0,- I have agreed to alpha test the kapa hifi qPCR kit. This allows prep PCR to be followed in real time and stopped when sufficient product has accumulated so preventing overcycling and allowing user to interrogate prep PCR step. %09%09%09%09%09%0A- If we are to use this enzyme we need to know that it is at least as good as Phusion in terms of fidelity and coverage.%09%09%09%09%09%0A- In theory kapa hifi has higher fidelity than phusion.%09%09%09%09%09,700,Kapa HiFi test,,,hifi test,11, -3789289,Homo sapiens,,CTTGTACT,,Standard,CTTGTACT,,ems@sanger.ac.uk mq1@sanger.ac.uk,,ems@sanger.ac.uk mq1@sanger.ac.uk,mq1@sanger.ac.uk,,0,0,,,human400shear 3789289,Homo sapiens,9606,S0696,678,MQ R and D,standard,,,from:200 to:700,,,Human,,genomic DNA,,1289843,human400shear,Homo sapiens,Homo_sapiens (GRCh37_53),,,,1,0,0,- I have agreed to alpha test the kapa hifi qPCR kit. This allows prep PCR to be followed in real time and stopped when sufficient product has accumulated so preventing overcycling and allowing user to interrogate prep PCR step. %09%09%09%09%09%0A- If we are to use this enzyme we need to know that it is at least as good as Phusion in terms of fidelity and coverage.%09%09%09%09%09%0A- In theory kapa hifi has higher fidelity than phusion.%09%09%09%09%09,700,Kapa HiFi test,,,hifi test,12, +[Data],,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +Sample_ID,Sample_Name,GenomeFolder,Index,bait_name,default_library_type,default_tag_sequence,default_tagtwo_sequence,email_addresses,email_addresses_of_followers,email_addresses_of_managers,email_addresses_of_owners,gbs_plex_name,is_control,is_pool,lane_id,lane_priority,library_name,organism,organism_taxon_id,project_cost_code,project_id,project_name,purpose,qc_state,request_id,required_insert_size_range,sample_accession_number,sample_cohort,sample_common_name,sample_consent_withdrawn,sample_control_type,sample_description,sample_donor_id,sample_id,sample_is_control,sample_name,sample_public_name,sample_reference_genome,sample_supplier_name,spiked_phix_tag_index,study_accession_number,study_alignments_in_bam,study_contains_nonconsented_human,study_contains_nonconsented_xahuman,study_description,study_id,study_name,study_reference_genome,study_separate_y_chromosome_data,study_title,tag_index, +3789278,Salmonella pullorum,,ATCACGTT,,Standard,ATCACGTT,,ems@sanger.ac.uk mq1@sanger.ac.uk,,ems@sanger.ac.uk mq1@sanger.ac.uk,mq1@sanger.ac.uk,,0,0,,,sp200shear 3789278,Salmonella pullorum,590,S0696,678,MQ R and D,standard,,,from:200 to:700,,,Salmonella pullorum,,,genomic DNA,,1289832,,sp200shear,Salmonella pullorum,Salmonella_pullorum (449_87),,,,1,0,0,- I have agreed to alpha test the kapa hifi qPCR kit. This allows prep PCR to be followed in real time and stopped when sufficient product has accumulated so preventing overcycling and allowing user to interrogate prep PCR step. %09%09%09%09%09%0A- If we are to use this enzyme we need to know that it is at least as good as Phusion in terms of fidelity and coverage.%09%09%09%09%09%0A- In theory kapa hifi has higher fidelity than phusion.%09%09%09%09%09,700,Kapa HiFi test,,,hifi test,1, +3789279,Bordetella Pertussis,,CGATGTTT,,Standard,CGATGTTT,,ems@sanger.ac.uk mq1@sanger.ac.uk,,ems@sanger.ac.uk mq1@sanger.ac.uk,mq1@sanger.ac.uk,,0,0,,,bp200shear 3789279,Bordetella Pertussis,520,S0696,678,MQ R and D,standard,,,from:200 to:700,,,Bordetella Pertussis,,,genomic DNA,,1289833,,bp200shear,Bordetella Pertussis,Bordetella_pertussis (ST24),,,,1,0,0,- I have agreed to alpha test the kapa hifi qPCR kit. This allows prep PCR to be followed in real time and stopped when sufficient product has accumulated so preventing overcycling and allowing user to interrogate prep PCR step. %09%09%09%09%09%0A- If we are to use this enzyme we need to know that it is at least as good as Phusion in terms of fidelity and coverage.%09%09%09%09%09%0A- In theory kapa hifi has higher fidelity than phusion.%09%09%09%09%09,700,Kapa HiFi test,,,hifi test,2, +3789280,Plasmodium Falciparum,,TTAGGCAT,,Standard,TTAGGCAT,,ems@sanger.ac.uk mq1@sanger.ac.uk,,ems@sanger.ac.uk mq1@sanger.ac.uk,mq1@sanger.ac.uk,,0,0,,,3D7200shear 3789280,Plasmodium Falciparum,5820,S0696,678,MQ R and D,standard,,,from:200 to:700,,,Plasmodium Falciparum,,,genomic DNA,,1289834,,3D7200shear,Plasmodium Falciparum,Plasmodium_falciparum (3D7),,,,1,0,0,- I have agreed to alpha test the kapa hifi qPCR kit. This allows prep PCR to be followed in real time and stopped when sufficient product has accumulated so preventing overcycling and allowing user to interrogate prep PCR step. %09%09%09%09%09%0A- If we are to use this enzyme we need to know that it is at least as good as Phusion in terms of fidelity and coverage.%09%09%09%09%09%0A- In theory kapa hifi has higher fidelity than phusion.%09%09%09%09%09,700,Kapa HiFi test,,,hifi test,3, +3789281,Homo sapiens,,TGACCACT,,Standard,TGACCACT,,ems@sanger.ac.uk mq1@sanger.ac.uk,,ems@sanger.ac.uk mq1@sanger.ac.uk,mq1@sanger.ac.uk,,0,0,,,human200shear 3789281,Homo sapiens,9606,S0696,678,MQ R and D,standard,,,from:200 to:700,,,Human,,,genomic DNA,,1289835,,human200shear,Homo sapiens,Homo_sapiens (GRCh37_53),,,,1,0,0,- I have agreed to alpha test the kapa hifi qPCR kit. This allows prep PCR to be followed in real time and stopped when sufficient product has accumulated so preventing overcycling and allowing user to interrogate prep PCR step. %09%09%09%09%09%0A- If we are to use this enzyme we need to know that it is at least as good as Phusion in terms of fidelity and coverage.%09%09%09%09%09%0A- In theory kapa hifi has higher fidelity than phusion.%09%09%09%09%09,700,Kapa HiFi test,,,hifi test,4, +3789282,Salmonella pullorum,,ACAGTGGT,,Standard,ACAGTGGT,,ems@sanger.ac.uk mq1@sanger.ac.uk,,ems@sanger.ac.uk mq1@sanger.ac.uk,mq1@sanger.ac.uk,,0,0,,,sp300shear 3789282,Salmonella pullorum,590,S0696,678,MQ R and D,standard,,,from:200 to:700,,,Salmonella pullorum,,,genomic DNA,,1289836,,sp300shear,Salmonella pullorum,Salmonella_pullorum (449_87),,,,1,0,0,- I have agreed to alpha test the kapa hifi qPCR kit. This allows prep PCR to be followed in real time and stopped when sufficient product has accumulated so preventing overcycling and allowing user to interrogate prep PCR step. %09%09%09%09%09%0A- If we are to use this enzyme we need to know that it is at least as good as Phusion in terms of fidelity and coverage.%09%09%09%09%09%0A- In theory kapa hifi has higher fidelity than phusion.%09%09%09%09%09,700,Kapa HiFi test,,,hifi test,5, +3789283,Bordetella Pertussis,,GCCAATGT,,Standard,GCCAATGT,,ems@sanger.ac.uk mq1@sanger.ac.uk,,ems@sanger.ac.uk mq1@sanger.ac.uk,mq1@sanger.ac.uk,,0,0,,,bp300shear 3789283,Bordetella Pertussis,520,S0696,678,MQ R and D,standard,,,from:200 to:700,,,Bordetella Pertussis,,,genomic DNA,,1289837,,bp300shear,Bordetella Pertussis,Bordetella_pertussis (ST24),,,,1,0,0,- I have agreed to alpha test the kapa hifi qPCR kit. This allows prep PCR to be followed in real time and stopped when sufficient product has accumulated so preventing overcycling and allowing user to interrogate prep PCR step. %09%09%09%09%09%0A- If we are to use this enzyme we need to know that it is at least as good as Phusion in terms of fidelity and coverage.%09%09%09%09%09%0A- In theory kapa hifi has higher fidelity than phusion.%09%09%09%09%09,700,Kapa HiFi test,,,hifi test,6, +3789284,Plasmodium Falciparum,,CAGATCTG,,Standard,CAGATCTG,,ems@sanger.ac.uk mq1@sanger.ac.uk,,ems@sanger.ac.uk mq1@sanger.ac.uk,mq1@sanger.ac.uk,,0,0,,,3D7300shear 3789284,Plasmodium Falciparum,5820,S0696,678,MQ R and D,standard,,,from:200 to:700,,,Plasmodium Falciparum,,,genomic DNA,,1289838,,3D7300shear,Plasmodium Falciparum,Plasmodium_falciparum (3D7),,,,1,0,0,- I have agreed to alpha test the kapa hifi qPCR kit. This allows prep PCR to be followed in real time and stopped when sufficient product has accumulated so preventing overcycling and allowing user to interrogate prep PCR step. %09%09%09%09%09%0A- If we are to use this enzyme we need to know that it is at least as good as Phusion in terms of fidelity and coverage.%09%09%09%09%09%0A- In theory kapa hifi has higher fidelity than phusion.%09%09%09%09%09,700,Kapa HiFi test,,,hifi test,7, +3789285,Homo sapiens,,ACTTGATG,,Standard,ACTTGATG,,ems@sanger.ac.uk mq1@sanger.ac.uk,,ems@sanger.ac.uk mq1@sanger.ac.uk,mq1@sanger.ac.uk,,0,0,,,human300shear 3789285,Homo sapiens,9606,S0696,678,MQ R and D,standard,,,from:200 to:700,,,Human,,,genomic DNA,,1289839,,human300shear,Homo sapiens,Homo_sapiens (GRCh37_53),,,,1,0,0,- I have agreed to alpha test the kapa hifi qPCR kit. This allows prep PCR to be followed in real time and stopped when sufficient product has accumulated so preventing overcycling and allowing user to interrogate prep PCR step. %09%09%09%09%09%0A- If we are to use this enzyme we need to know that it is at least as good as Phusion in terms of fidelity and coverage.%09%09%09%09%09%0A- In theory kapa hifi has higher fidelity than phusion.