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Update README.md
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johausmann authored Oct 24, 2023
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Expand Up @@ -236,6 +236,12 @@ Other low quality mutations are removed from the output.
The VAF thresholds can be changed with the parameters `--low_frequency_variant_threshold`,
`--subclonal_variant_threshold` and `--low_quality_clonal_variant_threshold`.

### Lineage only mode

At some point, it might be desirable to run only the lineage determination to obtain only the pangolin calls
of a sample. To solve this problem, we have implemented the lineage-only mode, which can be executed by specifying the option
"--lineage_mode" option. Please note that this mode can only be used with FASTA or VCF as input.

## How to run

### Requirements
Expand Down Expand Up @@ -387,6 +393,17 @@ path to BAM and path to BAI.
| ... | ... | ... |


For lineage-only:
```
nextflow run tron-bioinformatics/covigator-ngs-pipeline \
[-r v0.10.0] \
[-profile conda] \
--fasta <FASTA_FILE> \
--name example_run \
--output <OUTPUT_FOLDER> \
--lineage_mode
```


### Getting help

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