The FAST Analysis of Sequences Toolbox (FAST) is a set of Unix tools (for example fasgrep, fascut, fashead and fastr) for sequence bioinformatics modeled after the Unix textutils (such as grep, cut, head, tr, etc). FAST workflows are designed for "inline" (serial) processing of flatfile biological sequence record databases per-sequence, rather than per-line, through Unix command pipelines. The default data exchange format is multifasta (specifically, a restriction of BioPerl FastA format). FAST tools expose the power of Perl and BioPerl for sequence analysis to non-programmers in an easy-to-learn command-line paradigm.
You do not need to know Perl or BioPerl to use FAST.
FAST 1.6 contains the following utilities. Each has its own man page. FAST utilities may be classifed as for annotation, selection, transformation, and analysis.
| Program | Description |
|---|---|
| faslen | annotate sequence lengths |
| fascodon | tally/annotate codon usage |
| fascomp | tally/annotate monomer frequencies |
| fasxl | translate gapped and ungapped sequences and alignments |
| fasrc | reverse complement nucleotide sequences and alignments |
| Program | Description |
|---|---|
| fasgrep | select sequence records by perl regular expressions |
| fasfilter | select sequence records by numerical values |
| fastax | select sequence records by NCBI Taxonomy IDs or names |
| fascut | select/reorder sequence record data by sequential ranges |
| fasuniq | remove duplicate sequence records from sorted data |
| fashead | select leading sequence records |
| fastail | select trailing sequence records |
| alncut | select sites based on variation and gap-content content |
| gbfcut | select sequences by regex match on features in a GenBank features |
| gbfalncut | select sites by regex match on features in a GenBank features |
| Program | Description |
|---|---|
| fasconvert | convert sequences to or from from fasta format |
| fassort | sort sequence records |
| fastaxsort | sort sequence records by NCBI Taxonomy IDs or names |
| faspaste | concatenate sequence record data |
| fastr | transform sequence records by characters and alphabets |
| fassub | transform sequence records by regex-based substitutions |
| Program | Description |
|---|---|
| faswc | tally sequences and characters |
| alnpi | tally molecular population genetic statistics |
FAST has some Perl dependencies. Once posted to CPAN, the easiest way to install FAST with its dependencies will be by executing this command:
perl -MCPAN -e 'install FAST'You can also follow INSTALLATION instructions below, but you may need to first run these commands to install FAST dependencies:
perl -MPCAN -e 'install Sort::Key'
perl -MCPAN -e 'install Sort::MergeSort'
perl -MCPAN -e 'install Bit::Vector'
perl -MCPAN -e 'install Test::More'
perl -MCPAN -e 'install Test::Script::Run'To install this module, run the following:
perl Makefile.PL
make
make test
(sudo) make installIf you haven't installed perl-based software on your system before, or you are having problems, please see more detailed notes in INSTALL.
perldoc fasgrep
man fasgrep
perldoc FASTSome usage examples in the man pages refer to data that ships with FAST, in the installation package under t/data. Run these examples with these data to get started right away using FAST.
Installation generates a man page for each utility. From the installation directory, additional resources are in ./doc, particularly the FAST_Cookbook
You can also look for information at:
RT, CPAN's request tracker (report bugs here):
http://rt.cpan.org/NoAuth/Bugs.html?Dist=FAST
AnnoCPAN, Annotated CPAN documentation:
CPAN Ratings:
http://cpanratings.perl.org/d/FAST
Search CPAN:
http://search.cpan.org/dist/FAST/
If you use FAST please, cite the FAST project (Lawrence et al. 2015) and Bioperl (Stajich et al. 2005).
If you publish work that cites data provided in this FAST installer, please cite the authors of this data accordingly. For example, if you use the tRNAdb-CE data provided in this installer, please cite: Abe T, Inokuchi H, Yamada Y, Muto A, Iwasaki Y and Ikemura T (2014) tRNADB-CE: tRNA gene database well-timed in the era of big sequence data. Front. Genet. 5:114. doi: 10.3389/fgene.2014.00114
1.0 -- First public release
Copyright (C) 2015 David H. Ardell
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