%09%09%09%09%09,700,Kapa HiFi test,,,hifi test,8, +3789286,Salmonella pullorum,,GATCAGCG,,Standard,GATCAGCG,,ems@sanger.ac.uk mq1@sanger.ac.uk,,ems@sanger.ac.uk mq1@sanger.ac.uk,mq1@sanger.ac.uk,,0,0,,,sp400shear 3789286,Salmonella pullorum,590,S0696,678,MQ R and D,standard,,,from:200 to:700,,,Salmonella pullorum,,,genomic DNA,,1289840,,sp400shear,Salmonella pullorum,Salmonella_pullorum (449_87),,,,1,0,0,- I have agreed to alpha test the kapa hifi qPCR kit. This allows prep PCR to be followed in real time and stopped when sufficient product has accumulated so preventing overcycling and allowing user to interrogate prep PCR step. %09%09%09%09%09%0A- If we are to use this enzyme we need to know that it is at least as good as Phusion in terms of fidelity and coverage.%09%09%09%09%09%0A- In theory kapa hifi has higher fidelity than phusion.%09%09%09%09%09,700,Kapa HiFi test,,,hifi test,9, +3789287,Bordetella Pertussis,,TAGCTTGT,,Standard,TAGCTTGT,,ems@sanger.ac.uk mq1@sanger.ac.uk,,ems@sanger.ac.uk mq1@sanger.ac.uk,mq1@sanger.ac.uk,,0,0,,,bp400shear 3789287,Bordetella Pertussis,520,S0696,678,MQ R and D,standard,,,from:200 to:700,,,Bordetella Pertussis,,,genomic DNA,,1289841,,bp400shear,Bordetella Pertussis,Bordetella_pertussis (ST24),,,,1,0,0,- I have agreed to alpha test the kapa hifi qPCR kit. This allows prep PCR to be followed in real time and stopped when sufficient product has accumulated so preventing overcycling and allowing user to interrogate prep PCR step. %09%09%09%09%09%0A- If we are to use this enzyme we need to know that it is at least as good as Phusion in terms of fidelity and coverage.%09%09%09%09%09%0A- In theory kapa hifi has higher fidelity than phusion.%09%09%09%09%09,700,Kapa HiFi test,,,hifi test,10, +3789288,Plasmodium Falciparum,,GGCTACAG,,Standard,GGCTACAG,,ems@sanger.ac.uk mq1@sanger.ac.uk,,ems@sanger.ac.uk mq1@sanger.ac.uk,mq1@sanger.ac.uk,,0,0,,,3D7400shear 3789288,Plasmodium Falciparum,5820,S0696,678,MQ R and D,standard,,,from:200 to:700,,,Plasmodium Falciparum,,,genomic DNA,,1289842,,3D7400shear,Plasmodium Falciparum,Plasmodium_falciparum (3D7),,,,1,0,0,- I have agreed to alpha test the kapa hifi qPCR kit. This allows prep PCR to be followed in real time and stopped when sufficient product has accumulated so preventing overcycling and allowing user to interrogate prep PCR step. %09%09%09%09%09%0A- If we are to use this enzyme we need to know that it is at least as good as Phusion in terms of fidelity and coverage.%09%09%09%09%09%0A- In theory kapa hifi has higher fidelity than phusion.%09%09%09%09%09,700,Kapa HiFi test,,,hifi test,11, +3789289,Homo sapiens,,CTTGTACT,,Standard,CTTGTACT,,ems@sanger.ac.uk mq1@sanger.ac.uk,,ems@sanger.ac.uk mq1@sanger.ac.uk,mq1@sanger.ac.uk,,0,0,,,human400shear 3789289,Homo sapiens,9606,S0696,678,MQ R and D,standard,,,from:200 to:700,,,Human,,,genomic DNA,,1289843,,human400shear,Homo sapiens,Homo_sapiens (GRCh37_53),,,,1,0,0,- I have agreed to alpha test the kapa hifi qPCR kit. This allows prep PCR to be followed in real time and stopped when sufficient product has accumulated so preventing overcycling and allowing user to interrogate prep PCR step. %09%09%09%09%09%0A- If we are to use this enzyme we need to know that it is at least as good as Phusion in terms of fidelity and coverage.%09%09%09%09%09%0A- In theory kapa hifi has higher fidelity than phusion.%09%09%09%09%09,700,Kapa HiFi test,,,hifi test,12, diff --git a/t/data/samplesheet/7007_extended.csv b/t/data/samplesheet/7007_extended.csv index 0feb664f..00208b36 100644 --- a/t/data/samplesheet/7007_extended.csv +++ b/t/data/samplesheet/7007_extended.csv @@ -1,3 +1,3 @@ -[Data],,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, -Sample_ID,Sample_Name,GenomeFolder,bait_name,default_library_type,default_tag_sequence,default_tagtwo_sequence,email_addresses,email_addresses_of_followers,email_addresses_of_managers,email_addresses_of_owners,gbs_plex_name,is_control,is_pool,lane_id,lane_priority,library_name,organism,organism_taxon_id,project_cost_code,project_id,project_name,purpose,qc_state,request_id,required_insert_size_range,sample_accession_number,sample_cohort,sample_common_name,sample_consent_withdrawn,sample_description,sample_donor_id,sample_id,sample_name,sample_public_name,sample_reference_genome,sample_supplier_name,spiked_phix_tag_index,study_accession_number,study_alignments_in_bam,study_contains_nonconsented_human,study_contains_nonconsented_xahuman,study_description,study_id,study_name,study_reference_genome,study_separate_y_chromosome_data,study_title,tag_index, -3789277,Strongyloides ratti,,,qPCR only,,,dg8@sanger.ac.uk neh@sanger.ac.uk,,dg8@sanger.ac.uk neh@sanger.ac.uk,neh@sanger.ac.uk,,0,0,3911772,0,SriL3_66K_212889 3789277,Strongyloides ratti,34506,S0702,521,Strongyloides ratti transcriptome,standard,pass,3642560,from:300 to:400,,,Strongyloides ratti,,,,1289830,SriL3_66K_212889,Strongyloides ratti,Strongyloides_ratti (20100601),,,,1,0,0,Strongyloides ratti is a common gastro-intestinal parasite of the rat. The adult parasites are female only%2C about 2mm long and live in the mucosa of the small intestine. These parasites produce eggs that pass out of the host in its faeces. In the environment infective larval stages develop either directly or after a facultative sexual free-living adult generation. Infective larvae infect hosts by skin penetration.%0A%0AS. ratti is the laboratory analogue of the parasite of humans%2C S. stercoralis. S. stercoralis is a wide-spread parasite of humans%2C occurring principally in the tropics and sub-tropics%3A some 100-200 million people are infected worldwide. Infection of immunosuppressed individuals can result in disseminated strongyloidiasis%2C in which worms occur throughout the body. This can be fatal unless anti-Strongyloides therapy is given. Other species of Strongyloides parasitise a wide range of vertebrates. %0A%0AAs part of the Strongyloides ratti genome project we are profiling the transcriptome of the parasite across its life cycle using RNA-Seq.. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria)%2C please see http%3A%2F%2Fwww.sanger.ac.uk%2Fdatasharing%2F,521,Strongyloides ratti transcriptomics,,,Transcriptome sequencing of Strongyloides ratti,, +[Data],,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +Sample_ID,Sample_Name,GenomeFolder,bait_name,default_library_type,default_tag_sequence,default_tagtwo_sequence,email_addresses,email_addresses_of_followers,email_addresses_of_managers,email_addresses_of_owners,gbs_plex_name,is_control,is_pool,lane_id,lane_priority,library_name,organism,organism_taxon_id,project_cost_code,project_id,project_name,purpose,qc_state,request_id,required_insert_size_range,sample_accession_number,sample_cohort,sample_common_name,sample_consent_withdrawn,sample_control_type,sample_description,sample_donor_id,sample_id,sample_is_control,sample_name,sample_public_name,sample_reference_genome,sample_supplier_name,spiked_phix_tag_index,study_accession_number,study_alignments_in_bam,study_contains_nonconsented_human,study_contains_nonconsented_xahuman,study_description,study_id,study_name,study_reference_genome,study_separate_y_chromosome_data,study_title,tag_index, +3789277,Strongyloides ratti,,,qPCR only,,,dg8@sanger.ac.uk neh@sanger.ac.uk,,dg8@sanger.ac.uk neh@sanger.ac.uk,neh@sanger.ac.uk,,0,0,3911772,0,SriL3_66K_212889 3789277,Strongyloides ratti,34506,S0702,521,Strongyloides ratti transcriptome,standard,pass,3642560,from:300 to:400,,,Strongyloides ratti,,,,,1289830,,SriL3_66K_212889,Strongyloides ratti,Strongyloides_ratti (20100601),,,,1,0,0,Strongyloides ratti is a common gastro-intestinal parasite of the rat. The adult parasites are female only%2C about 2mm long and live in the mucosa of the small intestine. These parasites produce eggs that pass out of the host in its faeces. In the environment infective larval stages develop either directly or after a facultative sexual free-living adult generation. Infective larvae infect hosts by skin penetration.%0A%0AS. ratti is the laboratory analogue of the parasite of humans%2C S. stercoralis. S. stercoralis is a wide-spread parasite of humans%2C occurring principally in the tropics and sub-tropics%3A some 100-200 million people are infected worldwide. Infection of immunosuppressed individuals can result in disseminated strongyloidiasis%2C in which worms occur throughout the body. This can be fatal unless anti-Strongyloides therapy is given. Other species of Strongyloides parasitise a wide range of vertebrates. %0A%0AAs part of the Strongyloides ratti genome project we are profiling the transcriptome of the parasite across its life cycle using RNA-Seq.. