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Add col for qPCR dilution vol #381

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merged 2 commits into from
Nov 8, 2024
Merged

Add col for qPCR dilution vol #381

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b117e89
Add col for qPCR dilution vol
chuan-wang Nov 8, 2024
dc30206
Add col for qPCR dilution vol
chuan-wang Nov 8, 2024
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4 changes: 4 additions & 0 deletions VERSIONLOG.md
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Original file line number Diff line number Diff line change
@@ -1,5 +1,9 @@
# Scilifelab_epps Version Log

## 20241108.1

Add col for qPCR dilution vol

## 20241104.2

For AVITI manifest generation: make PhiX manifest variant, fix udf typo, remove unused func, clarify var names, add cases to reverse-compliment Index2.
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10 changes: 8 additions & 2 deletions scripts/bravo_csv.py
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Expand Up @@ -18,6 +18,7 @@

MAX_WARNING_VOLUME = 150.0
MIN_WARNING_VOLUME = 2.0
QPCR_DILUTION_VOLUME = 40

# Three values are minimum required conc for setup workset, maximum conc for dilution and minimum volume for dilution
Dilution_preset = {"Smarter pico": [1.25, 375.0, 10.0]}
Expand Down Expand Up @@ -337,8 +338,13 @@ def setup_qpcr(currentStep, lims):
with open("bravo.csv", "w") as csvContext:
for s in data:
csvContext.write(
"{},{},{},{},{}\n".format(
s["src_fc_id"], s["src_well"], s["vol"], s["dst_fc"], s["dst_well"]
"{},{},{},{},{},{}\n".format(
s["src_fc_id"],
s["src_well"],
s["vol"],
s["dst_fc"],
s["dst_well"],
QPCR_DILUTION_VOLUME,
)
)
if log:
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