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria)%2C please see http%3A%2F%2Fwww.sanger.ac.uk%2Fdatasharing%2F,521,Strongyloides ratti transcriptomics,,,Transcriptome sequencing of Strongyloides ratti,, diff --git a/t/data/samplesheet/8pools_extended.csv b/t/data/samplesheet/8pools_extended.csv index f68abb9d..509aeff5 100644 --- a/t/data/samplesheet/8pools_extended.csv +++ b/t/data/samplesheet/8pools_extended.csv @@ -1,70 +1,70 @@ -[Data],,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, -Lane,Sample_ID,Sample_Name,GenomeFolder,Index,bait_name,default_library_type,default_tag_sequence,default_tagtwo_sequence,email_addresses,email_addresses_of_followers,email_addresses_of_managers,email_addresses_of_owners,gbs_plex_name,is_control,is_pool,lane_id,lane_priority,library_name,organism,organism_taxon_id,project_cost_code,project_id,project_name,purpose,qc_state,request_id,required_insert_size_range,sample_accession_number,sample_cohort,sample_common_name,sample_consent_withdrawn,sample_description,sample_donor_id,sample_id,sample_name,sample_public_name,sample_reference_genome,sample_supplier_name,spiked_phix_tag_index,study_accession_number,study_alignments_in_bam,study_contains_nonconsented_human,study_contains_nonconsented_xahuman,study_description,study_id,study_name,study_reference_genome,study_separate_y_chromosome_data,study_title,tag_index, -1,4488737,EGAN00001017479,,ATCACG,Human all exon 50MB,Agilent Pulldown,ATCACG,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017479,,Homo sapiens,,,,1092801,UK10K_UKSCZ5072921,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,1, -1,4488725,EGAN00001017480,,CGATGT,Human all exon 50MB,Agilent Pulldown,CGATGT,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017480,,Homo sapiens,,,,1092803,UK10K_UKSCZ5072922,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,2, -1,4488713,EGAN00001017481,,TTAGGC,Human all exon 50MB,Agilent Pulldown,TTAGGC,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017481,,Homo sapiens,,,,1092805,UK10K_UKSCZ5072923,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,3, -1,4488701,EGAN00001017482,,TGACCA,Human all exon 50MB,Agilent Pulldown,TGACCA,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017482,,Homo sapiens,,,,1092807,UK10K_UKSCZ5072924,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,4, -1,4488689,EGAN00001017483,,ACAGTG,Human all exon 50MB,Agilent Pulldown,ACAGTG,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017483,,Homo sapiens,,,,1092809,UK10K_UKSCZ5072925,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,5, -1,4488677,EGAN00001017484,,GCCAAT,Human all exon 50MB,Agilent Pulldown,GCCAAT,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017484,,Homo sapiens,,,,1092811,UK10K_UKSCZ5072926,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,6, -1,4488665,EGAN00001017485,,CAGATC,Human all exon 50MB,Agilent Pulldown,CAGATC,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017485,,Homo sapiens,,,,1092813,UK10K_UKSCZ5072927,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,7, -1,4488653,EGAN00001017478,,ACTTGA,Human all exon 50MB,Agilent Pulldown,ACTTGA,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017478,,Homo sapiens,,,,1092799,UK10K_UKSCZ5072920,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,8, -1,4405872,phiX_for_spiked_buffers,,ACAACGCAAT,,,ACAACGCAAT,,hps@sanger.ac.uk,,hps@sanger.ac.uk,hps@sanger.ac.uk,,1,0,,,PhiX (10Jan12),,,,,,standard,,,,,,,,,,1255141,phiX_for_spiked_buffers,,,,168,,1,0,0,None,198,Illumina Controls,,,,168, -2,4488736,EGAN00001017487,,ATCACG,Human all exon 50MB,Agilent Pulldown,ATCACG,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017487,,Homo sapiens,,,,1092817,UK10K_UKSCZ5072929,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,1, -2,4488724,EGAN00001017488,,CGATGT,Human all exon 50MB,Agilent Pulldown,CGATGT,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017488,,Homo sapiens,,,,1092819,UK10K_UKSCZ5072930,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,2, -2,4488712,EGAN00001017489,,TTAGGC,Human all exon 50MB,Agilent Pulldown,TTAGGC,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017489,,Homo sapiens,,,,1092821,UK10K_UKSCZ5072931,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,3, -2,4488700,EGAN00001017490,,TGACCA,Human all exon 50MB,Agilent Pulldown,TGACCA,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017490,,Homo sapiens,,,,1092823,UK10K_UKSCZ5072932,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,4, -2,4488688,EGAN00001017491,,ACAGTG,Human all exon 50MB,Agilent Pulldown,ACAGTG,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017491,,Homo sapiens,,,,1092825,UK10K_UKSCZ5072933,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,5, -2,4488676,EGAN00001017486,,GCCAAT,Human all exon 50MB,Agilent Pulldown,GCCAAT,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017486,,Homo sapiens,,,,1092815,UK10K_UKSCZ5072928,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,6, -2,4405872,phiX_for_spiked_buffers,,ACAACGCAAT,,,ACAACGCAAT,,hps@sanger.ac.uk,,hps@sanger.ac.uk,hps@sanger.ac.uk,,1,0,,,PhiX (10Jan12),,,,,,standard,,,,,,,,,,1255141,phiX_for_spiked_buffers,,,,168,,1,0,0,None,198,Illumina Controls,,,,168, -3,4488940,EGAN00001017496,,ATCACG,Human all exon 50MB,Agilent Pulldown,ATCACG,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017496,,Homo sapiens,,,,1092835,UK10K_UKSCZ5072938,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,1, -3,4488928,EGAN00001017497,,CGATGT,Human all exon 50MB,Agilent Pulldown,CGATGT,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017497,,Homo sapiens,,,,1092837,UK10K_UKSCZ5072939,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,2, -3,4488916,EGAN00001017498,,TTAGGC,Human all exon 50MB,Agilent Pulldown,TTAGGC,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017498,,Homo sapiens,,,,1092839,UK10K_UKSCZ5072940,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,3, -3,4488904,EGAN00001017499,,TGACCA,Human all exon 50MB,Agilent Pulldown,TGACCA,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017499,,Homo sapiens,,,,1092841,UK10K_UKSCZ5072941,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,4, -3,4488892,EGAN00001017492,,ACAGTG,Human all exon 50MB,Agilent Pulldown,ACAGTG,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017492,,Homo sapiens,,,,1092827,UK10K_UKSCZ5072934,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,5, -3,4488880,EGAN00001017493,,GCCAAT,Human all exon 50MB,Agilent Pulldown,GCCAAT,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017493,,Homo sapiens,,,,1092829,UK10K_UKSCZ5072935,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,6, -3,4488868,EGAN00001017494,,CAGATC,Human all exon 50MB,Agilent Pulldown,CAGATC,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017494,,Homo sapiens,,,,1092831,UK10K_UKSCZ5072936,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,7, -3,4488857,EGAN00001017495,,ACTTGA,Human all exon 50MB,Agilent Pulldown,ACTTGA,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017495,,Homo sapiens,,,,1092833,UK10K_UKSCZ5072937,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,8, -3,4405872,phiX_for_spiked_buffers,,ACAACGCAAT,,,ACAACGCAAT,,hps@sanger.ac.uk,,hps@sanger.ac.uk,hps@sanger.ac.uk,,1,0,,,PhiX (10Jan12),,,,,,standard,,,,,,,,,,1255141,phiX_for_spiked_buffers,,,,168,,1,0,0,None,198,Illumina Controls,,,,168, -4,4488939,EGAN00001017504,,ATCACG,Human all exon 50MB,Agilent Pulldown,ATCACG,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017504,,Homo sapiens,,,,1092851,UK10K_UKSCZ5072946,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,1, -4,4488927,EGAN00001017505,,CGATGT,Human all exon 50MB,Agilent Pulldown,CGATGT,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017505,,Homo sapiens,,,,1092853,UK10K_UKSCZ5072947,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,2, -4,4488915,EGAN00001017506,,TTAGGC,Human all exon 50MB,Agilent Pulldown,TTAGGC,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017506,,Homo sapiens,,,,1092855,UK10K_UKSCZ5072948,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,3, -4,4488903,EGAN00001017507,,TGACCA,Human all exon 50MB,Agilent Pulldown,TGACCA,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017507,,Homo sapiens,,,,1092857,UK10K_UKSCZ5072949,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,4, -4,4488891,EGAN00001017500,,ACAGTG,Human all exon 50MB,Agilent Pulldown,ACAGTG,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017500,,Homo sapiens,,,,1092843,UK10K_UKSCZ5072942,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,5, -4,4488879,EGAN00001017501,,GCCAAT,Human all exon 50MB,Agilent Pulldown,GCCAAT,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017501,,Homo sapiens,,,,1092845,UK10K_UKSCZ5072943,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,6, -4,4488867,EGAN00001017502,,CAGATC,Human all exon 50MB,Agilent Pulldown,CAGATC,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017502,,Homo sapiens,,,,1092847,UK10K_UKSCZ5072944,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,7, -4,4488856,EGAN00001017503,,ACTTGA,Human all exon 50MB,Agilent Pulldown,ACTTGA,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017503,,Homo sapiens,,,,1092849,UK10K_UKSCZ5072945,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,8, -4,4405872,phiX_for_spiked_buffers,,ACAACGCAAT,,,ACAACGCAAT,,hps@sanger.ac.uk,,hps@sanger.ac.uk,hps@sanger.ac.uk,,1,0,,,PhiX (10Jan12),,,,,,standard,,,,,,,,,,1255141,phiX_for_spiked_buffers,,,,168,,1,0,0,None,198,Illumina Controls,,,,168, -5,4488938,EGAN00001017512,,ATCACG,Human all exon 50MB,Agilent Pulldown,ATCACG,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017512,,Homo sapiens,,,,1092867,UK10K_UKSCZ5072954,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,1, -5,4488926,EGAN00001017513,,CGATGT,Human all exon 50MB,Agilent Pulldown,CGATGT,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017513,,Homo sapiens,,,,1092869,UK10K_UKSCZ5072955,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,2, -5,4488914,EGAN00001083713,,TTAGGC,Human all exon 50MB,Agilent Pulldown,TTAGGC,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083713,,Homo sapiens,,,,1318722,UK10K_UKSCZ5260646,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,3, -5,4488902,EGAN00001083715,,TGACCA,Human all exon 50MB,Agilent Pulldown,TGACCA,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083715,,Homo sapiens,,,,1318724,UK10K_UKSCZ5260647,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,4, -5,4488890,EGAN00001017508,,ACAGTG,Human all exon 50MB,Agilent Pulldown,ACAGTG,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017508,,Homo sapiens,,,,1092859,UK10K_UKSCZ5072950,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,5, -5,4488878,EGAN00001017509,,GCCAAT,Human all exon 50MB,Agilent Pulldown,GCCAAT,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017509,,Homo sapiens,,,,1092861,UK10K_UKSCZ5072951,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,6, -5,4488866,EGAN00001017510,,CAGATC,Human all exon 50MB,Agilent Pulldown,CAGATC,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017510,,Homo sapiens,,,,1092863,UK10K_UKSCZ5072952,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,7, -5,4488855,EGAN00001017511,,ACTTGA,Human all exon 50MB,Agilent Pulldown,ACTTGA,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017511,,Homo sapiens,,,,1092865,UK10K_UKSCZ5072953,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,8, -5,4405872,phiX_for_spiked_buffers,,ACAACGCAAT,,,ACAACGCAAT,,hps@sanger.ac.uk,,hps@sanger.ac.uk,hps@sanger.ac.uk,,1,0,,,PhiX (10Jan12),,,,,,standard,,,,,,,,,,1255141,phiX_for_spiked_buffers,,,,168,,1,0,0,None,198,Illumina Controls,,,,168, -6,4488937,EGAN00001083723,,ATCACG,Human all exon 50MB,Agilent Pulldown,ATCACG,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083723,,Homo sapiens,,,,1318732,UK10K_UKSCZ5260651,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,1, -6,4488925,EGAN00001083725,,CGATGT,Human all exon 50MB,Agilent Pulldown,CGATGT,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083725,,Homo sapiens,,,,1318734,UK10K_UKSCZ5260652,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,2, -6,4488913,EGAN00001083728,,TTAGGC,Human all exon 50MB,Agilent Pulldown,TTAGGC,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083728,,Homo sapiens,,,,1318737,UK10K_UKSCZ5260653,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,3, -6,4488901,EGAN00001083729,,TGACCA,Human all exon 50MB,Agilent Pulldown,TGACCA,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083729,,Homo sapiens,,,,1318738,UK10K_UKSCZ5260654,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,4, -6,4488877,EGAN00001083717,,GCCAAT,Human all exon 50MB,Agilent Pulldown,GCCAAT,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083717,,Homo sapiens,,,,1318726,UK10K_UKSCZ5260648,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,6, -6,4488865,EGAN00001083719,,CAGATC,Human all exon 50MB,Agilent Pulldown,CAGATC,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083719,,Homo sapiens,,,,1318728,UK10K_UKSCZ5260649,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,7, -6,4488854,EGAN00001083721,,ACTTGA,Human all exon 50MB,Agilent Pulldown,ACTTGA,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083721,,Homo sapiens,,,,1318730,UK10K_UKSCZ5260650,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,8, -6,4405872,phiX_for_spiked_buffers,,ACAACGCAAT,,,ACAACGCAAT,,hps@sanger.ac.uk,,hps@sanger.ac.uk,hps@sanger.ac.uk,,1,0,,,PhiX (10Jan12),,,,,,standard,,,,,,,,,,1255141,phiX_for_spiked_buffers,,,,168,,1,0,0,None,198,Illumina Controls,,,,168, -7,4488936,EGAN00001083740,,ATCACG,Human all exon 50MB,Agilent Pulldown,ATCACG,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083740,,Homo sapiens,,,,1318749,UK10K_UKSCZ5260659,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,1, -7,4488924,EGAN00001083742,,CGATGT,Human all exon 50MB,Agilent Pulldown,CGATGT,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083742,,Homo sapiens,,,,1318751,UK10K_UKSCZ5260660,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,2, -7,4488912,EGAN00001083744,,TTAGGC,Human all exon 50MB,Agilent Pulldown,TTAGGC,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083744,,Homo sapiens,,,,1318753,UK10K_UKSCZ5260661,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,3, -7,4488900,EGAN00001083747,,TGACCA,Human all exon 50MB,Agilent Pulldown,TGACCA,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083747,,Homo sapiens,,,,1318756,UK10K_UKSCZ5260662,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,4, -7,4488888,EGAN00001083748,,ACAGTG,Human all exon 50MB,Agilent Pulldown,ACAGTG,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083748,,Homo sapiens,,,,1318757,UK10K_UKSCZ5260663,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,5, -7,4488876,EGAN00001083734,,GCCAAT,Human all exon 50MB,Agilent Pulldown,GCCAAT,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083734,,Homo sapiens,,,,1318743,UK10K_UKSCZ5260656,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,6, -7,4488864,EGAN00001083736,,CAGATC,Human all exon 50MB,Agilent Pulldown,CAGATC,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083736,,Homo sapiens,,,,1318745,UK10K_UKSCZ5260657,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,7, -7,4488853,EGAN00001083738,,ACTTGA,Human all exon 50MB,Agilent Pulldown,ACTTGA,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083738,,Homo sapiens,,,,1318747,UK10K_UKSCZ5260658,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,8, -7,4405872,phiX_for_spiked_buffers,,ACAACGCAAT,,,ACAACGCAAT,,hps@sanger.ac.uk,,hps@sanger.ac.uk,hps@sanger.ac.uk,,1,0,,,PhiX (10Jan12),,,,,,standard,,,,,,,,,,1255141,phiX_for_spiked_buffers,,,,168,,1,0,0,None,198,Illumina Controls,,,,168, -8,4488929,EGAN00001083761,,CGATGT,Human all exon 50MB,Agilent Pulldown,CGATGT,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083761,,Homo sapiens,,,,1318770,UK10K_UKSCZ5260668,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,2, -8,4488917,EGAN00001083763,,TTAGGC,Human all exon 50MB,Agilent Pulldown,TTAGGC,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083763,,Homo sapiens,,,,1318772,UK10K_UKSCZ5260669,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,3, -8,4488905,EGAN00001083764,,TGACCA,Human all exon 50MB,Agilent Pulldown,TGACCA,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083764,,Homo sapiens,,,,1318773,UK10K_UKSCZ5260670,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,4, -8,4488893,EGAN00001083765,,ACAGTG,Human all exon 50MB,Agilent Pulldown,ACAGTG,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083765,,Homo sapiens,,,,1318774,UK10K_UKSCZ5260671,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,5, -8,4488881,EGAN00001083749,,GCCAAT,Human all exon 50MB,Agilent Pulldown,GCCAAT,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083749,,Homo sapiens,,,,1318758,UK10K_UKSCZ5260664,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,6, -8,4488869,EGAN00001083750,,CAGATC,Human all exon 50MB,Agilent Pulldown,CAGATC,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083750,,Homo sapiens,,,,1318759,UK10K_UKSCZ5260665,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,7, -8,4488858,EGAN00001083751,,ACTTGA,Human all exon 50MB,Agilent Pulldown,ACTTGA,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083751,,Homo sapiens,,,,1318760,UK10K_UKSCZ5260666,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,8, -8,4405872,phiX_for_spiked_buffers,,ACAACGCAAT,,,ACAACGCAAT,,hps@sanger.ac.uk,,hps@sanger.ac.uk,hps@sanger.ac.uk,,1,0,,,PhiX (10Jan12),,,,,,standard,,,,,,,,,,1255141,phiX_for_spiked_buffers,,,,168,,1,0,0,None,198,Illumina Controls,,,,168, +[Data],,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +Lane,Sample_ID,Sample_Name,GenomeFolder,Index,bait_name,default_library_type,default_tag_sequence,default_tagtwo_sequence,email_addresses,email_addresses_of_followers,email_addresses_of_managers,email_addresses_of_owners,gbs_plex_name,is_control,is_pool,lane_id,lane_priority,library_name,organism,organism_taxon_id,project_cost_code,project_id,project_name,purpose,qc_state,request_id,required_insert_size_range,sample_accession_number,sample_cohort,sample_common_name,sample_consent_withdrawn,sample_control_type,sample_description,sample_donor_id,sample_id,sample_is_control,sample_name,sample_public_name,sample_reference_genome,sample_supplier_name,spiked_phix_tag_index,study_accession_number,study_alignments_in_bam,study_contains_nonconsented_human,study_contains_nonconsented_xahuman,study_description,study_id,study_name,study_reference_genome,study_separate_y_chromosome_data,study_title,tag_index, +1,4488737,EGAN00001017479,,ATCACG,Human all exon 50MB,Agilent Pulldown,ATCACG,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017479,,Homo sapiens,,,,,1092801,,UK10K_UKSCZ5072921,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,1, +1,4488725,EGAN00001017480,,CGATGT,Human all exon 50MB,Agilent Pulldown,CGATGT,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017480,,Homo sapiens,,,,,1092803,,UK10K_UKSCZ5072922,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,2, +1,4488713,EGAN00001017481,,TTAGGC,Human all exon 50MB,Agilent Pulldown,TTAGGC,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017481,,Homo sapiens,,,,,1092805,,UK10K_UKSCZ5072923,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,3, +1,4488701,EGAN00001017482,,TGACCA,Human all exon 50MB,Agilent Pulldown,TGACCA,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017482,,Homo sapiens,,,,,1092807,,UK10K_UKSCZ5072924,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,4, +1,4488689,EGAN00001017483,,ACAGTG,Human all exon 50MB,Agilent Pulldown,ACAGTG,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017483,,Homo sapiens,,,,,1092809,,UK10K_UKSCZ5072925,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,5, +1,4488677,EGAN00001017484,,GCCAAT,Human all exon 50MB,Agilent Pulldown,GCCAAT,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017484,,Homo sapiens,,,,,1092811,,UK10K_UKSCZ5072926,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,6, +1,4488665,EGAN00001017485,,CAGATC,Human all exon 50MB,Agilent Pulldown,CAGATC,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017485,,Homo sapiens,,,,,1092813,,UK10K_UKSCZ5072927,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,7, +1,4488653,EGAN00001017478,,ACTTGA,Human all exon 50MB,Agilent Pulldown,ACTTGA,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017478,,Homo sapiens,,,,,1092799,,UK10K_UKSCZ5072920,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,8, +1,4405872,phiX_for_spiked_buffers,,ACAACGCAAT,,,ACAACGCAAT,,hps@sanger.ac.uk,,hps@sanger.ac.uk,hps@sanger.ac.uk,,1,0,,,PhiX (10Jan12),,,,,,standard,,,,,,,,,,,1255141,,phiX_for_spiked_buffers,,,,168,,1,0,0,None,198,Illumina Controls,,,,168, +2,4488736,EGAN00001017487,,ATCACG,Human all exon 50MB,Agilent Pulldown,ATCACG,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017487,,Homo sapiens,,,,,1092817,,UK10K_UKSCZ5072929,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,1, +2,4488724,EGAN00001017488,,CGATGT,Human all exon 50MB,Agilent Pulldown,CGATGT,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017488,,Homo sapiens,,,,,1092819,,UK10K_UKSCZ5072930,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,2, +2,4488712,EGAN00001017489,,TTAGGC,Human all exon 50MB,Agilent Pulldown,TTAGGC,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017489,,Homo sapiens,,,,,1092821,,UK10K_UKSCZ5072931,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,3, +2,4488700,EGAN00001017490,,TGACCA,Human all exon 50MB,Agilent Pulldown,TGACCA,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017490,,Homo sapiens,,,,,1092823,,UK10K_UKSCZ5072932,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,4, +2,4488688,EGAN00001017491,,ACAGTG,Human all exon 50MB,Agilent Pulldown,ACAGTG,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017491,,Homo sapiens,,,,,1092825,,UK10K_UKSCZ5072933,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,5, +2,4488676,EGAN00001017486,,GCCAAT,Human all exon 50MB,Agilent Pulldown,GCCAAT,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017486,,Homo sapiens,,,,,1092815,,UK10K_UKSCZ5072928,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,6, +2,4405872,phiX_for_spiked_buffers,,ACAACGCAAT,,,ACAACGCAAT,,hps@sanger.ac.uk,,hps@sanger.ac.uk,hps@sanger.ac.uk,,1,0,,,PhiX (10Jan12),,,,,,standard,,,,,,,,,,,1255141,,phiX_for_spiked_buffers,,,,168,,1,0,0,None,198,Illumina Controls,,,,168, +3,4488940,EGAN00001017496,,ATCACG,Human all exon 50MB,Agilent Pulldown,ATCACG,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017496,,Homo sapiens,,,,,1092835,,UK10K_UKSCZ5072938,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,1, +3,4488928,EGAN00001017497,,CGATGT,Human all exon 50MB,Agilent Pulldown,CGATGT,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017497,,Homo sapiens,,,,,1092837,,UK10K_UKSCZ5072939,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,2, +3,4488916,EGAN00001017498,,TTAGGC,Human all exon 50MB,Agilent Pulldown,TTAGGC,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017498,,Homo sapiens,,,,,1092839,,UK10K_UKSCZ5072940,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,3, +3,4488904,EGAN00001017499,,TGACCA,Human all exon 50MB,Agilent Pulldown,TGACCA,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017499,,Homo sapiens,,,,,1092841,,UK10K_UKSCZ5072941,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,4, +3,4488892,EGAN00001017492,,ACAGTG,Human all exon 50MB,Agilent Pulldown,ACAGTG,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017492,,Homo sapiens,,,,,1092827,,UK10K_UKSCZ5072934,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,5, +3,4488880,EGAN00001017493,,GCCAAT,Human all exon 50MB,Agilent Pulldown,GCCAAT,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017493,,Homo sapiens,,,,,1092829,,UK10K_UKSCZ5072935,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,6, +3,4488868,EGAN00001017494,,CAGATC,Human all exon 50MB,Agilent Pulldown,CAGATC,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017494,,Homo sapiens,,,,,1092831,,UK10K_UKSCZ5072936,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,7, +3,4488857,EGAN00001017495,,ACTTGA,Human all exon 50MB,Agilent Pulldown,ACTTGA,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017495,,Homo sapiens,,,,,1092833,,UK10K_UKSCZ5072937,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,8, +3,4405872,phiX_for_spiked_buffers,,ACAACGCAAT,,,ACAACGCAAT,,hps@sanger.ac.uk,,hps@sanger.ac.uk,hps@sanger.ac.uk,,1,0,,,PhiX (10Jan12),,,,,,standard,,,,,,,,,,,1255141,,phiX_for_spiked_buffers,,,,168,,1,0,0,None,198,Illumina Controls,,,,168, +4,4488939,EGAN00001017504,,ATCACG,Human all exon 50MB,Agilent Pulldown,ATCACG,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017504,,Homo sapiens,,,,,1092851,,UK10K_UKSCZ5072946,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,1, +4,4488927,EGAN00001017505,,CGATGT,Human all exon 50MB,Agilent Pulldown,CGATGT,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017505,,Homo sapiens,,,,,1092853,,UK10K_UKSCZ5072947,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,2, +4,4488915,EGAN00001017506,,TTAGGC,Human all exon 50MB,Agilent Pulldown,TTAGGC,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017506,,Homo sapiens,,,,,1092855,,UK10K_UKSCZ5072948,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,3, +4,4488903,EGAN00001017507,,TGACCA,Human all exon 50MB,Agilent Pulldown,TGACCA,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017507,,Homo sapiens,,,,,1092857,,UK10K_UKSCZ5072949,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,4, +4,4488891,EGAN00001017500,,ACAGTG,Human all exon 50MB,Agilent Pulldown,ACAGTG,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017500,,Homo sapiens,,,,,1092843,,UK10K_UKSCZ5072942,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,5, +4,4488879,EGAN00001017501,,GCCAAT,Human all exon 50MB,Agilent Pulldown,GCCAAT,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017501,,Homo sapiens,,,,,1092845,,UK10K_UKSCZ5072943,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,6, +4,4488867,EGAN00001017502,,CAGATC,Human all exon 50MB,Agilent Pulldown,CAGATC,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017502,,Homo sapiens,,,,,1092847,,UK10K_UKSCZ5072944,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,7, +4,4488856,EGAN00001017503,,ACTTGA,Human all exon 50MB,Agilent Pulldown,ACTTGA,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017503,,Homo sapiens,,,,,1092849,,UK10K_UKSCZ5072945,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,8, +4,4405872,phiX_for_spiked_buffers,,ACAACGCAAT,,,ACAACGCAAT,,hps@sanger.ac.uk,,hps@sanger.ac.uk,hps@sanger.ac.uk,,1,0,,,PhiX (10Jan12),,,,,,standard,,,,,,,,,,,1255141,,phiX_for_spiked_buffers,,,,168,,1,0,0,None,198,Illumina Controls,,,,168, +5,4488938,EGAN00001017512,,ATCACG,Human all exon 50MB,Agilent Pulldown,ATCACG,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017512,,Homo sapiens,,,,,1092867,,UK10K_UKSCZ5072954,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,1, +5,4488926,EGAN00001017513,,CGATGT,Human all exon 50MB,Agilent Pulldown,CGATGT,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017513,,Homo sapiens,,,,,1092869,,UK10K_UKSCZ5072955,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,2, +5,4488914,EGAN00001083713,,TTAGGC,Human all exon 50MB,Agilent Pulldown,TTAGGC,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083713,,Homo sapiens,,,,,1318722,,UK10K_UKSCZ5260646,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,3, +5,4488902,EGAN00001083715,,TGACCA,Human all exon 50MB,Agilent Pulldown,TGACCA,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083715,,Homo sapiens,,,,,1318724,,UK10K_UKSCZ5260647,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,4, +5,4488890,EGAN00001017508,,ACAGTG,Human all exon 50MB,Agilent Pulldown,ACAGTG,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017508,,Homo sapiens,,,,,1092859,,UK10K_UKSCZ5072950,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,5, +5,4488878,EGAN00001017509,,GCCAAT,Human all exon 50MB,Agilent Pulldown,GCCAAT,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017509,,Homo sapiens,,,,,1092861,,UK10K_UKSCZ5072951,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,6, +5,4488866,EGAN00001017510,,CAGATC,Human all exon 50MB,Agilent Pulldown,CAGATC,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017510,,Homo sapiens,,,,,1092863,,UK10K_UKSCZ5072952,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,7, +5,4488855,EGAN00001017511,,ACTTGA,Human all exon 50MB,Agilent Pulldown,ACTTGA,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001017511,,Homo sapiens,,,,,1092865,,UK10K_UKSCZ5072953,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,8, +5,4405872,phiX_for_spiked_buffers,,ACAACGCAAT,,,ACAACGCAAT,,hps@sanger.ac.uk,,hps@sanger.ac.uk,hps@sanger.ac.uk,,1,0,,,PhiX (10Jan12),,,,,,standard,,,,,,,,,,,1255141,,phiX_for_spiked_buffers,,,,168,,1,0,0,None,198,Illumina Controls,,,,168, +6,4488937,EGAN00001083723,,ATCACG,Human all exon 50MB,Agilent Pulldown,ATCACG,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083723,,Homo sapiens,,,,,1318732,,UK10K_UKSCZ5260651,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,1, +6,4488925,EGAN00001083725,,CGATGT,Human all exon 50MB,Agilent Pulldown,CGATGT,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083725,,Homo sapiens,,,,,1318734,,UK10K_UKSCZ5260652,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,2, +6,4488913,EGAN00001083728,,TTAGGC,Human all exon 50MB,Agilent Pulldown,TTAGGC,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083728,,Homo sapiens,,,,,1318737,,UK10K_UKSCZ5260653,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,3, +6,4488901,EGAN00001083729,,TGACCA,Human all exon 50MB,Agilent Pulldown,TGACCA,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083729,,Homo sapiens,,,,,1318738,,UK10K_UKSCZ5260654,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,4, +6,4488877,EGAN00001083717,,GCCAAT,Human all exon 50MB,Agilent Pulldown,GCCAAT,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083717,,Homo sapiens,,,,,1318726,,UK10K_UKSCZ5260648,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,6, +6,4488865,EGAN00001083719,,CAGATC,Human all exon 50MB,Agilent Pulldown,CAGATC,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083719,,Homo sapiens,,,,,1318728,,UK10K_UKSCZ5260649,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,7, +6,4488854,EGAN00001083721,,ACTTGA,Human all exon 50MB,Agilent Pulldown,ACTTGA,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083721,,Homo sapiens,,,,,1318730,,UK10K_UKSCZ5260650,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,8, +6,4405872,phiX_for_spiked_buffers,,ACAACGCAAT,,,ACAACGCAAT,,hps@sanger.ac.uk,,hps@sanger.ac.uk,hps@sanger.ac.uk,,1,0,,,PhiX (10Jan12),,,,,,standard,,,,,,,,,,,1255141,,phiX_for_spiked_buffers,,,,168,,1,0,0,None,198,Illumina Controls,,,,168, +7,4488936,EGAN00001083740,,ATCACG,Human all exon 50MB,Agilent Pulldown,ATCACG,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083740,,Homo sapiens,,,,,1318749,,UK10K_UKSCZ5260659,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,1, +7,4488924,EGAN00001083742,,CGATGT,Human all exon 50MB,Agilent Pulldown,CGATGT,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083742,,Homo sapiens,,,,,1318751,,UK10K_UKSCZ5260660,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,2, +7,4488912,EGAN00001083744,,TTAGGC,Human all exon 50MB,Agilent Pulldown,TTAGGC,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083744,,Homo sapiens,,,,,1318753,,UK10K_UKSCZ5260661,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,3, +7,4488900,EGAN00001083747,,TGACCA,Human all exon 50MB,Agilent Pulldown,TGACCA,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083747,,Homo sapiens,,,,,1318756,,UK10K_UKSCZ5260662,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,4, +7,4488888,EGAN00001083748,,ACAGTG,Human all exon 50MB,Agilent Pulldown,ACAGTG,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083748,,Homo sapiens,,,,,1318757,,UK10K_UKSCZ5260663,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,5, +7,4488876,EGAN00001083734,,GCCAAT,Human all exon 50MB,Agilent Pulldown,GCCAAT,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083734,,Homo sapiens,,,,,1318743,,UK10K_UKSCZ5260656,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,6, +7,4488864,EGAN00001083736,,CAGATC,Human all exon 50MB,Agilent Pulldown,CAGATC,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083736,,Homo sapiens,,,,,1318745,,UK10K_UKSCZ5260657,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,7, +7,4488853,EGAN00001083738,,ACTTGA,Human all exon 50MB,Agilent Pulldown,ACTTGA,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083738,,Homo sapiens,,,,,1318747,,UK10K_UKSCZ5260658,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,8, +7,4405872,phiX_for_spiked_buffers,,ACAACGCAAT,,,ACAACGCAAT,,hps@sanger.ac.uk,,hps@sanger.ac.uk,hps@sanger.ac.uk,,1,0,,,PhiX (10Jan12),,,,,,standard,,,,,,,,,,,1255141,,phiX_for_spiked_buffers,,,,168,,1,0,0,None,198,Illumina Controls,,,,168, +8,4488929,EGAN00001083761,,CGATGT,Human all exon 50MB,Agilent Pulldown,CGATGT,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083761,,Homo sapiens,,,,,1318770,,UK10K_UKSCZ5260668,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,2, +8,4488917,EGAN00001083763,,TTAGGC,Human all exon 50MB,Agilent Pulldown,TTAGGC,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083763,,Homo sapiens,,,,,1318772,,UK10K_UKSCZ5260669,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,3, +8,4488905,EGAN00001083764,,TGACCA,Human all exon 50MB,Agilent Pulldown,TGACCA,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083764,,Homo sapiens,,,,,1318773,,UK10K_UKSCZ5260670,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,4, +8,4488893,EGAN00001083765,,ACAGTG,Human all exon 50MB,Agilent Pulldown,ACAGTG,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083765,,Homo sapiens,,,,,1318774,,UK10K_UKSCZ5260671,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,5, +8,4488881,EGAN00001083749,,GCCAAT,Human all exon 50MB,Agilent Pulldown,GCCAAT,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083749,,Homo sapiens,,,,,1318758,,UK10K_UKSCZ5260664,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,6, +8,4488869,EGAN00001083750,,CAGATC,Human all exon 50MB,Agilent Pulldown,CAGATC,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083750,,Homo sapiens,,,,,1318759,,UK10K_UKSCZ5260665,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,7, +8,4488858,EGAN00001083751,,ACTTGA,Human all exon 50MB,Agilent Pulldown,ACTTGA,,cj5@sanger.ac.uk elg@sanger.ac.uk jws@sanger.ac.uk sm15@sanger.ac.uk,cj5@sanger.ac.uk elg@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,jws@sanger.ac.uk sm15@sanger.ac.uk,,0,0,,,,,9606,S0782,645,UK10K,standard,,,from:100 to:400,EGAN00001083751,,Homo sapiens,,,,,1318760,,UK10K_UKSCZ5260666,,,,168,EGAS00001000123,1,0,0,%09In the UK10K project we propose a series of complementary genetic approaches to find new low frequency%2Frare variants contributing to disease phenotypes. These will be based on obtaining the genome wide sequence of 4000 samples from the TwinsUK and ALSPAC cohorts (at 6x sequence coverage)%2C and the exome sequence (protein coding regions and related conserved sequence) of 6000 samples selected for extreme phenotypes. Our studies will focus primarily on cardiovascular-related quantitative traits%2C obesity and related metabolic traits%2C neurodevelopmental disorders and a limited number of extreme clinical phenotypes that will provide proof-of-concept for future familial trait sequencing. We will analyse directly quantitative traits in the cohorts and the selected traits in the extreme samples%2C and also use imputation down to 0.1%25 allele frequency to extend the analyses to further sample sets with genome wide genotype data. In each case we will investigate indels and larger structural variants as well as SNPs%2C and use statistical methods that combine rare variants in a locus or pathway as well as single-variant approaches. %0A%0AThe UK schizophrenia samples will be part of the neurodevelopmental disease group%2C and will undergo exome sequencing. ,1679,UK10K_NEURO_UKSCZ,Homo_sapiens (1000Genomes),,UK10K exome sequence%3A UK schizophrenia samples,8, +8,4405872,phiX_for_spiked_buffers,,ACAACGCAAT,,,ACAACGCAAT,,hps@sanger.ac.uk,,hps@sanger.ac.uk,hps@sanger.ac.uk,,1,0,,,PhiX (10Jan12),,,,,,standard,,,,,,,,,,,1255141,,phiX_for_spiked_buffers,,,,168,,1,0,0,None,198,Illumina Controls,,,,168, diff --git a/t/data/samplesheet/dual_index_extended.csv b/t/data/samplesheet/dual_index_extended.csv index 45188e6d..0384ab8f 100644 --- a/t/data/samplesheet/dual_index_extended.csv +++ b/t/data/samplesheet/dual_index_extended.csv @@ -1,8 +1,8 @@ -[Data],,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, -Lane,Sample_ID,Sample_Name,GenomeFolder,Index,Index2,bait_name,default_library_type,default_tag_sequence,default_tagtwo_sequence,email_addresses,email_addresses_of_followers,email_addresses_of_managers,email_addresses_of_owners,gbs_plex_name,is_control,is_pool,lane_id,lane_priority,library_name,organism,organism_taxon_id,project_cost_code,project_id,project_name,purpose,qc_state,request_id,required_insert_size_range,sample_accession_number,sample_cohort,sample_common_name,sample_consent_withdrawn,sample_description,sample_donor_id,sample_id,sample_name,sample_public_name,sample_reference_genome,sample_supplier_name,spiked_phix_tag_index,study_accession_number,study_alignments_in_bam,study_contains_nonconsented_human,study_contains_nonconsented_xahuman,study_description,study_id,study_name,study_reference_genome,study_separate_y_chromosome_data,study_title,tag_index, -1,6233,phiX_for_spiked_buffers,,CGATGTTT,AAAAAAAA,,Standard,CGATGTTT,AAAAAAAA,hr1@sanger.ac.uk jc17@sanger.ac.uk neh@sanger.ac.uk,jc17@sanger.ac.uk neh@sanger.ac.uk,neh@sanger.ac.uk,hr1@sanger.ac.uk,,0,0,,,,,,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:400 to:600,,,,,,,1255141,phiX_for_spiked_buffers,,,,,ERP000430,,0,0,Two H. contortus ivermectin resistance strains have been backcrossed 4 times against the susceptible genome strain. Parental strains and backcross strains will be sequenced in order to identify regions of the genome linked to ivermectin resistance-conferring loci.,1697,Haemonchus contortus Ivermectin Resistance,,,Haemonchus contortus Ivermectin Resistance,1, -1,6245,Strongyloides ratti,,TTAGGCAT,AAAAAAAA,,Standard,TTAGGCAT,AAAAAAAA,hr1@sanger.ac.uk jc17@sanger.ac.uk neh@sanger.ac.uk,jc17@sanger.ac.uk neh@sanger.ac.uk,neh@sanger.ac.uk,hr1@sanger.ac.uk,,0,0,,,,Strongyloides ratti,34506,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:400 to:600,,,Strongyloides ratti,,,,1289830,SriL3_66K_212889,Strongyloides ratti,Strongyloides_ratti (20100601),,,ERP000430,,0,0,Two H. contortus ivermectin resistance strains have been backcrossed 4 times against the susceptible genome strain. Parental strains and backcross strains will be sequenced in order to identify regions of the genome linked to ivermectin resistance-conferring loci.,1697,Haemonchus contortus Ivermectin Resistance,,,Haemonchus contortus Ivermectin Resistance,2, -1,6257,Salmonella pullorum,,TGACCACT,AAAAAAAA,,Standard,TGACCACT,AAAAAAAA,hr1@sanger.ac.uk jc17@sanger.ac.uk neh@sanger.ac.uk,jc17@sanger.ac.uk neh@sanger.ac.uk,neh@sanger.ac.uk,hr1@sanger.ac.uk,,0,0,,,,Salmonella pullorum,590,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:400 to:600,,,Salmonella pullorum,,genomic DNA,,1289832,sp200shear,Salmonella pullorum,Salmonella_pullorum (449_87),,,ERP000430,,0,0,Two H. contortus ivermectin resistance strains have been backcrossed 4 times against the susceptible genome strain. Parental strains and backcross strains will be sequenced in order to identify regions of the genome linked to ivermectin resistance-conferring loci.,1697,Haemonchus contortus Ivermectin Resistance,,,Haemonchus contortus Ivermectin Resistance,3, -2,8168,Bordetella Pertussis,,GCTAACTC,GGGGGGGG,,Standard,GCTAACTC,GGGGGGGG,nh4@sanger.ac.uk,,nh4@sanger.ac.uk,nh4@sanger.ac.uk,,0,0,,,,Bordetella Pertussis,520,S1553,1238,Mouse model to quantify genotype-epigenotype variations,standard,,,from:400 to:600,,,Bordetella Pertussis,,genomic DNA,,1289833,bp200shear,Bordetella Pertussis,Bordetella_pertussis (ST24),,,,1,0,0,R%26D,1980,Mate Pair R%26D,,,Mate Pair R%26D,374, -2,8180,Plasmodium Falciparum,,GATTCATC,GGGGGGGG,,Standard,GATTCATC,GGGGGGGG,nh4@sanger.ac.uk,,nh4@sanger.ac.uk,nh4@sanger.ac.uk,,0,0,,,,Plasmodium Falciparum,5820,S1553,1238,Mouse model to quantify genotype-epigenotype variations,standard,,,from:400 to:600,,,Plasmodium Falciparum,,genomic DNA,,1289834,3D7200shear,Plasmodium Falciparum,Plasmodium_falciparum (3D7),,,,1,0,0,R%26D,1980,Mate Pair R%26D,,,Mate Pair R%26D,375, -2,8192,Homo sapiens,,GTCTTGGC,GGGGGGGG,,Standard,GTCTTGGC,GGGGGGGG,nh4@sanger.ac.uk,,nh4@sanger.ac.uk,nh4@sanger.ac.uk,,0,0,,,,Homo sapiens,9606,S1553,1238,Mouse model to quantify genotype-epigenotype variations,standard,,,from:400 to:600,,,Human,,genomic DNA,,1289835,human200shear,Homo sapiens,Homo_sapiens (GRCh37_53),,,,1,0,0,R%26D,1980,Mate Pair R%26D,,,Mate Pair R%26D,376, +[Data],,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +Lane,Sample_ID,Sample_Name,GenomeFolder,Index,Index2,bait_name,default_library_type,default_tag_sequence,default_tagtwo_sequence,email_addresses,email_addresses_of_followers,email_addresses_of_managers,email_addresses_of_owners,gbs_plex_name,is_control,is_pool,lane_id,lane_priority,library_name,organism,organism_taxon_id,project_cost_code,project_id,project_name,purpose,qc_state,request_id,required_insert_size_range,sample_accession_number,sample_cohort,sample_common_name,sample_consent_withdrawn,sample_control_type,sample_description,sample_donor_id,sample_id,sample_is_control,sample_name,sample_public_name,sample_reference_genome,sample_supplier_name,spiked_phix_tag_index,study_accession_number,study_alignments_in_bam,study_contains_nonconsented_human,study_contains_nonconsented_xahuman,study_description,study_id,study_name,study_reference_genome,study_separate_y_chromosome_data,study_title,tag_index, +1,6233,phiX_for_spiked_buffers,,CGATGTTT,AAAAAAAA,,Standard,CGATGTTT,AAAAAAAA,hr1@sanger.ac.uk jc17@sanger.ac.uk neh@sanger.ac.uk,jc17@sanger.ac.uk neh@sanger.ac.uk,neh@sanger.ac.uk,hr1@sanger.ac.uk,,0,0,,,,,,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:400 to:600,,,,,,,,1255141,,phiX_for_spiked_buffers,,,,,ERP000430,,0,0,Two H. contortus ivermectin resistance strains have been backcrossed 4 times against the susceptible genome strain. Parental strains and backcross strains will be sequenced in order to identify regions of the genome linked to ivermectin resistance-conferring loci.,1697,Haemonchus contortus Ivermectin Resistance,,,Haemonchus contortus Ivermectin Resistance,1, +1,6245,Strongyloides ratti,,TTAGGCAT,AAAAAAAA,,Standard,TTAGGCAT,AAAAAAAA,hr1@sanger.ac.uk jc17@sanger.ac.uk neh@sanger.ac.uk,jc17@sanger.ac.uk neh@sanger.ac.uk,neh@sanger.ac.uk,hr1@sanger.ac.uk,,0,0,,,,Strongyloides ratti,34506,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:400 to:600,,,Strongyloides ratti,,,,,1289830,,SriL3_66K_212889,Strongyloides ratti,Strongyloides_ratti (20100601),,,ERP000430,,0,0,Two H. contortus ivermectin resistance strains have been backcrossed 4 times against the susceptible genome strain. Parental strains and backcross strains will be sequenced in order to identify regions of the genome linked to ivermectin resistance-conferring loci.,1697,Haemonchus contortus Ivermectin Resistance,,,Haemonchus contortus Ivermectin Resistance,2, +1,6257,Salmonella pullorum,,TGACCACT,AAAAAAAA,,Standard,TGACCACT,AAAAAAAA,hr1@sanger.ac.uk jc17@sanger.ac.uk neh@sanger.ac.uk,jc17@sanger.ac.uk neh@sanger.ac.uk,neh@sanger.ac.uk,hr1@sanger.ac.uk,,0,0,,,,Salmonella pullorum,590,S0917,1366,Single-cell analysis of the transcriptomic heterogeneity of ground state pluripotency,standard,,,from:400 to:600,,,Salmonella pullorum,,,genomic DNA,,1289832,,sp200shear,Salmonella pullorum,Salmonella_pullorum (449_87),,,ERP000430,,0,0,Two H. contortus ivermectin resistance strains have been backcrossed 4 times against the susceptible genome strain. Parental strains and backcross strains will be sequenced in order to identify regions of the genome linked to ivermectin resistance-conferring loci.,1697,Haemonchus contortus Ivermectin Resistance,,,Haemonchus contortus Ivermectin Resistance,3, +2,8168,Bordetella Pertussis,,GCTAACTC,GGGGGGGG,,Standard,GCTAACTC,GGGGGGGG,nh4@sanger.ac.uk,,nh4@sanger.ac.uk,nh4@sanger.ac.uk,,0,0,,,,Bordetella Pertussis,520,S1553,1238,Mouse model to quantify genotype-epigenotype variations,standard,,,from:400 to:600,,,Bordetella Pertussis,,,genomic DNA,,1289833,,bp200shear,Bordetella Pertussis,Bordetella_pertussis (ST24),,,,1,0,0,R%26D,1980,Mate Pair R%26D,,,Mate Pair R%26D,374, +2,8180,Plasmodium Falciparum,,GATTCATC,GGGGGGGG,,Standard,GATTCATC,GGGGGGGG,nh4@sanger.ac.uk,,nh4@sanger.ac.uk,nh4@sanger.ac.uk,,0,0,,,,Plasmodium Falciparum,5820,S1553,1238,Mouse model to quantify genotype-epigenotype variations,standard,,,from:400 to:600,,,Plasmodium Falciparum,,,genomic DNA,,1289834,,3D7200shear,Plasmodium Falciparum,Plasmodium_falciparum (3D7),,,,1,0,0,R%26D,1980,Mate Pair R%26D,,,Mate Pair R%26D,375, +2,8192,Homo sapiens,,GTCTTGGC,GGGGGGGG,,Standard,GTCTTGGC,GGGGGGGG,nh4@sanger.ac.uk,,nh4@sanger.ac.uk,nh4@sanger.ac.uk,,0,0,,,,Homo sapiens,9606,S1553,1238,Mouse model to quantify genotype-epigenotype variations,standard,,,from:400 to:600,,,Human,,,genomic DNA,,1289835,,human200shear,Homo sapiens,Homo_sapiens (GRCh37_53),,,,1,0,0,R%26D,1980,Mate Pair R%26D,,,Mate Pair R%26D,376, diff --git a/t/data/samplesheet/novaseq_multirun.csv b/t/data/samplesheet/novaseq_multirun.csv index bab08e37..4085771d 100644 --- a/t/data/samplesheet/novaseq_multirun.csv +++ b/t/data/samplesheet/novaseq_multirun.csv @@ -1,10 +1,10 @@ -[Data],,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, -Lane,Sample_ID,Sample_Name,GenomeFolder,bait_name,default_library_type,default_tag_sequence,default_tagtwo_sequence,email_addresses,email_addresses_of_followers,email_addresses_of_managers,email_addresses_of_owners,gbs_plex_name,id_run,is_control,is_pool,lane_id,lane_priority,library_name,organism,organism_taxon_id,project_cost_code,project_id,project_name,purpose,qc_state,request_id,required_insert_size_range,sample_accession_number,sample_cohort,sample_common_name,sample_consent_withdrawn,sample_description,sample_donor_id,sample_id,sample_name,sample_public_name,sample_reference_genome,sample_supplier_name,spiked_phix_tag_index,study_accession_number,study_alignments_in_bam,study_contains_nonconsented_human,study_contains_nonconsented_xahuman,study_description,study_id,study_name,study_reference_genome,study_separate_y_chromosome_data,study_title,tag_index, -1,22802061,7592352,,,HiSeqX PCR free,AGTTCAGG,CCAACAGA,aaaa@sanger.ac.uk,,aaaa@sanger.ac.uk,aaaa@sanger.ac.uk,,26480,0,0,22863688,0,22802061,,9606,T4600,,,standard,1,,from:450 to:450,,,Homo sapiens,0,,7592352,3811339,7592352,,,3547031,888,,1,0,0,UK Whole Genome sequencing study,5392,UK Study,Homo_sapiens (GRCh38_15_plus_hs38d1) %5Bminimap2%5D,0,UK Study,9, -2,22802061,7592352,,,HiSeqX PCR free,AGTTCAGG,CCAACAGA,aaaa@sanger.ac.uk,,aaaa@sanger.ac.uk,aaaa@sanger.ac.uk,,26480,0,0,22863689,0,22802061,,9606,T4600,,,standard,1,,from:450 to:450,,,Homo sapiens,0,,7592352,3811339,7592352,,,3547031,888,,1,0,0,UK Whole Genome sequencing study,5392,UK Study,Homo_sapiens (GRCh38_15_plus_hs38d1) %5Bminimap2%5D,0,UK Study,9, -3,22802061,7592352,,,HiSeqX PCR free,AGTTCAGG,CCAACAGA,aaaa@sanger.ac.uk,,aaaa@sanger.ac.uk,aaaa@sanger.ac.uk,,26480,0,0,22863690,0,22802061,,9606,T4600,,,standard,1,,from:450 to:450,,,Homo sapiens,0,,7592352,3811339,7592352,,,3547031,888,,1,0,0,UK Whole Genome sequencing study,5392,UK Study,Homo_sapiens (GRCh38_15_plus_hs38d1) %5Bminimap2%5D,0,UK Study,9, -4,22802061,7592352,,,HiSeqX PCR free,AGTTCAGG,CCAACAGA,aaaa@sanger.ac.uk,,aaaa@sanger.ac.uk,aaaa@sanger.ac.uk,,26480,0,0,22863691,0,22802061,,9606,T4600,,,standard,1,,from:450 to:450,,,Homo sapiens,0,,7592352,3811339,7592352,,,3547031,888,,1,0,0,UK Whole Genome sequencing study,5392,UK Study,Homo_sapiens (GRCh38_15_plus_hs38d1) %5Bminimap2%5D,0,UK Study,9, -1,23356155,7617423,,,HiSeqX PCR free,GGCTTAAG,TCGTGACC,aaaa@sanger.ac.uk,,aaaa@sanger.ac.uk,aaaa@sanger.ac.uk,,28780,0,0,25222813,0,23356155,,9606,T4600,,,standard,1,,from:450 to:450,,,Homo sapiens,0,,7617423,3836420,7617423,,,3426258,888,,1,0,0,UK Whole Genome sequencing study,5392,UK Study,Homo_sapiens (GRCh38_15_plus_hs38d1) %5Bminimap2%5D,0,UK Study,4, -2,22802061,7592352,,,HiSeqX PCR free,AGTTCAGG,CCAACAGA,aaaa@sanger.ac.uk,,aaaa@sanger.ac.uk,aaaa@sanger.ac.uk,,28780,0,0,25222814,0,22802061,,9606,T4600,,,standard,1,,from:450 to:450,,,Homo sapiens,0,,7592352,3811339,7592352,,,3547031,888,,1,0,0,UK Whole Genome sequencing study,5392,UK Study,Homo_sapiens (GRCh38_15_plus_hs38d1) %5Bminimap2%5D,0,UK Study,4, -3,23455444,7616265,,,HiSeqX PCR free,AGTTCAGG,CCAACAGA,aaaa@sanger.ac.uk,,aaaa@sanger.ac.uk,aaaa@sanger.ac.uk,,28780,0,0,25222815,0,23455444,,9606,T4600,,,standard,1,,from:450 to:450,,,Homo sapiens,0,,7616265,3835262,7616265,,,2612688,888,,1,0,0,UK Whole Genome sequencing study,5392,UK Study,Homo_sapiens (GRCh38_15_plus_hs38d1) %5Bminimap2%5D,0,UK Study,4, -4,23454971,7615018,,,HiSeqX PCR free,GACCTGAA,TTGGTGAG,aaaa@sanger.ac.uk,,aaaa@sanger.ac.uk,aaaa@sanger.ac.uk,,28780,0,0,25222816,0,23454971,,9606,T4600,,,standard,1,,from:450 to:450,,,Homo sapiens,0,,7615018,3834015,7615018,,,2361066,888,,1,0,0,UK Whole Genome sequencing study,5392,UK Study,Homo_sapiens (GRCh38_15_plus_hs38d1) %5Bminimap2%5D,0,UK Study,4, +[Data],,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +Lane,Sample_ID,Sample_Name,GenomeFolder,bait_name,default_library_type,default_tag_sequence,default_tagtwo_sequence,email_addresses,email_addresses_of_followers,email_addresses_of_managers,email_addresses_of_owners,gbs_plex_name,id_run,is_control,is_pool,lane_id,lane_priority,library_name,organism,organism_taxon_id,project_cost_code,project_id,project_name,purpose,qc_state,request_id,required_insert_size_range,sample_accession_number,sample_cohort,sample_common_name,sample_consent_withdrawn,sample_control_type,sample_description,sample_donor_id,sample_id,sample_is_control,sample_name,sample_public_name,sample_reference_genome,sample_supplier_name,spiked_phix_tag_index,study_accession_number,study_alignments_in_bam,study_contains_nonconsented_human,study_contains_nonconsented_xahuman,study_description,study_id,study_name,study_reference_genome,study_separate_y_chromosome_data,study_title,tag_index, +1,22802061,7592352,,,HiSeqX PCR free,AGTTCAGG,CCAACAGA,aaaa@sanger.ac.uk,,aaaa@sanger.ac.uk,aaaa@sanger.ac.uk,,26480,0,0,22863688,0,22802061,,9606,T4600,,,standard,1,,from:450 to:450,,,Homo sapiens,0,,,7592352,3811339,,7592352,,,3547031,888,,1,0,0,UK Whole Genome sequencing study,5392,UK Study,Homo_sapiens (GRCh38_15_plus_hs38d1) %5Bminimap2%5D,0,UK Study,9, +2,22802061,7592352,,,HiSeqX PCR free,AGTTCAGG,CCAACAGA,aaaa@sanger.ac.uk,,aaaa@sanger.ac.uk,aaaa@sanger.ac.uk,,26480,0,0,22863689,0,22802061,,9606,T4600,,,standard,1,,from:450 to:450,,,Homo sapiens,0,,,7592352,3811339,,7592352,,,3547031,888,,1,0,0,UK Whole Genome sequencing study,5392,UK Study,Homo_sapiens (GRCh38_15_plus_hs38d1) %5Bminimap2%5D,0,UK Study,9, +3,22802061,7592352,,,HiSeqX PCR free,AGTTCAGG,CCAACAGA,aaaa@sanger.ac.uk,,aaaa@sanger.ac.uk,aaaa@sanger.ac.uk,,26480,0,0,22863690,0,22802061,,9606,T4600,,,standard,1,,from:450 to:450,,,Homo sapiens,0,,,7592352,3811339,,7592352,,,3547031,888,,1,0,0,UK Whole Genome sequencing study,5392,UK Study,Homo_sapiens (GRCh38_15_plus_hs38d1) %5Bminimap2%5D,0,UK Study,9, +4,22802061,7592352,,,HiSeqX PCR free,AGTTCAGG,CCAACAGA,aaaa@sanger.ac.uk,,aaaa@sanger.ac.uk,aaaa@sanger.ac.uk,,26480,0,0,22863691,0,22802061,,9606,T4600,,,standard,1,,from:450 to:450,,,Homo sapiens,0,,,7592352,3811339,,7592352,,,3547031,888,,1,0,0,UK Whole Genome sequencing study,5392,UK Study,Homo_sapiens (GRCh38_15_plus_hs38d1) %5Bminimap2%5D,0,UK Study,9, +1,23356155,7617423,,,HiSeqX PCR free,GGCTTAAG,TCGTGACC,aaaa@sanger.ac.uk,,aaaa@sanger.ac.uk,aaaa@sanger.ac.uk,,28780,0,0,25222813,0,23356155,,9606,T4600,,,standard,1,,from:450 to:450,,,Homo sapiens,0,,,7617423,3836420,,7617423,,,3426258,888,,1,0,0,UK Whole Genome sequencing study,5392,UK Study,Homo_sapiens (GRCh38_15_plus_hs38d1) %5Bminimap2%5D,0,UK Study,4, +2,22802061,7592352,,,HiSeqX PCR free,AGTTCAGG,CCAACAGA,aaaa@sanger.ac.uk,,aaaa@sanger.ac.uk,aaaa@sanger.ac.uk,,28780,0,0,25222814,0,22802061,,9606,T4600,,,standard,1,,from:450 to:450,,,Homo sapiens,0,,,7592352,3811339,,7592352,,,3547031,888,,1,0,0,UK Whole Genome sequencing study,5392,UK Study,Homo_sapiens (GRCh38_15_plus_hs38d1) %5Bminimap2%5D,0,UK Study,4, +3,23455444,7616265,,,HiSeqX PCR free,AGTTCAGG,CCAACAGA,aaaa@sanger.ac.uk,,aaaa@sanger.ac.uk,aaaa@sanger.ac.uk,,28780,0,0,25222815,0,23455444,,9606,T4600,,,standard,1,,from:450 to:450,,,Homo sapiens,0,,,7616265,3835262,,7616265,,,2612688,888,,1,0,0,UK Whole Genome sequencing study,5392,UK Study,Homo_sapiens (GRCh38_15_plus_hs38d1) %5Bminimap2%5D,0,UK Study,4, +4,23454971,7615018,,,HiSeqX PCR free,GACCTGAA,TTGGTGAG,aaaa@sanger.ac.uk,,aaaa@sanger.ac.uk,aaaa@sanger.ac.uk,,28780,0,0,25222816,0,23454971,,9606,T4600,,,standard,1,,from:450 to:450,,,Homo sapiens,0,,,7615018,3834015,,7615018,,,2361066,888,,1,0,0,UK Whole Genome sequencing study,5392,UK Study,Homo_sapiens (GRCh38_15_plus_hs38d1) %5Bminimap2%5D,0,UK Study,4, From 0f9be2ee37425e1f0893522a33eaf2da5754b980 Mon Sep 17 00:00:00 2001 From: mgcam Date: Fri, 12 Jun 2020 13:17:17 +0100 Subject: [PATCH 4/4] update Changes file for release 91.4.0 --- Changes | 1 + 1 file changed, 1 insertion(+) diff --git a/Changes b/Changes index 640a6ade..b0537dc7 100644 --- a/Changes +++ b/Changes @@ -1,5 +1,6 @@ LIST OF CHANGES +release 91.4.0 - disable downloading *.fa (consensus) files from tracking server views of staging folders - add executable for npg samplesheet4MiSeq wrapper